Cargando…
Comprehensive Metabolomic Analysis Reveals Dynamic Metabolic Reprogramming in Hep3B Cells with Aflatoxin B1 Exposure
Hepatitis B virus (HBV) infection and aflatoxin B1 (AFB1) exposure have been recognized as independent risk factors for the occurrence and development of hepatocellular carcinoma (HCC), but their combined impacts and the potential metabolic mechanisms remain poorly characterized. Here, a comprehensi...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8229485/ https://www.ncbi.nlm.nih.gov/pubmed/34072178 http://dx.doi.org/10.3390/toxins13060384 |
_version_ | 1783712987520958464 |
---|---|
author | Wang, Shufeng Yang, Xin Liu, Feng Wang, Xinzheng Zhang, Xuemin He, Kun Wang, Hongxia |
author_facet | Wang, Shufeng Yang, Xin Liu, Feng Wang, Xinzheng Zhang, Xuemin He, Kun Wang, Hongxia |
author_sort | Wang, Shufeng |
collection | PubMed |
description | Hepatitis B virus (HBV) infection and aflatoxin B1 (AFB1) exposure have been recognized as independent risk factors for the occurrence and development of hepatocellular carcinoma (HCC), but their combined impacts and the potential metabolic mechanisms remain poorly characterized. Here, a comprehensive non-targeted metabolomic study was performed following AFB1 exposed to Hep3B cells at two different doses: 16 μM and 32 μM. The metabolites were identified and quantified by an ultra-performance liquid chromatography-mass spectrometry (UPLC-MS)-based strategy. A total of 2679 metabolites were identified, and 392 differential metabolites were quantified among three groups. Pathway analysis indicated that dynamic metabolic reprogramming was induced by AFB1 and various pathways changed significantly, including purine and pyrimidine metabolism, hexosamine pathway and sialylation, fatty acid synthesis and oxidation, glycerophospholipid metabolism, tricarboxylic acid (TCA) cycle, glycolysis, and amino acid metabolism. To the best of our knowledge, the alteration of purine and pyrimidine metabolism and decrease of hexosamine pathways and sialylation with AFB1 exposure have not been reported. The results indicated that our metabolomic strategy is powerful to investigate the metabolome change of any stimulates due to its high sensitivity, high resolution, rapid separation, and good metabolome coverage. Besides, these findings provide an overview of the metabolic mechanisms of the AFB1 combined with HBV and new insight into the toxicological mechanism of AFB1. Thus, targeting these metabolic pathways may be an approach to prevent carcinogen-induced cancer, and these findings may provide potential drug targets for therapeutic intervention. |
format | Online Article Text |
id | pubmed-8229485 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-82294852021-06-26 Comprehensive Metabolomic Analysis Reveals Dynamic Metabolic Reprogramming in Hep3B Cells with Aflatoxin B1 Exposure Wang, Shufeng Yang, Xin Liu, Feng Wang, Xinzheng Zhang, Xuemin He, Kun Wang, Hongxia Toxins (Basel) Article Hepatitis B virus (HBV) infection and aflatoxin B1 (AFB1) exposure have been recognized as independent risk factors for the occurrence and development of hepatocellular carcinoma (HCC), but their combined impacts and the potential metabolic mechanisms remain poorly characterized. Here, a comprehensive non-targeted metabolomic study was performed following AFB1 exposed to Hep3B cells at two different doses: 16 μM and 32 μM. The metabolites were identified and quantified by an ultra-performance liquid chromatography-mass spectrometry (UPLC-MS)-based strategy. A total of 2679 metabolites were identified, and 392 differential metabolites were quantified among three groups. Pathway analysis indicated that dynamic metabolic reprogramming was induced by AFB1 and various pathways changed significantly, including purine and pyrimidine metabolism, hexosamine pathway and sialylation, fatty acid synthesis and oxidation, glycerophospholipid metabolism, tricarboxylic acid (TCA) cycle, glycolysis, and amino acid metabolism. To the best of our knowledge, the alteration of purine and pyrimidine metabolism and decrease of hexosamine pathways and sialylation with AFB1 exposure have not been reported. The results indicated that our metabolomic strategy is powerful to investigate the metabolome change of any stimulates due to its high sensitivity, high resolution, rapid separation, and good metabolome coverage. Besides, these findings provide an overview of the metabolic mechanisms of the AFB1 combined with HBV and new insight into the toxicological mechanism of AFB1. Thus, targeting these metabolic pathways may be an approach to prevent carcinogen-induced cancer, and these findings may provide potential drug targets for therapeutic intervention. MDPI 2021-05-27 /pmc/articles/PMC8229485/ /pubmed/34072178 http://dx.doi.org/10.3390/toxins13060384 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wang, Shufeng Yang, Xin Liu, Feng Wang, Xinzheng Zhang, Xuemin He, Kun Wang, Hongxia Comprehensive Metabolomic Analysis Reveals Dynamic Metabolic Reprogramming in Hep3B Cells with Aflatoxin B1 Exposure |
title | Comprehensive Metabolomic Analysis Reveals Dynamic Metabolic Reprogramming in Hep3B Cells with Aflatoxin B1 Exposure |
title_full | Comprehensive Metabolomic Analysis Reveals Dynamic Metabolic Reprogramming in Hep3B Cells with Aflatoxin B1 Exposure |
title_fullStr | Comprehensive Metabolomic Analysis Reveals Dynamic Metabolic Reprogramming in Hep3B Cells with Aflatoxin B1 Exposure |
title_full_unstemmed | Comprehensive Metabolomic Analysis Reveals Dynamic Metabolic Reprogramming in Hep3B Cells with Aflatoxin B1 Exposure |
title_short | Comprehensive Metabolomic Analysis Reveals Dynamic Metabolic Reprogramming in Hep3B Cells with Aflatoxin B1 Exposure |
title_sort | comprehensive metabolomic analysis reveals dynamic metabolic reprogramming in hep3b cells with aflatoxin b1 exposure |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8229485/ https://www.ncbi.nlm.nih.gov/pubmed/34072178 http://dx.doi.org/10.3390/toxins13060384 |
work_keys_str_mv | AT wangshufeng comprehensivemetabolomicanalysisrevealsdynamicmetabolicreprogramminginhep3bcellswithaflatoxinb1exposure AT yangxin comprehensivemetabolomicanalysisrevealsdynamicmetabolicreprogramminginhep3bcellswithaflatoxinb1exposure AT liufeng comprehensivemetabolomicanalysisrevealsdynamicmetabolicreprogramminginhep3bcellswithaflatoxinb1exposure AT wangxinzheng comprehensivemetabolomicanalysisrevealsdynamicmetabolicreprogramminginhep3bcellswithaflatoxinb1exposure AT zhangxuemin comprehensivemetabolomicanalysisrevealsdynamicmetabolicreprogramminginhep3bcellswithaflatoxinb1exposure AT hekun comprehensivemetabolomicanalysisrevealsdynamicmetabolicreprogramminginhep3bcellswithaflatoxinb1exposure AT wanghongxia comprehensivemetabolomicanalysisrevealsdynamicmetabolicreprogramminginhep3bcellswithaflatoxinb1exposure |