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Mesenchymal Stem Cells as a Gene Delivery Tool: Promise, Problems, and Prospects

The cell-based approach in gene therapy arises as a promising strategy to provide safe, targeted, and efficient gene delivery. Owing to their unique features, as homing and tumor-tropism, mesenchymal stem cells (MSCs) have recently been introduced as an encouraging vehicle in gene therapy. Neverthel...

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Detalles Bibliográficos
Autores principales: Attia, Noha, Mashal, Mohamed, Puras, Gustavo, Pedraz, Jose Luis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8229096/
https://www.ncbi.nlm.nih.gov/pubmed/34200425
http://dx.doi.org/10.3390/pharmaceutics13060843
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author Attia, Noha
Mashal, Mohamed
Puras, Gustavo
Pedraz, Jose Luis
author_facet Attia, Noha
Mashal, Mohamed
Puras, Gustavo
Pedraz, Jose Luis
author_sort Attia, Noha
collection PubMed
description The cell-based approach in gene therapy arises as a promising strategy to provide safe, targeted, and efficient gene delivery. Owing to their unique features, as homing and tumor-tropism, mesenchymal stem cells (MSCs) have recently been introduced as an encouraging vehicle in gene therapy. Nevertheless, non-viral transfer of nucleic acids into MSCs remains limited due to various factors related to the main stakeholders of the process (e.g., nucleic acids, carriers, or cells). In this review, we have summarized the main types of nucleic acids used to transfect MSCs, the pros and cons, and applications of each. Then, we have emphasized on the most efficient lipid-based carriers for nucleic acids to MSCs, their main features, and some of their applications. While a myriad of studies have demonstrated the therapeutic potential for engineered MSCs therapy in various illnesses, optimization for clinical use is an ongoing challenge. On the way of improvement, genetically modified MSCs have been combined with various novel techniques and tools (e.g., exosomes, spheroids, 3D-Bioprinting, etc.,) aiming for more efficient and safe applications in biomedicine.
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spelling pubmed-82290962021-06-26 Mesenchymal Stem Cells as a Gene Delivery Tool: Promise, Problems, and Prospects Attia, Noha Mashal, Mohamed Puras, Gustavo Pedraz, Jose Luis Pharmaceutics Review The cell-based approach in gene therapy arises as a promising strategy to provide safe, targeted, and efficient gene delivery. Owing to their unique features, as homing and tumor-tropism, mesenchymal stem cells (MSCs) have recently been introduced as an encouraging vehicle in gene therapy. Nevertheless, non-viral transfer of nucleic acids into MSCs remains limited due to various factors related to the main stakeholders of the process (e.g., nucleic acids, carriers, or cells). In this review, we have summarized the main types of nucleic acids used to transfect MSCs, the pros and cons, and applications of each. Then, we have emphasized on the most efficient lipid-based carriers for nucleic acids to MSCs, their main features, and some of their applications. While a myriad of studies have demonstrated the therapeutic potential for engineered MSCs therapy in various illnesses, optimization for clinical use is an ongoing challenge. On the way of improvement, genetically modified MSCs have been combined with various novel techniques and tools (e.g., exosomes, spheroids, 3D-Bioprinting, etc.,) aiming for more efficient and safe applications in biomedicine. MDPI 2021-06-07 /pmc/articles/PMC8229096/ /pubmed/34200425 http://dx.doi.org/10.3390/pharmaceutics13060843 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Attia, Noha
Mashal, Mohamed
Puras, Gustavo
Pedraz, Jose Luis
Mesenchymal Stem Cells as a Gene Delivery Tool: Promise, Problems, and Prospects
title Mesenchymal Stem Cells as a Gene Delivery Tool: Promise, Problems, and Prospects
title_full Mesenchymal Stem Cells as a Gene Delivery Tool: Promise, Problems, and Prospects
title_fullStr Mesenchymal Stem Cells as a Gene Delivery Tool: Promise, Problems, and Prospects
title_full_unstemmed Mesenchymal Stem Cells as a Gene Delivery Tool: Promise, Problems, and Prospects
title_short Mesenchymal Stem Cells as a Gene Delivery Tool: Promise, Problems, and Prospects
title_sort mesenchymal stem cells as a gene delivery tool: promise, problems, and prospects
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8229096/
https://www.ncbi.nlm.nih.gov/pubmed/34200425
http://dx.doi.org/10.3390/pharmaceutics13060843
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