Cargando…
The neuroprotective effects of AMN082 on neuronal apoptosis in rats after traumatic brain injury
BACKGROUND: The aim of this study was to investigate whether AMN082 exerts its neuroprotective effect by attenuating glutamate receptor-associated neuronal apoptosis and improving functional outcomes after traumatic brain injury (TBI). METHODS: Anesthetized male Sprague–Dawley rats were divided into...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8228939/ https://www.ncbi.nlm.nih.gov/pubmed/34171999 http://dx.doi.org/10.1186/s12868-021-00649-w |
_version_ | 1783712859516043264 |
---|---|
author | Lu, Chung-Che Nyam, Tee-Tau Eric Kuo, Jinn-Rung Lee, Yao-Lin Chio, Chung-Ching Wang, Che-Chuan |
author_facet | Lu, Chung-Che Nyam, Tee-Tau Eric Kuo, Jinn-Rung Lee, Yao-Lin Chio, Chung-Ching Wang, Che-Chuan |
author_sort | Lu, Chung-Che |
collection | PubMed |
description | BACKGROUND: The aim of this study was to investigate whether AMN082 exerts its neuroprotective effect by attenuating glutamate receptor-associated neuronal apoptosis and improving functional outcomes after traumatic brain injury (TBI). METHODS: Anesthetized male Sprague–Dawley rats were divided into the sham-operated, TBI + vehicle, and TBI + AMN082 groups. AMN082 (10 mg/kg) was intraperitoneally injected 0, 24, or 48 h after TBI. In the 120 min after TBI, heart rate, mean arterial pressure, intracranial pressure (ICP), and cerebral perfusion pressure (CPP) were continuously measured. Motor function, the infarct volume, neuronal nitrosative stress-associated apoptosis, and N-methyl-d-aspartate receptor 2A (NR2A) and NR2B expression in the pericontusional cortex were measured on the 3rd day after TBI. RESULTS: The results showed that the AMN082-treated group had a lower ICP and higher CPP after TBI. TBI-induced motor deficits, the increase in infarct volume, neuronal apoptosis, and 3-nitrotyrosine and inducible nitric oxide synthase expression in the pericontusional cortex were significantly improved by AMN082 therapy. Simultaneously, AMN082 increased NR2A and NR2B expression in neuronal cells. CONCLUSIONS: We concluded that intraperitoneal injection of AMN082 for 3 days may ameliorate TBI by attenuating glutamate receptor-associated nitrosative stress and neuronal apoptosis in the pericontusional cortex. We suggest that AMN082 administration in the acute stage may be a promising strategy for TBI. |
format | Online Article Text |
id | pubmed-8228939 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-82289392021-06-28 The neuroprotective effects of AMN082 on neuronal apoptosis in rats after traumatic brain injury Lu, Chung-Che Nyam, Tee-Tau Eric Kuo, Jinn-Rung Lee, Yao-Lin Chio, Chung-Ching Wang, Che-Chuan BMC Neurosci Research BACKGROUND: The aim of this study was to investigate whether AMN082 exerts its neuroprotective effect by attenuating glutamate receptor-associated neuronal apoptosis and improving functional outcomes after traumatic brain injury (TBI). METHODS: Anesthetized male Sprague–Dawley rats were divided into the sham-operated, TBI + vehicle, and TBI + AMN082 groups. AMN082 (10 mg/kg) was intraperitoneally injected 0, 24, or 48 h after TBI. In the 120 min after TBI, heart rate, mean arterial pressure, intracranial pressure (ICP), and cerebral perfusion pressure (CPP) were continuously measured. Motor function, the infarct volume, neuronal nitrosative stress-associated apoptosis, and N-methyl-d-aspartate receptor 2A (NR2A) and NR2B expression in the pericontusional cortex were measured on the 3rd day after TBI. RESULTS: The results showed that the AMN082-treated group had a lower ICP and higher CPP after TBI. TBI-induced motor deficits, the increase in infarct volume, neuronal apoptosis, and 3-nitrotyrosine and inducible nitric oxide synthase expression in the pericontusional cortex were significantly improved by AMN082 therapy. Simultaneously, AMN082 increased NR2A and NR2B expression in neuronal cells. CONCLUSIONS: We concluded that intraperitoneal injection of AMN082 for 3 days may ameliorate TBI by attenuating glutamate receptor-associated nitrosative stress and neuronal apoptosis in the pericontusional cortex. We suggest that AMN082 administration in the acute stage may be a promising strategy for TBI. BioMed Central 2021-06-25 /pmc/articles/PMC8228939/ /pubmed/34171999 http://dx.doi.org/10.1186/s12868-021-00649-w Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Lu, Chung-Che Nyam, Tee-Tau Eric Kuo, Jinn-Rung Lee, Yao-Lin Chio, Chung-Ching Wang, Che-Chuan The neuroprotective effects of AMN082 on neuronal apoptosis in rats after traumatic brain injury |
title | The neuroprotective effects of AMN082 on neuronal apoptosis in rats after traumatic brain injury |
title_full | The neuroprotective effects of AMN082 on neuronal apoptosis in rats after traumatic brain injury |
title_fullStr | The neuroprotective effects of AMN082 on neuronal apoptosis in rats after traumatic brain injury |
title_full_unstemmed | The neuroprotective effects of AMN082 on neuronal apoptosis in rats after traumatic brain injury |
title_short | The neuroprotective effects of AMN082 on neuronal apoptosis in rats after traumatic brain injury |
title_sort | neuroprotective effects of amn082 on neuronal apoptosis in rats after traumatic brain injury |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8228939/ https://www.ncbi.nlm.nih.gov/pubmed/34171999 http://dx.doi.org/10.1186/s12868-021-00649-w |
work_keys_str_mv | AT luchungche theneuroprotectiveeffectsofamn082onneuronalapoptosisinratsaftertraumaticbraininjury AT nyamteetaueric theneuroprotectiveeffectsofamn082onneuronalapoptosisinratsaftertraumaticbraininjury AT kuojinnrung theneuroprotectiveeffectsofamn082onneuronalapoptosisinratsaftertraumaticbraininjury AT leeyaolin theneuroprotectiveeffectsofamn082onneuronalapoptosisinratsaftertraumaticbraininjury AT chiochungching theneuroprotectiveeffectsofamn082onneuronalapoptosisinratsaftertraumaticbraininjury AT wangchechuan theneuroprotectiveeffectsofamn082onneuronalapoptosisinratsaftertraumaticbraininjury AT luchungche neuroprotectiveeffectsofamn082onneuronalapoptosisinratsaftertraumaticbraininjury AT nyamteetaueric neuroprotectiveeffectsofamn082onneuronalapoptosisinratsaftertraumaticbraininjury AT kuojinnrung neuroprotectiveeffectsofamn082onneuronalapoptosisinratsaftertraumaticbraininjury AT leeyaolin neuroprotectiveeffectsofamn082onneuronalapoptosisinratsaftertraumaticbraininjury AT chiochungching neuroprotectiveeffectsofamn082onneuronalapoptosisinratsaftertraumaticbraininjury AT wangchechuan neuroprotectiveeffectsofamn082onneuronalapoptosisinratsaftertraumaticbraininjury |