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Breeding for Virus Resistance and Its Effects on Deformed Wing Virus Infection Patterns in Honey Bee Queens

Viruses, and in particular the deformed wing virus (DWV), are considered as one of the main antagonists of honey bee health. The ‘suppressed in ovo virus infection’ trait (SOV) described for the first time that control of a virus infection can be achieved from genetically inherited traits and that t...

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Autores principales: Bouuaert, David Claeys, De Smet, Lina, de Graaf, Dirk C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8228329/
https://www.ncbi.nlm.nih.gov/pubmed/34199957
http://dx.doi.org/10.3390/v13061074
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author Bouuaert, David Claeys
De Smet, Lina
de Graaf, Dirk C.
author_facet Bouuaert, David Claeys
De Smet, Lina
de Graaf, Dirk C.
author_sort Bouuaert, David Claeys
collection PubMed
description Viruses, and in particular the deformed wing virus (DWV), are considered as one of the main antagonists of honey bee health. The ‘suppressed in ovo virus infection’ trait (SOV) described for the first time that control of a virus infection can be achieved from genetically inherited traits and that the virus state of the eggs is indicative for this. This research aims to explore the effect of the SOV trait on DWV infections in queens descending from both SOV-positive (QDS+) and SOV-negative (QDS–) queens. Twenty QDS+ and QDS– were reared from each time four queens in the same starter–finisher colony. From each queen the head, thorax, ovaries, spermatheca, guts and eviscerated abdomen were dissected and screened for the presence of the DWV-A and DWV-B genotype using qRT-PCR. Queens descending from SOV-positive queens showed significant lower infection loads for DWV-A and DWV-B as well as a lower number of infected tissues for DWV-A. Surprisingly, differences were less expressed in the reproductive tissues, the ovaries and spermatheca. These results confirm that selection on the SOV trait is associated with increased virus resistance across viral genotypes and that this selection drives DWV towards an increased tissue specificity for the reproductive tissues. Further research is needed to explore the mechanisms underlying the interaction between the antiviral response and DWV.
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spelling pubmed-82283292021-06-26 Breeding for Virus Resistance and Its Effects on Deformed Wing Virus Infection Patterns in Honey Bee Queens Bouuaert, David Claeys De Smet, Lina de Graaf, Dirk C. Viruses Article Viruses, and in particular the deformed wing virus (DWV), are considered as one of the main antagonists of honey bee health. The ‘suppressed in ovo virus infection’ trait (SOV) described for the first time that control of a virus infection can be achieved from genetically inherited traits and that the virus state of the eggs is indicative for this. This research aims to explore the effect of the SOV trait on DWV infections in queens descending from both SOV-positive (QDS+) and SOV-negative (QDS–) queens. Twenty QDS+ and QDS– were reared from each time four queens in the same starter–finisher colony. From each queen the head, thorax, ovaries, spermatheca, guts and eviscerated abdomen were dissected and screened for the presence of the DWV-A and DWV-B genotype using qRT-PCR. Queens descending from SOV-positive queens showed significant lower infection loads for DWV-A and DWV-B as well as a lower number of infected tissues for DWV-A. Surprisingly, differences were less expressed in the reproductive tissues, the ovaries and spermatheca. These results confirm that selection on the SOV trait is associated with increased virus resistance across viral genotypes and that this selection drives DWV towards an increased tissue specificity for the reproductive tissues. Further research is needed to explore the mechanisms underlying the interaction between the antiviral response and DWV. MDPI 2021-06-04 /pmc/articles/PMC8228329/ /pubmed/34199957 http://dx.doi.org/10.3390/v13061074 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bouuaert, David Claeys
De Smet, Lina
de Graaf, Dirk C.
Breeding for Virus Resistance and Its Effects on Deformed Wing Virus Infection Patterns in Honey Bee Queens
title Breeding for Virus Resistance and Its Effects on Deformed Wing Virus Infection Patterns in Honey Bee Queens
title_full Breeding for Virus Resistance and Its Effects on Deformed Wing Virus Infection Patterns in Honey Bee Queens
title_fullStr Breeding for Virus Resistance and Its Effects on Deformed Wing Virus Infection Patterns in Honey Bee Queens
title_full_unstemmed Breeding for Virus Resistance and Its Effects on Deformed Wing Virus Infection Patterns in Honey Bee Queens
title_short Breeding for Virus Resistance and Its Effects on Deformed Wing Virus Infection Patterns in Honey Bee Queens
title_sort breeding for virus resistance and its effects on deformed wing virus infection patterns in honey bee queens
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8228329/
https://www.ncbi.nlm.nih.gov/pubmed/34199957
http://dx.doi.org/10.3390/v13061074
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