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The Origin of the Cyathea delgadii Sternb. Somatic Embryos Is Determined by the Developmental State of Donor Tissue and Mutual Balance of Selected Metabolites

Somatic embryogenesis is the formation of a plant embryo from a cell other than the product of gametic fusion. The need to recognize the determinants of somatic cell fate has prompted investigations on how endogenous factors of donor tissues can determine the pattern of somatic embryo origin. The un...

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Autores principales: Mikuła, Anna, Tomaszewicz, Wojciech, Dziurka, Michał, Kaźmierczak, Andrzej, Grzyb, Małgorzata, Sobczak, Mirosław, Zdańkowski, Piotr, Rybczyński, Jan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8229038/
https://www.ncbi.nlm.nih.gov/pubmed/34199921
http://dx.doi.org/10.3390/cells10061388
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author Mikuła, Anna
Tomaszewicz, Wojciech
Dziurka, Michał
Kaźmierczak, Andrzej
Grzyb, Małgorzata
Sobczak, Mirosław
Zdańkowski, Piotr
Rybczyński, Jan
author_facet Mikuła, Anna
Tomaszewicz, Wojciech
Dziurka, Michał
Kaźmierczak, Andrzej
Grzyb, Małgorzata
Sobczak, Mirosław
Zdańkowski, Piotr
Rybczyński, Jan
author_sort Mikuła, Anna
collection PubMed
description Somatic embryogenesis is the formation of a plant embryo from a cell other than the product of gametic fusion. The need to recognize the determinants of somatic cell fate has prompted investigations on how endogenous factors of donor tissues can determine the pattern of somatic embryo origin. The undertaking of this study was enabled by the newly developed experimental system of somatic embryogenesis of the tree fern Cyathea delgadii Sternb., in which the embryos are produced in hormone-free medium. The contents of 89 endogenous compounds (such as sugars, auxins, cytokinins, gibberellins, stress-related hormones, phenolic acids, polyamines, and amino acids) and cytomorphological features were compared between two types of explants giving rise to somatic embryos of unicellular or multicellular origin. We found that a large content of maltose, 1-kestose, abscisic acid, biologically active gibberellins, and phenolic acids was characteristic for single-cell somatic embryo formation pattern. In contrast, high levels of starch, callose, kinetin riboside, arginine, and ethylene promoted their multicellular origin. Networks for visualization of the relations between studied compounds were constructed based on the data obtained from analyses of a Pearson correlation coefficient heatmap. Our findings present for the first time detailed features of donor tissue that can play an important role in the somatic-to-embryogenic transition and the somatic embryo origin.
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spelling pubmed-82290382021-06-26 The Origin of the Cyathea delgadii Sternb. Somatic Embryos Is Determined by the Developmental State of Donor Tissue and Mutual Balance of Selected Metabolites Mikuła, Anna Tomaszewicz, Wojciech Dziurka, Michał Kaźmierczak, Andrzej Grzyb, Małgorzata Sobczak, Mirosław Zdańkowski, Piotr Rybczyński, Jan Cells Article Somatic embryogenesis is the formation of a plant embryo from a cell other than the product of gametic fusion. The need to recognize the determinants of somatic cell fate has prompted investigations on how endogenous factors of donor tissues can determine the pattern of somatic embryo origin. The undertaking of this study was enabled by the newly developed experimental system of somatic embryogenesis of the tree fern Cyathea delgadii Sternb., in which the embryos are produced in hormone-free medium. The contents of 89 endogenous compounds (such as sugars, auxins, cytokinins, gibberellins, stress-related hormones, phenolic acids, polyamines, and amino acids) and cytomorphological features were compared between two types of explants giving rise to somatic embryos of unicellular or multicellular origin. We found that a large content of maltose, 1-kestose, abscisic acid, biologically active gibberellins, and phenolic acids was characteristic for single-cell somatic embryo formation pattern. In contrast, high levels of starch, callose, kinetin riboside, arginine, and ethylene promoted their multicellular origin. Networks for visualization of the relations between studied compounds were constructed based on the data obtained from analyses of a Pearson correlation coefficient heatmap. Our findings present for the first time detailed features of donor tissue that can play an important role in the somatic-to-embryogenic transition and the somatic embryo origin. MDPI 2021-06-04 /pmc/articles/PMC8229038/ /pubmed/34199921 http://dx.doi.org/10.3390/cells10061388 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mikuła, Anna
Tomaszewicz, Wojciech
Dziurka, Michał
Kaźmierczak, Andrzej
Grzyb, Małgorzata
Sobczak, Mirosław
Zdańkowski, Piotr
Rybczyński, Jan
The Origin of the Cyathea delgadii Sternb. Somatic Embryos Is Determined by the Developmental State of Donor Tissue and Mutual Balance of Selected Metabolites
title The Origin of the Cyathea delgadii Sternb. Somatic Embryos Is Determined by the Developmental State of Donor Tissue and Mutual Balance of Selected Metabolites
title_full The Origin of the Cyathea delgadii Sternb. Somatic Embryos Is Determined by the Developmental State of Donor Tissue and Mutual Balance of Selected Metabolites
title_fullStr The Origin of the Cyathea delgadii Sternb. Somatic Embryos Is Determined by the Developmental State of Donor Tissue and Mutual Balance of Selected Metabolites
title_full_unstemmed The Origin of the Cyathea delgadii Sternb. Somatic Embryos Is Determined by the Developmental State of Donor Tissue and Mutual Balance of Selected Metabolites
title_short The Origin of the Cyathea delgadii Sternb. Somatic Embryos Is Determined by the Developmental State of Donor Tissue and Mutual Balance of Selected Metabolites
title_sort origin of the cyathea delgadii sternb. somatic embryos is determined by the developmental state of donor tissue and mutual balance of selected metabolites
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8229038/
https://www.ncbi.nlm.nih.gov/pubmed/34199921
http://dx.doi.org/10.3390/cells10061388
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