First insights into molecular basis identification of 16 s ribosomal RNA gene of Staphylococcus aureus isolated from Sudan

OBJECTIVE: In this study, we analyzed the molecular evolution of Staphylococcus aureus isolates using 16S rRNA gene and phylogenetic analysis to detect the prevalence of S. aureus infections in Sudan. RESULTS: Molecular detection of S. aureus has shown that 20 (43.47%) of patients were positive for...

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Autores principales: Gumaa, Manal A., Idris, Abeer Babiker, Bilal, N. E., Hassan, Mohamed A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8229275/
https://www.ncbi.nlm.nih.gov/pubmed/34172074
http://dx.doi.org/10.1186/s13104-021-05569-w
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author Gumaa, Manal A.
Idris, Abeer Babiker
Bilal, N. E.
Hassan, Mohamed A.
author_facet Gumaa, Manal A.
Idris, Abeer Babiker
Bilal, N. E.
Hassan, Mohamed A.
author_sort Gumaa, Manal A.
collection PubMed
description OBJECTIVE: In this study, we analyzed the molecular evolution of Staphylococcus aureus isolates using 16S rRNA gene and phylogenetic analysis to detect the prevalence of S. aureus infections in Sudan. RESULTS: Molecular detection of S. aureus has shown that 20 (43.47%) of patients were positive for S. aureus. The phylogenetic tree of 16S rRNA sequences was divided into three lineages of S. aureus isolates detected from wound infections in Sudan. Nucleotides base-pair substitution was appeared at position 249. This mutation do not linked with Macrolides, Lincosamides and Streptogramines b resistant phenotype. Further studies should investigate the effect of that mutation on resistance to other antibiotics. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13104-021-05569-w.
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spelling pubmed-82292752021-06-28 First insights into molecular basis identification of 16 s ribosomal RNA gene of Staphylococcus aureus isolated from Sudan Gumaa, Manal A. Idris, Abeer Babiker Bilal, N. E. Hassan, Mohamed A. BMC Res Notes Research Note OBJECTIVE: In this study, we analyzed the molecular evolution of Staphylococcus aureus isolates using 16S rRNA gene and phylogenetic analysis to detect the prevalence of S. aureus infections in Sudan. RESULTS: Molecular detection of S. aureus has shown that 20 (43.47%) of patients were positive for S. aureus. The phylogenetic tree of 16S rRNA sequences was divided into three lineages of S. aureus isolates detected from wound infections in Sudan. Nucleotides base-pair substitution was appeared at position 249. This mutation do not linked with Macrolides, Lincosamides and Streptogramines b resistant phenotype. Further studies should investigate the effect of that mutation on resistance to other antibiotics. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13104-021-05569-w. BioMed Central 2021-06-25 /pmc/articles/PMC8229275/ /pubmed/34172074 http://dx.doi.org/10.1186/s13104-021-05569-w Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Note
Gumaa, Manal A.
Idris, Abeer Babiker
Bilal, N. E.
Hassan, Mohamed A.
First insights into molecular basis identification of 16 s ribosomal RNA gene of Staphylococcus aureus isolated from Sudan
title First insights into molecular basis identification of 16 s ribosomal RNA gene of Staphylococcus aureus isolated from Sudan
title_full First insights into molecular basis identification of 16 s ribosomal RNA gene of Staphylococcus aureus isolated from Sudan
title_fullStr First insights into molecular basis identification of 16 s ribosomal RNA gene of Staphylococcus aureus isolated from Sudan
title_full_unstemmed First insights into molecular basis identification of 16 s ribosomal RNA gene of Staphylococcus aureus isolated from Sudan
title_short First insights into molecular basis identification of 16 s ribosomal RNA gene of Staphylococcus aureus isolated from Sudan
title_sort first insights into molecular basis identification of 16 s ribosomal rna gene of staphylococcus aureus isolated from sudan
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8229275/
https://www.ncbi.nlm.nih.gov/pubmed/34172074
http://dx.doi.org/10.1186/s13104-021-05569-w
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