The Challenge of Illusory Perception of Animals: The Impact of Methodological Variability in Cross-Species Investigation

SIMPLE SUMMARY: Research in neurobiology and ethology has given us a glimpse into the different perceptual worlds of animals. More recently, visual illusions have been used in behavioural research to compare the perception between different animal species. The studies conducted so far have provided contradictory results, raising the possibility that different methodological approaches might influence illusory perception. Here, we review the literature on this topic, considering both field and laboratory studies. In addition, we compare the two approaches used in laboratories, namely spontaneous choice tests and training procedures, highlighting both their relevance and their potential weaknesses. Adopting both procedures has the potential to combine their advantages. Although this twofold approach has seldomly been adopted, we expect it will become more widely used in the near future in order to shed light on the heterogeneous pattern observed in the literature of visual illusions. ABSTRACT: Although we live on the same planet, there are countless different ways of seeing the surroundings that reflect the different individual experiences and selective pressures. In recent decades, visual illusions have been used in behavioural research to compare the perception between different vertebrate species. The studies conducted so far have provided contradictory results, suggesting that the underlying perceptual mechanisms may differ across species. Besides the differentiation of the perceptual mechanisms, another explanation could be taken into account. Indeed, the different studies often used different methodologies that could have potentially introduced confounding factors. In fact, the possibility exists that the illusory perception is influenced by the different methodologies and the test design. Almost every study of this research field has been conducted in laboratories adopting two different methodological approaches: a spontaneous choice test or a training procedure. In the spontaneous choice test, a subject is presented with biologically relevant stimuli in an illusory context, whereas, in the training procedure, a subject has to undergo an extensive training during which neutral stimuli are associated with a biologically relevant reward. Here, we review the literature on this topic, highlighting both the relevance and the potential weaknesses of the different methodological approaches.