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Prevalence of Linda Virus Neutralizing Antibodies in the Austrian Pig Population

A novel pestivirus species, termed Lateral-shaking Inducing Neuro-Degenerative Agent virus (LindaV), was discovered in a piglet-producing farm in Austria in 2015 related to severe congenital tremor cases. Since the initial outbreak LindaV has not been found anywhere else. In this study, we determine...

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Autores principales: Kiesler, Alexandra, Plankensteiner, Jakob, Schwarz, Lukas, Riedel, Christiane, Seitz, Kerstin, Mötz, Marlene, Ladinig, Andrea, Lamp, Benjamin, Rümenapf, Till
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8229103/
https://www.ncbi.nlm.nih.gov/pubmed/34071946
http://dx.doi.org/10.3390/v13061001
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author Kiesler, Alexandra
Plankensteiner, Jakob
Schwarz, Lukas
Riedel, Christiane
Seitz, Kerstin
Mötz, Marlene
Ladinig, Andrea
Lamp, Benjamin
Rümenapf, Till
author_facet Kiesler, Alexandra
Plankensteiner, Jakob
Schwarz, Lukas
Riedel, Christiane
Seitz, Kerstin
Mötz, Marlene
Ladinig, Andrea
Lamp, Benjamin
Rümenapf, Till
author_sort Kiesler, Alexandra
collection PubMed
description A novel pestivirus species, termed Lateral-shaking Inducing Neuro-Degenerative Agent virus (LindaV), was discovered in a piglet-producing farm in Austria in 2015 related to severe congenital tremor cases. Since the initial outbreak LindaV has not been found anywhere else. In this study, we determined the seroprevalence of LindaV infections in the domestic pig population of Austria. A fluorophore labeled infectious cDNA clone of LindaV (mCherry-LindaV) was generated and used in serum virus neutralization (SVN) assays for the detection of LindaV specific neutralizing antibodies in porcine serum samples. In total, 637 sera from sows and gilts from five federal states of Austria, collected between the years 2015 and 2020, were analyzed. We identified a single serum showing a high neutralizing antibody titer, that originated from a farm (Farm S2) in the proximity of the initially affected farm. The analysis of 57 additional sera from Farm S2 revealed a wider spread of LindaV in this pig herd. Furthermore, a second LindaV strain originating from this farm could be isolated in cell culture and was further characterized at the genetic level. Possible transmission routes and virus reservoir hosts of this emerging porcine virus need to be addressed in future studies.
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spelling pubmed-82291032021-06-26 Prevalence of Linda Virus Neutralizing Antibodies in the Austrian Pig Population Kiesler, Alexandra Plankensteiner, Jakob Schwarz, Lukas Riedel, Christiane Seitz, Kerstin Mötz, Marlene Ladinig, Andrea Lamp, Benjamin Rümenapf, Till Viruses Article A novel pestivirus species, termed Lateral-shaking Inducing Neuro-Degenerative Agent virus (LindaV), was discovered in a piglet-producing farm in Austria in 2015 related to severe congenital tremor cases. Since the initial outbreak LindaV has not been found anywhere else. In this study, we determined the seroprevalence of LindaV infections in the domestic pig population of Austria. A fluorophore labeled infectious cDNA clone of LindaV (mCherry-LindaV) was generated and used in serum virus neutralization (SVN) assays for the detection of LindaV specific neutralizing antibodies in porcine serum samples. In total, 637 sera from sows and gilts from five federal states of Austria, collected between the years 2015 and 2020, were analyzed. We identified a single serum showing a high neutralizing antibody titer, that originated from a farm (Farm S2) in the proximity of the initially affected farm. The analysis of 57 additional sera from Farm S2 revealed a wider spread of LindaV in this pig herd. Furthermore, a second LindaV strain originating from this farm could be isolated in cell culture and was further characterized at the genetic level. Possible transmission routes and virus reservoir hosts of this emerging porcine virus need to be addressed in future studies. MDPI 2021-05-27 /pmc/articles/PMC8229103/ /pubmed/34071946 http://dx.doi.org/10.3390/v13061001 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kiesler, Alexandra
Plankensteiner, Jakob
Schwarz, Lukas
Riedel, Christiane
Seitz, Kerstin
Mötz, Marlene
Ladinig, Andrea
Lamp, Benjamin
Rümenapf, Till
Prevalence of Linda Virus Neutralizing Antibodies in the Austrian Pig Population
title Prevalence of Linda Virus Neutralizing Antibodies in the Austrian Pig Population
title_full Prevalence of Linda Virus Neutralizing Antibodies in the Austrian Pig Population
title_fullStr Prevalence of Linda Virus Neutralizing Antibodies in the Austrian Pig Population
title_full_unstemmed Prevalence of Linda Virus Neutralizing Antibodies in the Austrian Pig Population
title_short Prevalence of Linda Virus Neutralizing Antibodies in the Austrian Pig Population
title_sort prevalence of linda virus neutralizing antibodies in the austrian pig population
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8229103/
https://www.ncbi.nlm.nih.gov/pubmed/34071946
http://dx.doi.org/10.3390/v13061001
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