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Ultrabright Fluorescent Silica Nanoparticles for Dual pH and Temperature Measurements

The mesoporous nature of silica nanoparticles provides a novel platform for the development of ultrabright fluorescent particles, which have organic molecular fluorescent dyes physically encapsulated inside the silica pores. The close proximity of the dye molecules, which is possible without fluores...

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Detalles Bibliográficos
Autores principales: Peerzade, Saquib Ahmed M. A., Makarova, Nadezhda, Sokolov, Igor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8228773/
https://www.ncbi.nlm.nih.gov/pubmed/34207605
http://dx.doi.org/10.3390/nano11061524
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author Peerzade, Saquib Ahmed M. A.
Makarova, Nadezhda
Sokolov, Igor
author_facet Peerzade, Saquib Ahmed M. A.
Makarova, Nadezhda
Sokolov, Igor
author_sort Peerzade, Saquib Ahmed M. A.
collection PubMed
description The mesoporous nature of silica nanoparticles provides a novel platform for the development of ultrabright fluorescent particles, which have organic molecular fluorescent dyes physically encapsulated inside the silica pores. The close proximity of the dye molecules, which is possible without fluorescence quenching, gives an advantage of building sensors using FRET coupling between the encapsulated dye molecules. Here we present the use of this approach to demonstrate the assembly of ultrabright fluorescent ratiometric sensors capable of simultaneous acidity (pH) and temperature measurements. FRET pairs of the temperature-responsive, pH-sensitive and reference dyes are physically encapsulated inside the silica matrix of ~50 nm particles. We demonstrate that the particles can be used to measure both the temperature in the biologically relevant range (20 to 50 °C) and pH within 4 to 7 range with the error (mean absolute deviation) of 0.54 °C and 0.09, respectively. Stability of the sensor is demonstrated. The sensitivity of the sensor ranges within 0.2–3% °C(−1) for the measurements of temperature and 2–6% pH(−1) for acidity.
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spelling pubmed-82287732021-06-26 Ultrabright Fluorescent Silica Nanoparticles for Dual pH and Temperature Measurements Peerzade, Saquib Ahmed M. A. Makarova, Nadezhda Sokolov, Igor Nanomaterials (Basel) Article The mesoporous nature of silica nanoparticles provides a novel platform for the development of ultrabright fluorescent particles, which have organic molecular fluorescent dyes physically encapsulated inside the silica pores. The close proximity of the dye molecules, which is possible without fluorescence quenching, gives an advantage of building sensors using FRET coupling between the encapsulated dye molecules. Here we present the use of this approach to demonstrate the assembly of ultrabright fluorescent ratiometric sensors capable of simultaneous acidity (pH) and temperature measurements. FRET pairs of the temperature-responsive, pH-sensitive and reference dyes are physically encapsulated inside the silica matrix of ~50 nm particles. We demonstrate that the particles can be used to measure both the temperature in the biologically relevant range (20 to 50 °C) and pH within 4 to 7 range with the error (mean absolute deviation) of 0.54 °C and 0.09, respectively. Stability of the sensor is demonstrated. The sensitivity of the sensor ranges within 0.2–3% °C(−1) for the measurements of temperature and 2–6% pH(−1) for acidity. MDPI 2021-06-09 /pmc/articles/PMC8228773/ /pubmed/34207605 http://dx.doi.org/10.3390/nano11061524 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Peerzade, Saquib Ahmed M. A.
Makarova, Nadezhda
Sokolov, Igor
Ultrabright Fluorescent Silica Nanoparticles for Dual pH and Temperature Measurements
title Ultrabright Fluorescent Silica Nanoparticles for Dual pH and Temperature Measurements
title_full Ultrabright Fluorescent Silica Nanoparticles for Dual pH and Temperature Measurements
title_fullStr Ultrabright Fluorescent Silica Nanoparticles for Dual pH and Temperature Measurements
title_full_unstemmed Ultrabright Fluorescent Silica Nanoparticles for Dual pH and Temperature Measurements
title_short Ultrabright Fluorescent Silica Nanoparticles for Dual pH and Temperature Measurements
title_sort ultrabright fluorescent silica nanoparticles for dual ph and temperature measurements
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8228773/
https://www.ncbi.nlm.nih.gov/pubmed/34207605
http://dx.doi.org/10.3390/nano11061524
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