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Topical Gynura procumbens as a Novel Therapeutic Improves Wound Healing in Diabetic Mice

Nonhealing wounds are major socioeconomic challenges to healthcare systems worldwide. Therefore, there is a substantially unmet need to develop new drugs for wound healing. Gynura procumbens, a herb found in Southeast Asia, may be an effective therapeutic for nonhealing diabetic wounds. The aim of t...

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Autores principales: Sutthammikorn, Nutda, Supajatura, Volaluck, Yue, Hainan, Takahashi, Miho, Chansakaow, Sunee, Nakano, Nobuhiro, Song, Pu, Ogawa, Takasuke, Ikeda, Shigaku, Okumura, Ko, Ogawa, Hideoki, Niyonsaba, François
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8228548/
https://www.ncbi.nlm.nih.gov/pubmed/34205899
http://dx.doi.org/10.3390/plants10061122
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author Sutthammikorn, Nutda
Supajatura, Volaluck
Yue, Hainan
Takahashi, Miho
Chansakaow, Sunee
Nakano, Nobuhiro
Song, Pu
Ogawa, Takasuke
Ikeda, Shigaku
Okumura, Ko
Ogawa, Hideoki
Niyonsaba, François
author_facet Sutthammikorn, Nutda
Supajatura, Volaluck
Yue, Hainan
Takahashi, Miho
Chansakaow, Sunee
Nakano, Nobuhiro
Song, Pu
Ogawa, Takasuke
Ikeda, Shigaku
Okumura, Ko
Ogawa, Hideoki
Niyonsaba, François
author_sort Sutthammikorn, Nutda
collection PubMed
description Nonhealing wounds are major socioeconomic challenges to healthcare systems worldwide. Therefore, there is a substantially unmet need to develop new drugs for wound healing. Gynura procumbens, a herb found in Southeast Asia, may be an effective therapeutic for nonhealing diabetic wounds. The aim of this study was to evaluate the efficacy of G. procumbens on wound healing in the diabetic milieu. G. procumbens extract was obtained using 95% ethanol and its components were determined by thin layer chromatography. Diabetes was induced in mice using streptozotocin. We found that G. procumbens extract contained stigmasterol, kaempferol and quercetin compounds. Topical application of G. procumbens on the wounded skin of diabetic mice accelerated wound healing and induced the expression of angiogenin, epidermal growth factor, fibroblast growth factor, transforming growth factor and vascular endothelial growth factor. Furthermore, G. procumbens promoted in vitro wound healing and enhanced the migration and/or proliferation of human endothelial cells, fibroblasts, keratinocytes and mast cells cultured in diabetic conditions. Finally, G. procumbens promoted vascular formation in the diabetic mice. To the best of our knowledge, this is the first study that evaluates in vivo wound healing activities of G. procumbens and activation of cells involved in wound healing process in diabetic conditions. The findings that G. procumbens accelerates wound healing and activates cells involved in the wound healing process suggest that G. procumbens might be an effective alternative therapeutic option for nonhealing diabetic wounds.
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spelling pubmed-82285482021-06-26 Topical Gynura procumbens as a Novel Therapeutic Improves Wound Healing in Diabetic Mice Sutthammikorn, Nutda Supajatura, Volaluck Yue, Hainan Takahashi, Miho Chansakaow, Sunee Nakano, Nobuhiro Song, Pu Ogawa, Takasuke Ikeda, Shigaku Okumura, Ko Ogawa, Hideoki Niyonsaba, François Plants (Basel) Article Nonhealing wounds are major socioeconomic challenges to healthcare systems worldwide. Therefore, there is a substantially unmet need to develop new drugs for wound healing. Gynura procumbens, a herb found in Southeast Asia, may be an effective therapeutic for nonhealing diabetic wounds. The aim of this study was to evaluate the efficacy of G. procumbens on wound healing in the diabetic milieu. G. procumbens extract was obtained using 95% ethanol and its components were determined by thin layer chromatography. Diabetes was induced in mice using streptozotocin. We found that G. procumbens extract contained stigmasterol, kaempferol and quercetin compounds. Topical application of G. procumbens on the wounded skin of diabetic mice accelerated wound healing and induced the expression of angiogenin, epidermal growth factor, fibroblast growth factor, transforming growth factor and vascular endothelial growth factor. Furthermore, G. procumbens promoted in vitro wound healing and enhanced the migration and/or proliferation of human endothelial cells, fibroblasts, keratinocytes and mast cells cultured in diabetic conditions. Finally, G. procumbens promoted vascular formation in the diabetic mice. To the best of our knowledge, this is the first study that evaluates in vivo wound healing activities of G. procumbens and activation of cells involved in wound healing process in diabetic conditions. The findings that G. procumbens accelerates wound healing and activates cells involved in the wound healing process suggest that G. procumbens might be an effective alternative therapeutic option for nonhealing diabetic wounds. MDPI 2021-06-01 /pmc/articles/PMC8228548/ /pubmed/34205899 http://dx.doi.org/10.3390/plants10061122 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sutthammikorn, Nutda
Supajatura, Volaluck
Yue, Hainan
Takahashi, Miho
Chansakaow, Sunee
Nakano, Nobuhiro
Song, Pu
Ogawa, Takasuke
Ikeda, Shigaku
Okumura, Ko
Ogawa, Hideoki
Niyonsaba, François
Topical Gynura procumbens as a Novel Therapeutic Improves Wound Healing in Diabetic Mice
title Topical Gynura procumbens as a Novel Therapeutic Improves Wound Healing in Diabetic Mice
title_full Topical Gynura procumbens as a Novel Therapeutic Improves Wound Healing in Diabetic Mice
title_fullStr Topical Gynura procumbens as a Novel Therapeutic Improves Wound Healing in Diabetic Mice
title_full_unstemmed Topical Gynura procumbens as a Novel Therapeutic Improves Wound Healing in Diabetic Mice
title_short Topical Gynura procumbens as a Novel Therapeutic Improves Wound Healing in Diabetic Mice
title_sort topical gynura procumbens as a novel therapeutic improves wound healing in diabetic mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8228548/
https://www.ncbi.nlm.nih.gov/pubmed/34205899
http://dx.doi.org/10.3390/plants10061122
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