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Interplay between OmpA and RpoN Regulates Flagellar Synthesis in Stenotrophomonas maltophilia

OmpA, which encodes outer membrane protein A (OmpA), is the most abundant transcript in Stenotrophomonas maltophilia based on transcriptome analyses. The functions of OmpA, including adhesion, biofilm formation, drug resistance, and immune response targets, have been reported in some microorganisms,...

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Autores principales: Liao, Chun-Hsing, Chang, Chia-Lun, Huang, Hsin-Hui, Lin, Yi-Tsung, Li, Li-Hua, Yang, Tsuey-Ching
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8229486/
https://www.ncbi.nlm.nih.gov/pubmed/34199787
http://dx.doi.org/10.3390/microorganisms9061216
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author Liao, Chun-Hsing
Chang, Chia-Lun
Huang, Hsin-Hui
Lin, Yi-Tsung
Li, Li-Hua
Yang, Tsuey-Ching
author_facet Liao, Chun-Hsing
Chang, Chia-Lun
Huang, Hsin-Hui
Lin, Yi-Tsung
Li, Li-Hua
Yang, Tsuey-Ching
author_sort Liao, Chun-Hsing
collection PubMed
description OmpA, which encodes outer membrane protein A (OmpA), is the most abundant transcript in Stenotrophomonas maltophilia based on transcriptome analyses. The functions of OmpA, including adhesion, biofilm formation, drug resistance, and immune response targets, have been reported in some microorganisms, but few functions are known in S. maltophilia. This study aimed to elucidate the relationship between OmpA and swimming motility in S. maltophilia. KJΔOmpA, an ompA mutant, displayed compromised swimming and failure of conjugation-mediated plasmid transportation. The hierarchical organization of flagella synthesis genes in S. maltophilia was established by referencing the Pseudomonas aeruginosa model and was confirmed using mutant construction, qRT-PCR, and functional assays. Distinct from the P. aeruginosa model, rpoN, rather than fleQ and fliA, was at the top of the flagellar regulatory cascade in S. maltophilia. To elucidate the underlying mechanism responsible for ΔompA-mediated swimming compromise, transcriptome analysis of KJ and KJΔOmpA was performed and revealed rpoN downregulation in KJΔOmpA as the key element. The involvement of rpoN in ΔompA-mediated swimming compromise was verified using rpoN complementation, qRT-PCR, and function assays. Collectively, OmpA, which contributes to bacterial conjugation and swimming, is a promising target for adjuvant design in S. maltophilia.
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spelling pubmed-82294862021-06-26 Interplay between OmpA and RpoN Regulates Flagellar Synthesis in Stenotrophomonas maltophilia Liao, Chun-Hsing Chang, Chia-Lun Huang, Hsin-Hui Lin, Yi-Tsung Li, Li-Hua Yang, Tsuey-Ching Microorganisms Article OmpA, which encodes outer membrane protein A (OmpA), is the most abundant transcript in Stenotrophomonas maltophilia based on transcriptome analyses. The functions of OmpA, including adhesion, biofilm formation, drug resistance, and immune response targets, have been reported in some microorganisms, but few functions are known in S. maltophilia. This study aimed to elucidate the relationship between OmpA and swimming motility in S. maltophilia. KJΔOmpA, an ompA mutant, displayed compromised swimming and failure of conjugation-mediated plasmid transportation. The hierarchical organization of flagella synthesis genes in S. maltophilia was established by referencing the Pseudomonas aeruginosa model and was confirmed using mutant construction, qRT-PCR, and functional assays. Distinct from the P. aeruginosa model, rpoN, rather than fleQ and fliA, was at the top of the flagellar regulatory cascade in S. maltophilia. To elucidate the underlying mechanism responsible for ΔompA-mediated swimming compromise, transcriptome analysis of KJ and KJΔOmpA was performed and revealed rpoN downregulation in KJΔOmpA as the key element. The involvement of rpoN in ΔompA-mediated swimming compromise was verified using rpoN complementation, qRT-PCR, and function assays. Collectively, OmpA, which contributes to bacterial conjugation and swimming, is a promising target for adjuvant design in S. maltophilia. MDPI 2021-06-04 /pmc/articles/PMC8229486/ /pubmed/34199787 http://dx.doi.org/10.3390/microorganisms9061216 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liao, Chun-Hsing
Chang, Chia-Lun
Huang, Hsin-Hui
Lin, Yi-Tsung
Li, Li-Hua
Yang, Tsuey-Ching
Interplay between OmpA and RpoN Regulates Flagellar Synthesis in Stenotrophomonas maltophilia
title Interplay between OmpA and RpoN Regulates Flagellar Synthesis in Stenotrophomonas maltophilia
title_full Interplay between OmpA and RpoN Regulates Flagellar Synthesis in Stenotrophomonas maltophilia
title_fullStr Interplay between OmpA and RpoN Regulates Flagellar Synthesis in Stenotrophomonas maltophilia
title_full_unstemmed Interplay between OmpA and RpoN Regulates Flagellar Synthesis in Stenotrophomonas maltophilia
title_short Interplay between OmpA and RpoN Regulates Flagellar Synthesis in Stenotrophomonas maltophilia
title_sort interplay between ompa and rpon regulates flagellar synthesis in stenotrophomonas maltophilia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8229486/
https://www.ncbi.nlm.nih.gov/pubmed/34199787
http://dx.doi.org/10.3390/microorganisms9061216
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