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Experimental Platform to Study Spiking Pattern Propagation in Modular Networks In Vitro

The structured organization of connectivity in neural networks is associated with highly efficient information propagation and processing in the brain, in contrast with disordered homogeneous network architectures. Using microfluidic methods, we engineered modular networks of cultures using dissocia...

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Detalles Bibliográficos
Autores principales: Pigareva, Yana, Gladkov, Arseniy, Kolpakov, Vladimir, Mukhina, Irina, Bukatin, Anton, Kazantsev, Victor B., Pimashkin, Alexey
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8229331/
https://www.ncbi.nlm.nih.gov/pubmed/34071257
http://dx.doi.org/10.3390/brainsci11060717
Descripción
Sumario:The structured organization of connectivity in neural networks is associated with highly efficient information propagation and processing in the brain, in contrast with disordered homogeneous network architectures. Using microfluidic methods, we engineered modular networks of cultures using dissociated cells with unidirectional synaptic connections formed by asymmetric microchannels. The complexity of the microchannel geometry defined the strength of the synaptic connectivity and the properties of spiking activity propagation. In this study, we developed an experimental platform to study the effects of synaptic plasticity on a network level with predefined locations of unidirectionally connected cellular assemblies using multisite extracellular electrophysiology.