Cargando…
Lactiplantibacillus plantarum MG4296 and Lacticaseibacillus paracasei MG5012 Ameliorates Insulin Resistance in Palmitic Acid-Induced HepG2 Cells and High Fat Diet-Induced Mice
The purpose of this study was to evaluate the capacity of Lactiplantibacillus plantarum MG4296 (MG4296) and Lacticaseibacillus paracasei MG5012 (MG5012) on insulin resistance (IR) and diabetes-related metabolic changes in palmitic acid (PA)-induced HepG2 cells and high-fat diet-induced mice. In vitr...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8228052/ https://www.ncbi.nlm.nih.gov/pubmed/34070604 http://dx.doi.org/10.3390/microorganisms9061139 |
_version_ | 1783712652045844480 |
---|---|
author | Won, Gayeong Choi, Soo-Im Kang, Chang-Ho Kim, Gun-Hee |
author_facet | Won, Gayeong Choi, Soo-Im Kang, Chang-Ho Kim, Gun-Hee |
author_sort | Won, Gayeong |
collection | PubMed |
description | The purpose of this study was to evaluate the capacity of Lactiplantibacillus plantarum MG4296 (MG4296) and Lacticaseibacillus paracasei MG5012 (MG5012) on insulin resistance (IR) and diabetes-related metabolic changes in palmitic acid (PA)-induced HepG2 cells and high-fat diet-induced mice. In vitro, cell-free extracts of MG4296 and MG5012 alleviated IR by increasing glucose uptake and glycogen content in PA-induced insulin-resistant HepG2 cells. In vivo, MG4296 and MG5012 supplementation markedly decreased body weight and glucose tolerance. Administration of both strains also improved serum glucose, glycated hemoglobin, insulin, triglyceride, LDL/HDL ratio, and homeostatic model assessment of IR (HOMA-IR). Histopathological analysis of liver tissue demonstrated a significant reduction in lipid accumulation and glycogen content. Moreover, MG4296 and MG5012 treatment enhanced phosphoinositide-3 kinase (PI3K)/protein kinase B (Akt) expression in the liver. Overall, MG4296 and MG5012 could prevent HFD-induced glucose tolerance and hyperglycemia by improving IR. Therefore, L. plantarum MG4296 and L. paracasei MG5012 could be useful as new probiotics candidates to improve T2DM. |
format | Online Article Text |
id | pubmed-8228052 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-82280522021-06-26 Lactiplantibacillus plantarum MG4296 and Lacticaseibacillus paracasei MG5012 Ameliorates Insulin Resistance in Palmitic Acid-Induced HepG2 Cells and High Fat Diet-Induced Mice Won, Gayeong Choi, Soo-Im Kang, Chang-Ho Kim, Gun-Hee Microorganisms Article The purpose of this study was to evaluate the capacity of Lactiplantibacillus plantarum MG4296 (MG4296) and Lacticaseibacillus paracasei MG5012 (MG5012) on insulin resistance (IR) and diabetes-related metabolic changes in palmitic acid (PA)-induced HepG2 cells and high-fat diet-induced mice. In vitro, cell-free extracts of MG4296 and MG5012 alleviated IR by increasing glucose uptake and glycogen content in PA-induced insulin-resistant HepG2 cells. In vivo, MG4296 and MG5012 supplementation markedly decreased body weight and glucose tolerance. Administration of both strains also improved serum glucose, glycated hemoglobin, insulin, triglyceride, LDL/HDL ratio, and homeostatic model assessment of IR (HOMA-IR). Histopathological analysis of liver tissue demonstrated a significant reduction in lipid accumulation and glycogen content. Moreover, MG4296 and MG5012 treatment enhanced phosphoinositide-3 kinase (PI3K)/protein kinase B (Akt) expression in the liver. Overall, MG4296 and MG5012 could prevent HFD-induced glucose tolerance and hyperglycemia by improving IR. Therefore, L. plantarum MG4296 and L. paracasei MG5012 could be useful as new probiotics candidates to improve T2DM. MDPI 2021-05-25 /pmc/articles/PMC8228052/ /pubmed/34070604 http://dx.doi.org/10.3390/microorganisms9061139 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Won, Gayeong Choi, Soo-Im Kang, Chang-Ho Kim, Gun-Hee Lactiplantibacillus plantarum MG4296 and Lacticaseibacillus paracasei MG5012 Ameliorates Insulin Resistance in Palmitic Acid-Induced HepG2 Cells and High Fat Diet-Induced Mice |
title | Lactiplantibacillus plantarum MG4296 and Lacticaseibacillus paracasei MG5012 Ameliorates Insulin Resistance in Palmitic Acid-Induced HepG2 Cells and High Fat Diet-Induced Mice |
title_full | Lactiplantibacillus plantarum MG4296 and Lacticaseibacillus paracasei MG5012 Ameliorates Insulin Resistance in Palmitic Acid-Induced HepG2 Cells and High Fat Diet-Induced Mice |
title_fullStr | Lactiplantibacillus plantarum MG4296 and Lacticaseibacillus paracasei MG5012 Ameliorates Insulin Resistance in Palmitic Acid-Induced HepG2 Cells and High Fat Diet-Induced Mice |
title_full_unstemmed | Lactiplantibacillus plantarum MG4296 and Lacticaseibacillus paracasei MG5012 Ameliorates Insulin Resistance in Palmitic Acid-Induced HepG2 Cells and High Fat Diet-Induced Mice |
title_short | Lactiplantibacillus plantarum MG4296 and Lacticaseibacillus paracasei MG5012 Ameliorates Insulin Resistance in Palmitic Acid-Induced HepG2 Cells and High Fat Diet-Induced Mice |
title_sort | lactiplantibacillus plantarum mg4296 and lacticaseibacillus paracasei mg5012 ameliorates insulin resistance in palmitic acid-induced hepg2 cells and high fat diet-induced mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8228052/ https://www.ncbi.nlm.nih.gov/pubmed/34070604 http://dx.doi.org/10.3390/microorganisms9061139 |
work_keys_str_mv | AT wongayeong lactiplantibacillusplantarummg4296andlacticaseibacillusparacaseimg5012amelioratesinsulinresistanceinpalmiticacidinducedhepg2cellsandhighfatdietinducedmice AT choisooim lactiplantibacillusplantarummg4296andlacticaseibacillusparacaseimg5012amelioratesinsulinresistanceinpalmiticacidinducedhepg2cellsandhighfatdietinducedmice AT kangchangho lactiplantibacillusplantarummg4296andlacticaseibacillusparacaseimg5012amelioratesinsulinresistanceinpalmiticacidinducedhepg2cellsandhighfatdietinducedmice AT kimgunhee lactiplantibacillusplantarummg4296andlacticaseibacillusparacaseimg5012amelioratesinsulinresistanceinpalmiticacidinducedhepg2cellsandhighfatdietinducedmice |