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Identifying a Novel Bile Salt Hydrolase from the Keystone Gut Bacterium Christensenella minuta

Christensenella minuta are human gut dwelling bacteria that have been proposed as key members of the gut microbiome, regulating energy balance and adiposity of their host. We formerly identified that a novel strain of C. minuta (strain DSM33407) boosted microbiota diversity and stimulated deconjugat...

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Autores principales: Déjean, Guillaume, Tudela, Héloïse, Bruno, Lisa, Kissi, Déborah, Rawadi, Georges, Claus, Sandrine P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8228234/
https://www.ncbi.nlm.nih.gov/pubmed/34207623
http://dx.doi.org/10.3390/microorganisms9061252
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author Déjean, Guillaume
Tudela, Héloïse
Bruno, Lisa
Kissi, Déborah
Rawadi, Georges
Claus, Sandrine P.
author_facet Déjean, Guillaume
Tudela, Héloïse
Bruno, Lisa
Kissi, Déborah
Rawadi, Georges
Claus, Sandrine P.
author_sort Déjean, Guillaume
collection PubMed
description Christensenella minuta are human gut dwelling bacteria that have been proposed as key members of the gut microbiome, regulating energy balance and adiposity of their host. We formerly identified that a novel strain of C. minuta (strain DSM33407) boosted microbiota diversity and stimulated deconjugation of the primary bile acid taurocholic acid in human samples. However, there is no description of a bile salt hydrolase (BSH) protein carried in the genome of C. minuta. Here, we identified and cloned a protein from C. minuta’s genome that carries a potent BSH activity, which preferentially deconjugates glycine-conjugated bile acids. We then retrieved 14,319 putative BSH sequences from the NCBI database and filtered them using the UHGP database to collect a total of 6701 sequences that were used to build the most comprehensive phylogenetic tree of BSH-related enzymes identified in the human microbiome so far. This phylogenetic tree revealed that C. minuta’s BSH amino acid sequence clusters away from others with a threshold of 70% identity. This is therefore the first description of C. minuta’s BSH protein, which may be involved in its unique role within the human gut microbial ecosystem.
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spelling pubmed-82282342021-06-26 Identifying a Novel Bile Salt Hydrolase from the Keystone Gut Bacterium Christensenella minuta Déjean, Guillaume Tudela, Héloïse Bruno, Lisa Kissi, Déborah Rawadi, Georges Claus, Sandrine P. Microorganisms Article Christensenella minuta are human gut dwelling bacteria that have been proposed as key members of the gut microbiome, regulating energy balance and adiposity of their host. We formerly identified that a novel strain of C. minuta (strain DSM33407) boosted microbiota diversity and stimulated deconjugation of the primary bile acid taurocholic acid in human samples. However, there is no description of a bile salt hydrolase (BSH) protein carried in the genome of C. minuta. Here, we identified and cloned a protein from C. minuta’s genome that carries a potent BSH activity, which preferentially deconjugates glycine-conjugated bile acids. We then retrieved 14,319 putative BSH sequences from the NCBI database and filtered them using the UHGP database to collect a total of 6701 sequences that were used to build the most comprehensive phylogenetic tree of BSH-related enzymes identified in the human microbiome so far. This phylogenetic tree revealed that C. minuta’s BSH amino acid sequence clusters away from others with a threshold of 70% identity. This is therefore the first description of C. minuta’s BSH protein, which may be involved in its unique role within the human gut microbial ecosystem. MDPI 2021-06-09 /pmc/articles/PMC8228234/ /pubmed/34207623 http://dx.doi.org/10.3390/microorganisms9061252 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Déjean, Guillaume
Tudela, Héloïse
Bruno, Lisa
Kissi, Déborah
Rawadi, Georges
Claus, Sandrine P.
Identifying a Novel Bile Salt Hydrolase from the Keystone Gut Bacterium Christensenella minuta
title Identifying a Novel Bile Salt Hydrolase from the Keystone Gut Bacterium Christensenella minuta
title_full Identifying a Novel Bile Salt Hydrolase from the Keystone Gut Bacterium Christensenella minuta
title_fullStr Identifying a Novel Bile Salt Hydrolase from the Keystone Gut Bacterium Christensenella minuta
title_full_unstemmed Identifying a Novel Bile Salt Hydrolase from the Keystone Gut Bacterium Christensenella minuta
title_short Identifying a Novel Bile Salt Hydrolase from the Keystone Gut Bacterium Christensenella minuta
title_sort identifying a novel bile salt hydrolase from the keystone gut bacterium christensenella minuta
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8228234/
https://www.ncbi.nlm.nih.gov/pubmed/34207623
http://dx.doi.org/10.3390/microorganisms9061252
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