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Use of a Specific Phage Cocktail for Soft Rot Control on Ware Potatoes: A Case Study

Using bacteriophages (bacterial viruses) to control pathogenic bacteria is a promising approach in horticulture. However, the application of this strategy in real conditions requires compliance with particular technological and environmental restraints. The presented paper concerns the process of ph...

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Autores principales: Bugaeva, Eugenia N., Voronina, Maya V., Vasiliev, Dmitry M., Lukianova, Anna A., Landyshev, Nikolay N., Ignatov, Alexander N., Miroshnikov, Konstantin A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8229397/
https://www.ncbi.nlm.nih.gov/pubmed/34201375
http://dx.doi.org/10.3390/v13061095
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author Bugaeva, Eugenia N.
Voronina, Maya V.
Vasiliev, Dmitry M.
Lukianova, Anna A.
Landyshev, Nikolay N.
Ignatov, Alexander N.
Miroshnikov, Konstantin A.
author_facet Bugaeva, Eugenia N.
Voronina, Maya V.
Vasiliev, Dmitry M.
Lukianova, Anna A.
Landyshev, Nikolay N.
Ignatov, Alexander N.
Miroshnikov, Konstantin A.
author_sort Bugaeva, Eugenia N.
collection PubMed
description Using bacteriophages (bacterial viruses) to control pathogenic bacteria is a promising approach in horticulture. However, the application of this strategy in real conditions requires compliance with particular technological and environmental restraints. The presented paper concerns the process of phage selection to create a cocktail that is efficient against the circulating causal agents of potato soft rot. The resulting phage cocktail causes a complete lysis of a mixture of circulating pectobacterial strains in vitro. In the context of being used to treat ware potatoes during off-season storage, the protocol of phage application via the humidity maintenance system was designed. The phage cocktail was shown to reduce the population of Pectobacterium spp. 10–12-fold, achieving a population that was below a symptomatic threshold.
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spelling pubmed-82293972021-06-26 Use of a Specific Phage Cocktail for Soft Rot Control on Ware Potatoes: A Case Study Bugaeva, Eugenia N. Voronina, Maya V. Vasiliev, Dmitry M. Lukianova, Anna A. Landyshev, Nikolay N. Ignatov, Alexander N. Miroshnikov, Konstantin A. Viruses Communication Using bacteriophages (bacterial viruses) to control pathogenic bacteria is a promising approach in horticulture. However, the application of this strategy in real conditions requires compliance with particular technological and environmental restraints. The presented paper concerns the process of phage selection to create a cocktail that is efficient against the circulating causal agents of potato soft rot. The resulting phage cocktail causes a complete lysis of a mixture of circulating pectobacterial strains in vitro. In the context of being used to treat ware potatoes during off-season storage, the protocol of phage application via the humidity maintenance system was designed. The phage cocktail was shown to reduce the population of Pectobacterium spp. 10–12-fold, achieving a population that was below a symptomatic threshold. MDPI 2021-06-08 /pmc/articles/PMC8229397/ /pubmed/34201375 http://dx.doi.org/10.3390/v13061095 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Bugaeva, Eugenia N.
Voronina, Maya V.
Vasiliev, Dmitry M.
Lukianova, Anna A.
Landyshev, Nikolay N.
Ignatov, Alexander N.
Miroshnikov, Konstantin A.
Use of a Specific Phage Cocktail for Soft Rot Control on Ware Potatoes: A Case Study
title Use of a Specific Phage Cocktail for Soft Rot Control on Ware Potatoes: A Case Study
title_full Use of a Specific Phage Cocktail for Soft Rot Control on Ware Potatoes: A Case Study
title_fullStr Use of a Specific Phage Cocktail for Soft Rot Control on Ware Potatoes: A Case Study
title_full_unstemmed Use of a Specific Phage Cocktail for Soft Rot Control on Ware Potatoes: A Case Study
title_short Use of a Specific Phage Cocktail for Soft Rot Control on Ware Potatoes: A Case Study
title_sort use of a specific phage cocktail for soft rot control on ware potatoes: a case study
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8229397/
https://www.ncbi.nlm.nih.gov/pubmed/34201375
http://dx.doi.org/10.3390/v13061095
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