Cargando…
Simple Determination of Gold Nanocrystal Dimensions by Analytical Ultracentrifugation via Surface Ligand-Solvent Density Matching
Analytical ultracentrifugation (AUC) is a powerful technique to observe colloidal nanocrystals (NCs) directly in solution and obtain critical information about their physical-chemical properties. Nevertheless, a more comprehensive implementation of AUC for the characterisation of such a class of cry...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8228700/ https://www.ncbi.nlm.nih.gov/pubmed/34071534 http://dx.doi.org/10.3390/nano11061427 |
_version_ | 1783712803697197056 |
---|---|
author | González-Rubio, Guillermo Hilbert, Holger Rosenberg, Rose Ni, Bing Fuhrer, Lisa Cölfen, Helmut |
author_facet | González-Rubio, Guillermo Hilbert, Holger Rosenberg, Rose Ni, Bing Fuhrer, Lisa Cölfen, Helmut |
author_sort | González-Rubio, Guillermo |
collection | PubMed |
description | Analytical ultracentrifugation (AUC) is a powerful technique to observe colloidal nanocrystals (NCs) directly in solution and obtain critical information about their physical-chemical properties. Nevertheless, a more comprehensive implementation of AUC for the characterisation of such a class of crystalline colloids has been traditionally impaired by the requirement of having a priori knowledge of the complex, multilayered structure formed by NC in solution. This includes the nature (density and mass) of the surface ligands (SLs) that provide NC colloidal stability and the shell of solvent molecules formed on it. Herein, we propose a methodology to determine the NCs size by using SLs with a density equal to that of the solvent. Thereby, the buoyancy force of the SL shell is neutral, and the density of the NCs is sufficient a priori knowledge to calculate their related mass and size distributions. The simplicity and reliability of the method are evaluated with cetyltrimethylammonium bromide (CTAB) stabilized spherical gold NCs (AuNCs) of dimensions ranging from 1 to 17 nm. The proposed method has great potential to be transferred to any non-crystalline and crystalline colloids of different nature and composition, which have a density that is equal to the bulk and can be stabilized by SLs having a density that matches that of the solvent. |
format | Online Article Text |
id | pubmed-8228700 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-82287002021-06-26 Simple Determination of Gold Nanocrystal Dimensions by Analytical Ultracentrifugation via Surface Ligand-Solvent Density Matching González-Rubio, Guillermo Hilbert, Holger Rosenberg, Rose Ni, Bing Fuhrer, Lisa Cölfen, Helmut Nanomaterials (Basel) Article Analytical ultracentrifugation (AUC) is a powerful technique to observe colloidal nanocrystals (NCs) directly in solution and obtain critical information about their physical-chemical properties. Nevertheless, a more comprehensive implementation of AUC for the characterisation of such a class of crystalline colloids has been traditionally impaired by the requirement of having a priori knowledge of the complex, multilayered structure formed by NC in solution. This includes the nature (density and mass) of the surface ligands (SLs) that provide NC colloidal stability and the shell of solvent molecules formed on it. Herein, we propose a methodology to determine the NCs size by using SLs with a density equal to that of the solvent. Thereby, the buoyancy force of the SL shell is neutral, and the density of the NCs is sufficient a priori knowledge to calculate their related mass and size distributions. The simplicity and reliability of the method are evaluated with cetyltrimethylammonium bromide (CTAB) stabilized spherical gold NCs (AuNCs) of dimensions ranging from 1 to 17 nm. The proposed method has great potential to be transferred to any non-crystalline and crystalline colloids of different nature and composition, which have a density that is equal to the bulk and can be stabilized by SLs having a density that matches that of the solvent. MDPI 2021-05-28 /pmc/articles/PMC8228700/ /pubmed/34071534 http://dx.doi.org/10.3390/nano11061427 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article González-Rubio, Guillermo Hilbert, Holger Rosenberg, Rose Ni, Bing Fuhrer, Lisa Cölfen, Helmut Simple Determination of Gold Nanocrystal Dimensions by Analytical Ultracentrifugation via Surface Ligand-Solvent Density Matching |
title | Simple Determination of Gold Nanocrystal Dimensions by Analytical Ultracentrifugation via Surface Ligand-Solvent Density Matching |
title_full | Simple Determination of Gold Nanocrystal Dimensions by Analytical Ultracentrifugation via Surface Ligand-Solvent Density Matching |
title_fullStr | Simple Determination of Gold Nanocrystal Dimensions by Analytical Ultracentrifugation via Surface Ligand-Solvent Density Matching |
title_full_unstemmed | Simple Determination of Gold Nanocrystal Dimensions by Analytical Ultracentrifugation via Surface Ligand-Solvent Density Matching |
title_short | Simple Determination of Gold Nanocrystal Dimensions by Analytical Ultracentrifugation via Surface Ligand-Solvent Density Matching |
title_sort | simple determination of gold nanocrystal dimensions by analytical ultracentrifugation via surface ligand-solvent density matching |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8228700/ https://www.ncbi.nlm.nih.gov/pubmed/34071534 http://dx.doi.org/10.3390/nano11061427 |
work_keys_str_mv | AT gonzalezrubioguillermo simpledeterminationofgoldnanocrystaldimensionsbyanalyticalultracentrifugationviasurfaceligandsolventdensitymatching AT hilbertholger simpledeterminationofgoldnanocrystaldimensionsbyanalyticalultracentrifugationviasurfaceligandsolventdensitymatching AT rosenbergrose simpledeterminationofgoldnanocrystaldimensionsbyanalyticalultracentrifugationviasurfaceligandsolventdensitymatching AT nibing simpledeterminationofgoldnanocrystaldimensionsbyanalyticalultracentrifugationviasurfaceligandsolventdensitymatching AT fuhrerlisa simpledeterminationofgoldnanocrystaldimensionsbyanalyticalultracentrifugationviasurfaceligandsolventdensitymatching AT colfenhelmut simpledeterminationofgoldnanocrystaldimensionsbyanalyticalultracentrifugationviasurfaceligandsolventdensitymatching |