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Comparative Analysis of the Intermolt and Postmolt Hepatopancreas Transcriptomes Provides Insight into the Mechanisms of Procambarus clarkii Molting Process

In the present study, we used RNA-Seq to investigate the expression changes in the transcriptomes of two molting stages (postmolt (M) and intermolt (NM)) of the red swamp crayfish and identified differentially expressed genes. The transcriptomes of the two molting stages were de novo assembled into ...

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Autores principales: Su, Shengyan, Munganga, Brian Pelekelo, Tian, Can, Li, Jianlin, Yu, Fan, Li, Hongxia, Wang, Meiyao, He, Xinjin, Tang, Yongkai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8228513/
https://www.ncbi.nlm.nih.gov/pubmed/34070595
http://dx.doi.org/10.3390/life11060480
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author Su, Shengyan
Munganga, Brian Pelekelo
Tian, Can
Li, Jianlin
Yu, Fan
Li, Hongxia
Wang, Meiyao
He, Xinjin
Tang, Yongkai
author_facet Su, Shengyan
Munganga, Brian Pelekelo
Tian, Can
Li, Jianlin
Yu, Fan
Li, Hongxia
Wang, Meiyao
He, Xinjin
Tang, Yongkai
author_sort Su, Shengyan
collection PubMed
description In the present study, we used RNA-Seq to investigate the expression changes in the transcriptomes of two molting stages (postmolt (M) and intermolt (NM)) of the red swamp crayfish and identified differentially expressed genes. The transcriptomes of the two molting stages were de novo assembled into 139,100 unigenes with a mean length of 675.59 bp. The results were searched against the NCBI, NR, KEGG, Swissprot, and KOG databases, to annotate gene descriptions, associate them with gene ontology terms, and assign them to pathways. Furthermore, using the DESeq R package, differentially expressed genes were evaluated. The analysis revealed that 2347 genes were significantly (p > 0.05) differentially expressed in the two molting stages. Several genes and other factors involved in several molecular events critical for the molting process, such as energy requirements, hormonal regulation, immune response, and exoskeleton formation were identified and evaluated by correlation and KEGG analysis. The expression profiles of transcripts detected via RNA-Seq were validated by real-time PCR assay of eight genes. The information presented here provides a transient view of the hepatopancreas transcripts available in the postmolt and intermolt stage of crayfish, hormonal regulation, immune response, and skeletal-related activities during the postmolt stage and the intermolt stage.
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spelling pubmed-82285132021-06-26 Comparative Analysis of the Intermolt and Postmolt Hepatopancreas Transcriptomes Provides Insight into the Mechanisms of Procambarus clarkii Molting Process Su, Shengyan Munganga, Brian Pelekelo Tian, Can Li, Jianlin Yu, Fan Li, Hongxia Wang, Meiyao He, Xinjin Tang, Yongkai Life (Basel) Article In the present study, we used RNA-Seq to investigate the expression changes in the transcriptomes of two molting stages (postmolt (M) and intermolt (NM)) of the red swamp crayfish and identified differentially expressed genes. The transcriptomes of the two molting stages were de novo assembled into 139,100 unigenes with a mean length of 675.59 bp. The results were searched against the NCBI, NR, KEGG, Swissprot, and KOG databases, to annotate gene descriptions, associate them with gene ontology terms, and assign them to pathways. Furthermore, using the DESeq R package, differentially expressed genes were evaluated. The analysis revealed that 2347 genes were significantly (p > 0.05) differentially expressed in the two molting stages. Several genes and other factors involved in several molecular events critical for the molting process, such as energy requirements, hormonal regulation, immune response, and exoskeleton formation were identified and evaluated by correlation and KEGG analysis. The expression profiles of transcripts detected via RNA-Seq were validated by real-time PCR assay of eight genes. The information presented here provides a transient view of the hepatopancreas transcripts available in the postmolt and intermolt stage of crayfish, hormonal regulation, immune response, and skeletal-related activities during the postmolt stage and the intermolt stage. MDPI 2021-05-25 /pmc/articles/PMC8228513/ /pubmed/34070595 http://dx.doi.org/10.3390/life11060480 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Su, Shengyan
Munganga, Brian Pelekelo
Tian, Can
Li, Jianlin
Yu, Fan
Li, Hongxia
Wang, Meiyao
He, Xinjin
Tang, Yongkai
Comparative Analysis of the Intermolt and Postmolt Hepatopancreas Transcriptomes Provides Insight into the Mechanisms of Procambarus clarkii Molting Process
title Comparative Analysis of the Intermolt and Postmolt Hepatopancreas Transcriptomes Provides Insight into the Mechanisms of Procambarus clarkii Molting Process
title_full Comparative Analysis of the Intermolt and Postmolt Hepatopancreas Transcriptomes Provides Insight into the Mechanisms of Procambarus clarkii Molting Process
title_fullStr Comparative Analysis of the Intermolt and Postmolt Hepatopancreas Transcriptomes Provides Insight into the Mechanisms of Procambarus clarkii Molting Process
title_full_unstemmed Comparative Analysis of the Intermolt and Postmolt Hepatopancreas Transcriptomes Provides Insight into the Mechanisms of Procambarus clarkii Molting Process
title_short Comparative Analysis of the Intermolt and Postmolt Hepatopancreas Transcriptomes Provides Insight into the Mechanisms of Procambarus clarkii Molting Process
title_sort comparative analysis of the intermolt and postmolt hepatopancreas transcriptomes provides insight into the mechanisms of procambarus clarkii molting process
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8228513/
https://www.ncbi.nlm.nih.gov/pubmed/34070595
http://dx.doi.org/10.3390/life11060480
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