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The Anti-Oxidative and Anti-Neuroinflammatory Effects of Sargassum horneri by Heme Oxygenase-1 Induction in BV2 and HT22 Cells
Sargassum horneri is used as a traditional medicinal agent and exhibits various pharmacological effects. In this study, we found that the 70% EtOH extract contained 34.37 ± 0.75 μg/mg fucosterol. We tested the antioxidant activities of the 70% EtOH extracts and their fractions. The CH(2)Cl(2)-solubl...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8229279/ https://www.ncbi.nlm.nih.gov/pubmed/34071911 http://dx.doi.org/10.3390/antiox10060859 |
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author | Ko, Wonmin Lee, Hwan Kim, Nayeon Jo, Hee Geun Woo, Eun-Rhan Lee, Kyounghoon Han, Young Seok Park, Sang Rul Ahn, Ginnae Cheong, Sun Hee Lee, Dong-Sung |
author_facet | Ko, Wonmin Lee, Hwan Kim, Nayeon Jo, Hee Geun Woo, Eun-Rhan Lee, Kyounghoon Han, Young Seok Park, Sang Rul Ahn, Ginnae Cheong, Sun Hee Lee, Dong-Sung |
author_sort | Ko, Wonmin |
collection | PubMed |
description | Sargassum horneri is used as a traditional medicinal agent and exhibits various pharmacological effects. In this study, we found that the 70% EtOH extract contained 34.37 ± 0.75 μg/mg fucosterol. We tested the antioxidant activities of the 70% EtOH extracts and their fractions. The CH(2)Cl(2)-soluble fraction showed the strongest DPPH and ABTS radical scavenging activities. Next, we evaluated the anti-neuroinflammatory effects of S. horneri on lipopolysaccharide (LPS)-stimulated BV2 cells. Pretreatment with the extract and fractions suppressed LPS-induced production of nitric oxide (NO) in BV2 cells. The 70% EtOH, CH(2)Cl(2)-soluble fraction, and water-soluble fraction inhibited the production of prostaglandin E2, interleukin-6, and tumor necrosis factor-α, as well as markedly blocking LPS-induced expression of inducible NO synthase and cyclooxygenase-2 via inactivation of the nuclear factor-kappa B pathway. In addition, the CH(2)Cl(2)-soluble fraction showed the most remarkable heme oxygenase (HO)-1 expression effects and increased nuclear erythroid 2-related factor translocation in the nucleus. In HT22 cells, the CH(2)Cl(2)-soluble fraction inhibited cell damage and ROS production caused by glutamate via the regulation of HO-1. Therefore, CH(2)Cl(2)-soluble fractions of S. horneri can attenuate oxidative action and neuroinflammatory responses via HO-1 induction, demonstrating their potential in the treatment of neuroinflammatory diseases. |
format | Online Article Text |
id | pubmed-8229279 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-82292792021-06-26 The Anti-Oxidative and Anti-Neuroinflammatory Effects of Sargassum horneri by Heme Oxygenase-1 Induction in BV2 and HT22 Cells Ko, Wonmin Lee, Hwan Kim, Nayeon Jo, Hee Geun Woo, Eun-Rhan Lee, Kyounghoon Han, Young Seok Park, Sang Rul Ahn, Ginnae Cheong, Sun Hee Lee, Dong-Sung Antioxidants (Basel) Article Sargassum horneri is used as a traditional medicinal agent and exhibits various pharmacological effects. In this study, we found that the 70% EtOH extract contained 34.37 ± 0.75 μg/mg fucosterol. We tested the antioxidant activities of the 70% EtOH extracts and their fractions. The CH(2)Cl(2)-soluble fraction showed the strongest DPPH and ABTS radical scavenging activities. Next, we evaluated the anti-neuroinflammatory effects of S. horneri on lipopolysaccharide (LPS)-stimulated BV2 cells. Pretreatment with the extract and fractions suppressed LPS-induced production of nitric oxide (NO) in BV2 cells. The 70% EtOH, CH(2)Cl(2)-soluble fraction, and water-soluble fraction inhibited the production of prostaglandin E2, interleukin-6, and tumor necrosis factor-α, as well as markedly blocking LPS-induced expression of inducible NO synthase and cyclooxygenase-2 via inactivation of the nuclear factor-kappa B pathway. In addition, the CH(2)Cl(2)-soluble fraction showed the most remarkable heme oxygenase (HO)-1 expression effects and increased nuclear erythroid 2-related factor translocation in the nucleus. In HT22 cells, the CH(2)Cl(2)-soluble fraction inhibited cell damage and ROS production caused by glutamate via the regulation of HO-1. Therefore, CH(2)Cl(2)-soluble fractions of S. horneri can attenuate oxidative action and neuroinflammatory responses via HO-1 induction, demonstrating their potential in the treatment of neuroinflammatory diseases. MDPI 2021-05-27 /pmc/articles/PMC8229279/ /pubmed/34071911 http://dx.doi.org/10.3390/antiox10060859 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ko, Wonmin Lee, Hwan Kim, Nayeon Jo, Hee Geun Woo, Eun-Rhan Lee, Kyounghoon Han, Young Seok Park, Sang Rul Ahn, Ginnae Cheong, Sun Hee Lee, Dong-Sung The Anti-Oxidative and Anti-Neuroinflammatory Effects of Sargassum horneri by Heme Oxygenase-1 Induction in BV2 and HT22 Cells |
title | The Anti-Oxidative and Anti-Neuroinflammatory Effects of Sargassum horneri by Heme Oxygenase-1 Induction in BV2 and HT22 Cells |
title_full | The Anti-Oxidative and Anti-Neuroinflammatory Effects of Sargassum horneri by Heme Oxygenase-1 Induction in BV2 and HT22 Cells |
title_fullStr | The Anti-Oxidative and Anti-Neuroinflammatory Effects of Sargassum horneri by Heme Oxygenase-1 Induction in BV2 and HT22 Cells |
title_full_unstemmed | The Anti-Oxidative and Anti-Neuroinflammatory Effects of Sargassum horneri by Heme Oxygenase-1 Induction in BV2 and HT22 Cells |
title_short | The Anti-Oxidative and Anti-Neuroinflammatory Effects of Sargassum horneri by Heme Oxygenase-1 Induction in BV2 and HT22 Cells |
title_sort | anti-oxidative and anti-neuroinflammatory effects of sargassum horneri by heme oxygenase-1 induction in bv2 and ht22 cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8229279/ https://www.ncbi.nlm.nih.gov/pubmed/34071911 http://dx.doi.org/10.3390/antiox10060859 |
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