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Reovirus Low-Density Particles Package Cellular RNA

Packaging of segmented, double-stranded RNA viral genomes requires coordination of viral proteins and RNA segments. For mammalian orthoreovirus (reovirus), evidence suggests either all ten or zero viral RNA segments are simultaneously packaged in a highly coordinated process hypothesized to exclude...

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Autores principales: Thoner, Timothy W., Ye, Xiang, Karijolich, John, Ogden, Kristen M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8228547/
https://www.ncbi.nlm.nih.gov/pubmed/34201386
http://dx.doi.org/10.3390/v13061096
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author Thoner, Timothy W.
Ye, Xiang
Karijolich, John
Ogden, Kristen M.
author_facet Thoner, Timothy W.
Ye, Xiang
Karijolich, John
Ogden, Kristen M.
author_sort Thoner, Timothy W.
collection PubMed
description Packaging of segmented, double-stranded RNA viral genomes requires coordination of viral proteins and RNA segments. For mammalian orthoreovirus (reovirus), evidence suggests either all ten or zero viral RNA segments are simultaneously packaged in a highly coordinated process hypothesized to exclude host RNA. Accordingly, reovirus generates genome-containing virions and “genomeless” top component particles. Whether reovirus virions or top component particles package host RNA is unknown. To gain insight into reovirus packaging potential and mechanisms, we employed next-generation RNA-sequencing to define the RNA content of enriched reovirus particles. Reovirus virions exclusively packaged viral double-stranded RNA. In contrast, reovirus top component particles contained similar proportions but reduced amounts of viral double-stranded RNA and were selectively enriched for numerous host RNA species, especially short, non-polyadenylated transcripts. Host RNA selection was not dependent on RNA abundance in the cell, and specifically enriched host RNAs varied for two reovirus strains and were not selected solely by the viral RNA polymerase. Collectively, these findings indicate that genome packaging into reovirus virions is exquisitely selective, while incorporation of host RNAs into top component particles is differentially selective and may contribute to or result from inefficient viral RNA packaging.
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spelling pubmed-82285472021-06-26 Reovirus Low-Density Particles Package Cellular RNA Thoner, Timothy W. Ye, Xiang Karijolich, John Ogden, Kristen M. Viruses Article Packaging of segmented, double-stranded RNA viral genomes requires coordination of viral proteins and RNA segments. For mammalian orthoreovirus (reovirus), evidence suggests either all ten or zero viral RNA segments are simultaneously packaged in a highly coordinated process hypothesized to exclude host RNA. Accordingly, reovirus generates genome-containing virions and “genomeless” top component particles. Whether reovirus virions or top component particles package host RNA is unknown. To gain insight into reovirus packaging potential and mechanisms, we employed next-generation RNA-sequencing to define the RNA content of enriched reovirus particles. Reovirus virions exclusively packaged viral double-stranded RNA. In contrast, reovirus top component particles contained similar proportions but reduced amounts of viral double-stranded RNA and were selectively enriched for numerous host RNA species, especially short, non-polyadenylated transcripts. Host RNA selection was not dependent on RNA abundance in the cell, and specifically enriched host RNAs varied for two reovirus strains and were not selected solely by the viral RNA polymerase. Collectively, these findings indicate that genome packaging into reovirus virions is exquisitely selective, while incorporation of host RNAs into top component particles is differentially selective and may contribute to or result from inefficient viral RNA packaging. MDPI 2021-06-08 /pmc/articles/PMC8228547/ /pubmed/34201386 http://dx.doi.org/10.3390/v13061096 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Thoner, Timothy W.
Ye, Xiang
Karijolich, John
Ogden, Kristen M.
Reovirus Low-Density Particles Package Cellular RNA
title Reovirus Low-Density Particles Package Cellular RNA
title_full Reovirus Low-Density Particles Package Cellular RNA
title_fullStr Reovirus Low-Density Particles Package Cellular RNA
title_full_unstemmed Reovirus Low-Density Particles Package Cellular RNA
title_short Reovirus Low-Density Particles Package Cellular RNA
title_sort reovirus low-density particles package cellular rna
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8228547/
https://www.ncbi.nlm.nih.gov/pubmed/34201386
http://dx.doi.org/10.3390/v13061096
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