Cargando…

Dynamics and Extent of Non-Structural Protein 1-Antibody Responses in Tick-Borne Encephalitis Vaccination Breakthroughs and Unvaccinated Patients

Tick-borne encephalitis (TBE) has a substantial impact on human public health in many parts of Europe and Asia. Effective inactivated purified whole-virus vaccines are in widespread use in TBE-endemic countries. Nevertheless, vaccination breakthroughs (VBTs) with manifest clinical disease do occur,...

Descripción completa

Detalles Bibliográficos
Autores principales: Stiasny, Karin, Leitner, Agnes, Holzmann, Heidemarie, Heinz, Franz X.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8228328/
https://www.ncbi.nlm.nih.gov/pubmed/34072119
http://dx.doi.org/10.3390/v13061007
_version_ 1783712717471744000
author Stiasny, Karin
Leitner, Agnes
Holzmann, Heidemarie
Heinz, Franz X.
author_facet Stiasny, Karin
Leitner, Agnes
Holzmann, Heidemarie
Heinz, Franz X.
author_sort Stiasny, Karin
collection PubMed
description Tick-borne encephalitis (TBE) has a substantial impact on human public health in many parts of Europe and Asia. Effective inactivated purified whole-virus vaccines are in widespread use in TBE-endemic countries. Nevertheless, vaccination breakthroughs (VBTs) with manifest clinical disease do occur, and their specific serodiagnosis was shown to be facilitated by the detection of antibodies to a non-structural protein (NS1) that is produced during virus replication. However, recent data have shown that NS1 is also present in the current inactivated vaccines, with the potential of inducing corresponding antibodies and obscuring a proper interpretation of NS1-antibody assays for diagnosing VBTs. In our study, we quantified anti-virion and anti-NS1 antibody responses after vaccination as well as after natural infection in TBE patients, both without and with a history of previous TBE vaccination (VBTs). We did not find significant levels of NS1-specific antibodies in serum samples from 48 vaccinees with a completed vaccination schedule. In contrast, all TBE patients mounted an anti-NS1 antibody response, irrespective of whether they were vaccinated or not. Neither the dynamics nor the extent of NS1-antibody formation differed significantly between the two cohorts, arguing against substantial NS1-specific priming and an anamnestic NS1-antibody response in VBTs.
format Online
Article
Text
id pubmed-8228328
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-82283282021-06-26 Dynamics and Extent of Non-Structural Protein 1-Antibody Responses in Tick-Borne Encephalitis Vaccination Breakthroughs and Unvaccinated Patients Stiasny, Karin Leitner, Agnes Holzmann, Heidemarie Heinz, Franz X. Viruses Article Tick-borne encephalitis (TBE) has a substantial impact on human public health in many parts of Europe and Asia. Effective inactivated purified whole-virus vaccines are in widespread use in TBE-endemic countries. Nevertheless, vaccination breakthroughs (VBTs) with manifest clinical disease do occur, and their specific serodiagnosis was shown to be facilitated by the detection of antibodies to a non-structural protein (NS1) that is produced during virus replication. However, recent data have shown that NS1 is also present in the current inactivated vaccines, with the potential of inducing corresponding antibodies and obscuring a proper interpretation of NS1-antibody assays for diagnosing VBTs. In our study, we quantified anti-virion and anti-NS1 antibody responses after vaccination as well as after natural infection in TBE patients, both without and with a history of previous TBE vaccination (VBTs). We did not find significant levels of NS1-specific antibodies in serum samples from 48 vaccinees with a completed vaccination schedule. In contrast, all TBE patients mounted an anti-NS1 antibody response, irrespective of whether they were vaccinated or not. Neither the dynamics nor the extent of NS1-antibody formation differed significantly between the two cohorts, arguing against substantial NS1-specific priming and an anamnestic NS1-antibody response in VBTs. MDPI 2021-05-27 /pmc/articles/PMC8228328/ /pubmed/34072119 http://dx.doi.org/10.3390/v13061007 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Stiasny, Karin
Leitner, Agnes
Holzmann, Heidemarie
Heinz, Franz X.
Dynamics and Extent of Non-Structural Protein 1-Antibody Responses in Tick-Borne Encephalitis Vaccination Breakthroughs and Unvaccinated Patients
title Dynamics and Extent of Non-Structural Protein 1-Antibody Responses in Tick-Borne Encephalitis Vaccination Breakthroughs and Unvaccinated Patients
title_full Dynamics and Extent of Non-Structural Protein 1-Antibody Responses in Tick-Borne Encephalitis Vaccination Breakthroughs and Unvaccinated Patients
title_fullStr Dynamics and Extent of Non-Structural Protein 1-Antibody Responses in Tick-Borne Encephalitis Vaccination Breakthroughs and Unvaccinated Patients
title_full_unstemmed Dynamics and Extent of Non-Structural Protein 1-Antibody Responses in Tick-Borne Encephalitis Vaccination Breakthroughs and Unvaccinated Patients
title_short Dynamics and Extent of Non-Structural Protein 1-Antibody Responses in Tick-Borne Encephalitis Vaccination Breakthroughs and Unvaccinated Patients
title_sort dynamics and extent of non-structural protein 1-antibody responses in tick-borne encephalitis vaccination breakthroughs and unvaccinated patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8228328/
https://www.ncbi.nlm.nih.gov/pubmed/34072119
http://dx.doi.org/10.3390/v13061007
work_keys_str_mv AT stiasnykarin dynamicsandextentofnonstructuralprotein1antibodyresponsesintickborneencephalitisvaccinationbreakthroughsandunvaccinatedpatients
AT leitneragnes dynamicsandextentofnonstructuralprotein1antibodyresponsesintickborneencephalitisvaccinationbreakthroughsandunvaccinatedpatients
AT holzmannheidemarie dynamicsandextentofnonstructuralprotein1antibodyresponsesintickborneencephalitisvaccinationbreakthroughsandunvaccinatedpatients
AT heinzfranzx dynamicsandextentofnonstructuralprotein1antibodyresponsesintickborneencephalitisvaccinationbreakthroughsandunvaccinatedpatients