Cargando…
Synthesis and Characterization of a Full-Length Infectious cDNA Clone of Tomato Mottle Mosaic Virus
Tomato mottle mosaic virus (ToMMV) is a noteworthy virus which belongs to the Virgaviridae family and causes serious economic losses in tomato. Here, we isolated and cloned the full-length genome of a ToMMV Chinese isolate (ToMMV-LN) from a naturally infected tomato (Solanum lycopersicum L.). Sequen...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8229035/ https://www.ncbi.nlm.nih.gov/pubmed/34206030 http://dx.doi.org/10.3390/v13061050 |
_version_ | 1783712881970249728 |
---|---|
author | Tu, Liqin Wu, Shuhua Gao, Danna Liu, Yong Zhu, Yuelin Ji, Yinghua |
author_facet | Tu, Liqin Wu, Shuhua Gao, Danna Liu, Yong Zhu, Yuelin Ji, Yinghua |
author_sort | Tu, Liqin |
collection | PubMed |
description | Tomato mottle mosaic virus (ToMMV) is a noteworthy virus which belongs to the Virgaviridae family and causes serious economic losses in tomato. Here, we isolated and cloned the full-length genome of a ToMMV Chinese isolate (ToMMV-LN) from a naturally infected tomato (Solanum lycopersicum L.). Sequence analysis showed that ToMMV-LN contains 6399 nucleotides (nts) and is most closely related to a ToMMV Mexican isolate with a sequence identity of 99.48%. Next, an infectious cDNA clone of ToMMV was constructed by a homologous recombination approach. Both the model host N. benthamiana and the natural hosts tomato and pepper developed severe symptoms upon agroinfiltration with pToMMV, which had a strong infectivity. Electron micrographs indicated that a large number of rigid rod-shaped ToMMV virions were observed from the agroinfiltrated N. benthamiana leaves. Finally, our results also confirmed that tomato plants inoculated with pToMMV led to a high infection rate of 100% in 4–5 weeks post-infiltration (wpi), while pepper plants inoculated with pToMMV led to an infection rate of 40–47% in 4–5 wpi. This is the first report of the development of a full-length infectious cDNA clone of ToMMV. We believe that this infectious clone will enable further studies of ToMMV genes function, pathogenicity and virus–host interaction. |
format | Online Article Text |
id | pubmed-8229035 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-82290352021-06-26 Synthesis and Characterization of a Full-Length Infectious cDNA Clone of Tomato Mottle Mosaic Virus Tu, Liqin Wu, Shuhua Gao, Danna Liu, Yong Zhu, Yuelin Ji, Yinghua Viruses Article Tomato mottle mosaic virus (ToMMV) is a noteworthy virus which belongs to the Virgaviridae family and causes serious economic losses in tomato. Here, we isolated and cloned the full-length genome of a ToMMV Chinese isolate (ToMMV-LN) from a naturally infected tomato (Solanum lycopersicum L.). Sequence analysis showed that ToMMV-LN contains 6399 nucleotides (nts) and is most closely related to a ToMMV Mexican isolate with a sequence identity of 99.48%. Next, an infectious cDNA clone of ToMMV was constructed by a homologous recombination approach. Both the model host N. benthamiana and the natural hosts tomato and pepper developed severe symptoms upon agroinfiltration with pToMMV, which had a strong infectivity. Electron micrographs indicated that a large number of rigid rod-shaped ToMMV virions were observed from the agroinfiltrated N. benthamiana leaves. Finally, our results also confirmed that tomato plants inoculated with pToMMV led to a high infection rate of 100% in 4–5 weeks post-infiltration (wpi), while pepper plants inoculated with pToMMV led to an infection rate of 40–47% in 4–5 wpi. This is the first report of the development of a full-length infectious cDNA clone of ToMMV. We believe that this infectious clone will enable further studies of ToMMV genes function, pathogenicity and virus–host interaction. MDPI 2021-06-01 /pmc/articles/PMC8229035/ /pubmed/34206030 http://dx.doi.org/10.3390/v13061050 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Tu, Liqin Wu, Shuhua Gao, Danna Liu, Yong Zhu, Yuelin Ji, Yinghua Synthesis and Characterization of a Full-Length Infectious cDNA Clone of Tomato Mottle Mosaic Virus |
title | Synthesis and Characterization of a Full-Length Infectious cDNA Clone of Tomato Mottle Mosaic Virus |
title_full | Synthesis and Characterization of a Full-Length Infectious cDNA Clone of Tomato Mottle Mosaic Virus |
title_fullStr | Synthesis and Characterization of a Full-Length Infectious cDNA Clone of Tomato Mottle Mosaic Virus |
title_full_unstemmed | Synthesis and Characterization of a Full-Length Infectious cDNA Clone of Tomato Mottle Mosaic Virus |
title_short | Synthesis and Characterization of a Full-Length Infectious cDNA Clone of Tomato Mottle Mosaic Virus |
title_sort | synthesis and characterization of a full-length infectious cdna clone of tomato mottle mosaic virus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8229035/ https://www.ncbi.nlm.nih.gov/pubmed/34206030 http://dx.doi.org/10.3390/v13061050 |
work_keys_str_mv | AT tuliqin synthesisandcharacterizationofafulllengthinfectiouscdnacloneoftomatomottlemosaicvirus AT wushuhua synthesisandcharacterizationofafulllengthinfectiouscdnacloneoftomatomottlemosaicvirus AT gaodanna synthesisandcharacterizationofafulllengthinfectiouscdnacloneoftomatomottlemosaicvirus AT liuyong synthesisandcharacterizationofafulllengthinfectiouscdnacloneoftomatomottlemosaicvirus AT zhuyuelin synthesisandcharacterizationofafulllengthinfectiouscdnacloneoftomatomottlemosaicvirus AT jiyinghua synthesisandcharacterizationofafulllengthinfectiouscdnacloneoftomatomottlemosaicvirus |