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Topical Gynura procumbens as a Novel Therapeutic Improves Wound Healing in Diabetic Mice
Nonhealing wounds are major socioeconomic challenges to healthcare systems worldwide. Therefore, there is a substantially unmet need to develop new drugs for wound healing. Gynura procumbens, a herb found in Southeast Asia, may be an effective therapeutic for nonhealing diabetic wounds. The aim of t...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8228548/ https://www.ncbi.nlm.nih.gov/pubmed/34205899 http://dx.doi.org/10.3390/plants10061122 |
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author | Sutthammikorn, Nutda Supajatura, Volaluck Yue, Hainan Takahashi, Miho Chansakaow, Sunee Nakano, Nobuhiro Song, Pu Ogawa, Takasuke Ikeda, Shigaku Okumura, Ko Ogawa, Hideoki Niyonsaba, François |
author_facet | Sutthammikorn, Nutda Supajatura, Volaluck Yue, Hainan Takahashi, Miho Chansakaow, Sunee Nakano, Nobuhiro Song, Pu Ogawa, Takasuke Ikeda, Shigaku Okumura, Ko Ogawa, Hideoki Niyonsaba, François |
author_sort | Sutthammikorn, Nutda |
collection | PubMed |
description | Nonhealing wounds are major socioeconomic challenges to healthcare systems worldwide. Therefore, there is a substantially unmet need to develop new drugs for wound healing. Gynura procumbens, a herb found in Southeast Asia, may be an effective therapeutic for nonhealing diabetic wounds. The aim of this study was to evaluate the efficacy of G. procumbens on wound healing in the diabetic milieu. G. procumbens extract was obtained using 95% ethanol and its components were determined by thin layer chromatography. Diabetes was induced in mice using streptozotocin. We found that G. procumbens extract contained stigmasterol, kaempferol and quercetin compounds. Topical application of G. procumbens on the wounded skin of diabetic mice accelerated wound healing and induced the expression of angiogenin, epidermal growth factor, fibroblast growth factor, transforming growth factor and vascular endothelial growth factor. Furthermore, G. procumbens promoted in vitro wound healing and enhanced the migration and/or proliferation of human endothelial cells, fibroblasts, keratinocytes and mast cells cultured in diabetic conditions. Finally, G. procumbens promoted vascular formation in the diabetic mice. To the best of our knowledge, this is the first study that evaluates in vivo wound healing activities of G. procumbens and activation of cells involved in wound healing process in diabetic conditions. The findings that G. procumbens accelerates wound healing and activates cells involved in the wound healing process suggest that G. procumbens might be an effective alternative therapeutic option for nonhealing diabetic wounds. |
format | Online Article Text |
id | pubmed-8228548 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-82285482021-06-26 Topical Gynura procumbens as a Novel Therapeutic Improves Wound Healing in Diabetic Mice Sutthammikorn, Nutda Supajatura, Volaluck Yue, Hainan Takahashi, Miho Chansakaow, Sunee Nakano, Nobuhiro Song, Pu Ogawa, Takasuke Ikeda, Shigaku Okumura, Ko Ogawa, Hideoki Niyonsaba, François Plants (Basel) Article Nonhealing wounds are major socioeconomic challenges to healthcare systems worldwide. Therefore, there is a substantially unmet need to develop new drugs for wound healing. Gynura procumbens, a herb found in Southeast Asia, may be an effective therapeutic for nonhealing diabetic wounds. The aim of this study was to evaluate the efficacy of G. procumbens on wound healing in the diabetic milieu. G. procumbens extract was obtained using 95% ethanol and its components were determined by thin layer chromatography. Diabetes was induced in mice using streptozotocin. We found that G. procumbens extract contained stigmasterol, kaempferol and quercetin compounds. Topical application of G. procumbens on the wounded skin of diabetic mice accelerated wound healing and induced the expression of angiogenin, epidermal growth factor, fibroblast growth factor, transforming growth factor and vascular endothelial growth factor. Furthermore, G. procumbens promoted in vitro wound healing and enhanced the migration and/or proliferation of human endothelial cells, fibroblasts, keratinocytes and mast cells cultured in diabetic conditions. Finally, G. procumbens promoted vascular formation in the diabetic mice. To the best of our knowledge, this is the first study that evaluates in vivo wound healing activities of G. procumbens and activation of cells involved in wound healing process in diabetic conditions. The findings that G. procumbens accelerates wound healing and activates cells involved in the wound healing process suggest that G. procumbens might be an effective alternative therapeutic option for nonhealing diabetic wounds. MDPI 2021-06-01 /pmc/articles/PMC8228548/ /pubmed/34205899 http://dx.doi.org/10.3390/plants10061122 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sutthammikorn, Nutda Supajatura, Volaluck Yue, Hainan Takahashi, Miho Chansakaow, Sunee Nakano, Nobuhiro Song, Pu Ogawa, Takasuke Ikeda, Shigaku Okumura, Ko Ogawa, Hideoki Niyonsaba, François Topical Gynura procumbens as a Novel Therapeutic Improves Wound Healing in Diabetic Mice |
title | Topical Gynura procumbens as a Novel Therapeutic Improves Wound Healing in Diabetic Mice |
title_full | Topical Gynura procumbens as a Novel Therapeutic Improves Wound Healing in Diabetic Mice |
title_fullStr | Topical Gynura procumbens as a Novel Therapeutic Improves Wound Healing in Diabetic Mice |
title_full_unstemmed | Topical Gynura procumbens as a Novel Therapeutic Improves Wound Healing in Diabetic Mice |
title_short | Topical Gynura procumbens as a Novel Therapeutic Improves Wound Healing in Diabetic Mice |
title_sort | topical gynura procumbens as a novel therapeutic improves wound healing in diabetic mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8228548/ https://www.ncbi.nlm.nih.gov/pubmed/34205899 http://dx.doi.org/10.3390/plants10061122 |
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