Evidence of Circulation of Several HAV Genetic Variants and Emergence of Potential Antigenic Variants in an Endemo-Epidemic Country before Vaccine Introduction

Similar to several other countries in the world, the epidemiology of hepatitis A virus changed from high to intermediate endemicity level in Tunisia, which led to the occurrence of outbreaks. This study aimed to determine the genetic and antigenic variability of HAV strains circulating in Tunisia du...

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Autores principales: Ayouni, Kaouther, Chouikha, Anissa, Khamessi, Oussema, Touzi, Henda, Hammemi, Walid, Triki, Henda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8227776/
https://www.ncbi.nlm.nih.gov/pubmed/34204862
http://dx.doi.org/10.3390/v13061056
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author Ayouni, Kaouther
Chouikha, Anissa
Khamessi, Oussema
Touzi, Henda
Hammemi, Walid
Triki, Henda
author_facet Ayouni, Kaouther
Chouikha, Anissa
Khamessi, Oussema
Touzi, Henda
Hammemi, Walid
Triki, Henda
author_sort Ayouni, Kaouther
collection PubMed
description Similar to several other countries in the world, the epidemiology of hepatitis A virus changed from high to intermediate endemicity level in Tunisia, which led to the occurrence of outbreaks. This study aimed to determine the genetic and antigenic variability of HAV strains circulating in Tunisia during the last few years. Genotyping using complete VP1 gene and VP1-2A junction confirmed the predominance of genotype IA, with co-circulation of several genetic and antigenic variants. Phylogenetic analysis including Tunisian and strains from other regions of the world showed the presence of at least two IA-variants within IA subgenotype. Amino-acid analysis showed several mutations in or close to epitope regions in the VP1-region. This study provides a baseline on the genetic and antigenic variability of HAV circulating strains before the introduction of vaccination into the national immunization schedule.
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spelling pubmed-82277762021-06-26 Evidence of Circulation of Several HAV Genetic Variants and Emergence of Potential Antigenic Variants in an Endemo-Epidemic Country before Vaccine Introduction Ayouni, Kaouther Chouikha, Anissa Khamessi, Oussema Touzi, Henda Hammemi, Walid Triki, Henda Viruses Article Similar to several other countries in the world, the epidemiology of hepatitis A virus changed from high to intermediate endemicity level in Tunisia, which led to the occurrence of outbreaks. This study aimed to determine the genetic and antigenic variability of HAV strains circulating in Tunisia during the last few years. Genotyping using complete VP1 gene and VP1-2A junction confirmed the predominance of genotype IA, with co-circulation of several genetic and antigenic variants. Phylogenetic analysis including Tunisian and strains from other regions of the world showed the presence of at least two IA-variants within IA subgenotype. Amino-acid analysis showed several mutations in or close to epitope regions in the VP1-region. This study provides a baseline on the genetic and antigenic variability of HAV circulating strains before the introduction of vaccination into the national immunization schedule. MDPI 2021-06-03 /pmc/articles/PMC8227776/ /pubmed/34204862 http://dx.doi.org/10.3390/v13061056 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ayouni, Kaouther
Chouikha, Anissa
Khamessi, Oussema
Touzi, Henda
Hammemi, Walid
Triki, Henda
Evidence of Circulation of Several HAV Genetic Variants and Emergence of Potential Antigenic Variants in an Endemo-Epidemic Country before Vaccine Introduction
title Evidence of Circulation of Several HAV Genetic Variants and Emergence of Potential Antigenic Variants in an Endemo-Epidemic Country before Vaccine Introduction
title_full Evidence of Circulation of Several HAV Genetic Variants and Emergence of Potential Antigenic Variants in an Endemo-Epidemic Country before Vaccine Introduction
title_fullStr Evidence of Circulation of Several HAV Genetic Variants and Emergence of Potential Antigenic Variants in an Endemo-Epidemic Country before Vaccine Introduction
title_full_unstemmed Evidence of Circulation of Several HAV Genetic Variants and Emergence of Potential Antigenic Variants in an Endemo-Epidemic Country before Vaccine Introduction
title_short Evidence of Circulation of Several HAV Genetic Variants and Emergence of Potential Antigenic Variants in an Endemo-Epidemic Country before Vaccine Introduction
title_sort evidence of circulation of several hav genetic variants and emergence of potential antigenic variants in an endemo-epidemic country before vaccine introduction
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8227776/
https://www.ncbi.nlm.nih.gov/pubmed/34204862
http://dx.doi.org/10.3390/v13061056
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