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Genome-Wide Identification and Expression Analysis of the NRAMP Family Genes in Tea Plant (Camellia sinensis)

The natural resistant-associated macrophage protein (NRAMP) is a kind of integral membrane transporter which could function on a wide range of divalent metal ions in plants. Little is known about the NRAMP family in Camellia sinensis. In this study, 11 NRAMP genes were identified from the tea plant...

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Autores principales: Li, Jinqiu, Duan, Yu, Han, Zhaolan, Shang, Xiaowen, Zhang, Kexin, Zou, Zhongwei, Ma, Yuanchun, Li, Fang, Fang, Wanping, Zhu, Xujun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8228228/
https://www.ncbi.nlm.nih.gov/pubmed/34070434
http://dx.doi.org/10.3390/plants10061055
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author Li, Jinqiu
Duan, Yu
Han, Zhaolan
Shang, Xiaowen
Zhang, Kexin
Zou, Zhongwei
Ma, Yuanchun
Li, Fang
Fang, Wanping
Zhu, Xujun
author_facet Li, Jinqiu
Duan, Yu
Han, Zhaolan
Shang, Xiaowen
Zhang, Kexin
Zou, Zhongwei
Ma, Yuanchun
Li, Fang
Fang, Wanping
Zhu, Xujun
author_sort Li, Jinqiu
collection PubMed
description The natural resistant-associated macrophage protein (NRAMP) is a kind of integral membrane transporter which could function on a wide range of divalent metal ions in plants. Little is known about the NRAMP family in Camellia sinensis. In this study, 11 NRAMP genes were identified from the tea plant genome. Phylogenetic analysis showed that the 11 CsNRAMP proteins were split into two groups. The proteins of group 1 contained the conserved motif 6 (GQSSTxTG), while most proteins in group 2 (excepting CsNRAMP7 and CsNRAMP10) contained the conserved residues of motif 6 and motif 2 (GQFIMxGFLxLxxKKW). The number of amino acids in coding regions of 11 CsNRAMP genes ranged from 279–1373, and they contained 3–12 transmembrane domains. Quantitative RT-PCR analysis showed that G1 genes, CsNRAMP3, CsNRAMP4, and CsNRAMP5, were extraordinarily expressed in roots, while G2 genes showed higher expression levels in the stems and leaves. The expression levels of CsNRAMPs in roots and leaves were detected to assess their responses to Pb treatment. The results indicated that CsNRAMPs were differentially regulated, and they might play a role in Pb transportation of tea plant. Subcellular localization assay demonstrated that CsNRAMP2 and CsNRAMP5 fused proteins were localized in the plasma membrane. Overall, this systematic analysis of the CsNRAMP family could provide primary information for further studies on the functional roles of CsNRAMPs in divalent metal transportation in tea plants.
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spelling pubmed-82282282021-06-26 Genome-Wide Identification and Expression Analysis of the NRAMP Family Genes in Tea Plant (Camellia sinensis) Li, Jinqiu Duan, Yu Han, Zhaolan Shang, Xiaowen Zhang, Kexin Zou, Zhongwei Ma, Yuanchun Li, Fang Fang, Wanping Zhu, Xujun Plants (Basel) Article The natural resistant-associated macrophage protein (NRAMP) is a kind of integral membrane transporter which could function on a wide range of divalent metal ions in plants. Little is known about the NRAMP family in Camellia sinensis. In this study, 11 NRAMP genes were identified from the tea plant genome. Phylogenetic analysis showed that the 11 CsNRAMP proteins were split into two groups. The proteins of group 1 contained the conserved motif 6 (GQSSTxTG), while most proteins in group 2 (excepting CsNRAMP7 and CsNRAMP10) contained the conserved residues of motif 6 and motif 2 (GQFIMxGFLxLxxKKW). The number of amino acids in coding regions of 11 CsNRAMP genes ranged from 279–1373, and they contained 3–12 transmembrane domains. Quantitative RT-PCR analysis showed that G1 genes, CsNRAMP3, CsNRAMP4, and CsNRAMP5, were extraordinarily expressed in roots, while G2 genes showed higher expression levels in the stems and leaves. The expression levels of CsNRAMPs in roots and leaves were detected to assess their responses to Pb treatment. The results indicated that CsNRAMPs were differentially regulated, and they might play a role in Pb transportation of tea plant. Subcellular localization assay demonstrated that CsNRAMP2 and CsNRAMP5 fused proteins were localized in the plasma membrane. Overall, this systematic analysis of the CsNRAMP family could provide primary information for further studies on the functional roles of CsNRAMPs in divalent metal transportation in tea plants. MDPI 2021-05-25 /pmc/articles/PMC8228228/ /pubmed/34070434 http://dx.doi.org/10.3390/plants10061055 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Jinqiu
Duan, Yu
Han, Zhaolan
Shang, Xiaowen
Zhang, Kexin
Zou, Zhongwei
Ma, Yuanchun
Li, Fang
Fang, Wanping
Zhu, Xujun
Genome-Wide Identification and Expression Analysis of the NRAMP Family Genes in Tea Plant (Camellia sinensis)
title Genome-Wide Identification and Expression Analysis of the NRAMP Family Genes in Tea Plant (Camellia sinensis)
title_full Genome-Wide Identification and Expression Analysis of the NRAMP Family Genes in Tea Plant (Camellia sinensis)
title_fullStr Genome-Wide Identification and Expression Analysis of the NRAMP Family Genes in Tea Plant (Camellia sinensis)
title_full_unstemmed Genome-Wide Identification and Expression Analysis of the NRAMP Family Genes in Tea Plant (Camellia sinensis)
title_short Genome-Wide Identification and Expression Analysis of the NRAMP Family Genes in Tea Plant (Camellia sinensis)
title_sort genome-wide identification and expression analysis of the nramp family genes in tea plant (camellia sinensis)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8228228/
https://www.ncbi.nlm.nih.gov/pubmed/34070434
http://dx.doi.org/10.3390/plants10061055
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