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Genome Mining for Antimicrobial Compounds in Wild Marine Animals-Associated Enterococci
New ecosystems are being actively mined for new bioactive compounds. Because of the large amount of unexplored biodiversity, bacteria from marine environments are especially promising. Further, host-associated microbes are of special interest because of their low toxicity and compatibility with host...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8229437/ https://www.ncbi.nlm.nih.gov/pubmed/34204046 http://dx.doi.org/10.3390/md19060328 |
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author | Prichula, Janira Primon-Barros, Muriel Luz, Romeu C. Z. Castro, Ícaro M. S. Paim, Thiago G. S. Tavares, Maurício Ligabue-Braun, Rodrigo d’Azevedo, Pedro A. Frazzon, Jeverson Frazzon, Ana P. G. Seixas, Adriana Gilmore, Michael S. |
author_facet | Prichula, Janira Primon-Barros, Muriel Luz, Romeu C. Z. Castro, Ícaro M. S. Paim, Thiago G. S. Tavares, Maurício Ligabue-Braun, Rodrigo d’Azevedo, Pedro A. Frazzon, Jeverson Frazzon, Ana P. G. Seixas, Adriana Gilmore, Michael S. |
author_sort | Prichula, Janira |
collection | PubMed |
description | New ecosystems are being actively mined for new bioactive compounds. Because of the large amount of unexplored biodiversity, bacteria from marine environments are especially promising. Further, host-associated microbes are of special interest because of their low toxicity and compatibility with host health. Here, we identified and characterized biosynthetic gene clusters encoding antimicrobial compounds in host-associated enterococci recovered from fecal samples of wild marine animals remote from human-affected ecosystems. Putative biosynthetic gene clusters in the genomes of 22 Enterococcus strains of marine origin were predicted using antiSMASH5 and Bagel4 bioinformatic software. At least one gene cluster encoding a putative bioactive compound precursor was identified in each genome. Collectively, 73 putative antimicrobial compounds were identified, including 61 bacteriocins (83.56%), 10 terpenes (13.70%), and 2 (2.74%) related to putative nonribosomal peptides (NRPs). Two of the species studied, Enterococcus avium and Enterococcus mundtti, are rare causes of human disease and were found to lack any known pathogenic determinants but yet possessed bacteriocin biosynthetic genes, suggesting possible additional utility as probiotics. Wild marine animal-associated enterococci from human-remote ecosystems provide a potentially rich source for new antimicrobial compounds of therapeutic and industrial value and potential probiotic application. |
format | Online Article Text |
id | pubmed-8229437 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-82294372021-06-26 Genome Mining for Antimicrobial Compounds in Wild Marine Animals-Associated Enterococci Prichula, Janira Primon-Barros, Muriel Luz, Romeu C. Z. Castro, Ícaro M. S. Paim, Thiago G. S. Tavares, Maurício Ligabue-Braun, Rodrigo d’Azevedo, Pedro A. Frazzon, Jeverson Frazzon, Ana P. G. Seixas, Adriana Gilmore, Michael S. Mar Drugs Article New ecosystems are being actively mined for new bioactive compounds. Because of the large amount of unexplored biodiversity, bacteria from marine environments are especially promising. Further, host-associated microbes are of special interest because of their low toxicity and compatibility with host health. Here, we identified and characterized biosynthetic gene clusters encoding antimicrobial compounds in host-associated enterococci recovered from fecal samples of wild marine animals remote from human-affected ecosystems. Putative biosynthetic gene clusters in the genomes of 22 Enterococcus strains of marine origin were predicted using antiSMASH5 and Bagel4 bioinformatic software. At least one gene cluster encoding a putative bioactive compound precursor was identified in each genome. Collectively, 73 putative antimicrobial compounds were identified, including 61 bacteriocins (83.56%), 10 terpenes (13.70%), and 2 (2.74%) related to putative nonribosomal peptides (NRPs). Two of the species studied, Enterococcus avium and Enterococcus mundtti, are rare causes of human disease and were found to lack any known pathogenic determinants but yet possessed bacteriocin biosynthetic genes, suggesting possible additional utility as probiotics. Wild marine animal-associated enterococci from human-remote ecosystems provide a potentially rich source for new antimicrobial compounds of therapeutic and industrial value and potential probiotic application. MDPI 2021-06-06 /pmc/articles/PMC8229437/ /pubmed/34204046 http://dx.doi.org/10.3390/md19060328 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Prichula, Janira Primon-Barros, Muriel Luz, Romeu C. Z. Castro, Ícaro M. S. Paim, Thiago G. S. Tavares, Maurício Ligabue-Braun, Rodrigo d’Azevedo, Pedro A. Frazzon, Jeverson Frazzon, Ana P. G. Seixas, Adriana Gilmore, Michael S. Genome Mining for Antimicrobial Compounds in Wild Marine Animals-Associated Enterococci |
title | Genome Mining for Antimicrobial Compounds in Wild Marine Animals-Associated Enterococci |
title_full | Genome Mining for Antimicrobial Compounds in Wild Marine Animals-Associated Enterococci |
title_fullStr | Genome Mining for Antimicrobial Compounds in Wild Marine Animals-Associated Enterococci |
title_full_unstemmed | Genome Mining for Antimicrobial Compounds in Wild Marine Animals-Associated Enterococci |
title_short | Genome Mining for Antimicrobial Compounds in Wild Marine Animals-Associated Enterococci |
title_sort | genome mining for antimicrobial compounds in wild marine animals-associated enterococci |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8229437/ https://www.ncbi.nlm.nih.gov/pubmed/34204046 http://dx.doi.org/10.3390/md19060328 |
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