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Latticed Gold Nanoparticle Conjugation via Monomeric Streptavidin in Lateral Flow Assay for Detection of Autoantibody to Interferon-Gamma

Adult-onset immunodeficiency syndrome (AOID) patients with autoantibodies (autoAbs) against interferon-gamma (IFN-γ) generally suffer from recurrent and recalcitrant disseminated non-tuberculous mycobacterial diseases. Since the early stages of AOID do not present specific symptoms, diagnosis and tr...

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Autores principales: Thongkum, Weeraya, Yasamut, Umpa, Chupradit, Koollawat, Sakkhachornphop, Supachai, Wipasa, Jiraprapa, Sornsuwan, Kanokporn, Juntit, On-anong, Pornprasit, Rawiwan, Thongkamwitoon, Wanwisa, Chaichanan, Jirapan, Khaoplab, Jaruwan, Chanpradab, Chonnikarn, Kasinrerk, Watchara, Tayapiwatana, Chatchai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8229537/
https://www.ncbi.nlm.nih.gov/pubmed/34072539
http://dx.doi.org/10.3390/diagnostics11060987
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author Thongkum, Weeraya
Yasamut, Umpa
Chupradit, Koollawat
Sakkhachornphop, Supachai
Wipasa, Jiraprapa
Sornsuwan, Kanokporn
Juntit, On-anong
Pornprasit, Rawiwan
Thongkamwitoon, Wanwisa
Chaichanan, Jirapan
Khaoplab, Jaruwan
Chanpradab, Chonnikarn
Kasinrerk, Watchara
Tayapiwatana, Chatchai
author_facet Thongkum, Weeraya
Yasamut, Umpa
Chupradit, Koollawat
Sakkhachornphop, Supachai
Wipasa, Jiraprapa
Sornsuwan, Kanokporn
Juntit, On-anong
Pornprasit, Rawiwan
Thongkamwitoon, Wanwisa
Chaichanan, Jirapan
Khaoplab, Jaruwan
Chanpradab, Chonnikarn
Kasinrerk, Watchara
Tayapiwatana, Chatchai
author_sort Thongkum, Weeraya
collection PubMed
description Adult-onset immunodeficiency syndrome (AOID) patients with autoantibodies (autoAbs) against interferon-gamma (IFN-γ) generally suffer from recurrent and recalcitrant disseminated non-tuberculous mycobacterial diseases. Since the early stages of AOID do not present specific symptoms, diagnosis and treatment of the condition are not practical. A simplified diagnostic method for differentiating AOID from other immunodeficiencies, such as HIV infection, was created. Anti-IFN-γ is generally identified using enzyme-linked immunosorbent assay (ELISA), which involves an instrument and a cumbersome process. Recombinant IFN-γ indirectly conjugated to colloidal gold was used in the modified immunochromatographic (IC) strips. The biotinylated-IFN-γ was incorporated with colloidal-gold-labeled 6HIS-maltose binding protein-monomeric streptavidin ((6HIS)MBP-mSA) and absorbed at the conjugate pad. The efficacy of the IC strip upon applying an anti-IFN-γ autoAb cut-off ELISA titer of 2500, the sensitivity and specificity were 84% and 90.24%, respectively. When a cut-off ELISA titer of 500 was applied, the sensitivity and specificity were 73.52% and 100%, respectively.
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spelling pubmed-82295372021-06-26 Latticed Gold Nanoparticle Conjugation via Monomeric Streptavidin in Lateral Flow Assay for Detection of Autoantibody to Interferon-Gamma Thongkum, Weeraya Yasamut, Umpa Chupradit, Koollawat Sakkhachornphop, Supachai Wipasa, Jiraprapa Sornsuwan, Kanokporn Juntit, On-anong Pornprasit, Rawiwan Thongkamwitoon, Wanwisa Chaichanan, Jirapan Khaoplab, Jaruwan Chanpradab, Chonnikarn Kasinrerk, Watchara Tayapiwatana, Chatchai Diagnostics (Basel) Article Adult-onset immunodeficiency syndrome (AOID) patients with autoantibodies (autoAbs) against interferon-gamma (IFN-γ) generally suffer from recurrent and recalcitrant disseminated non-tuberculous mycobacterial diseases. Since the early stages of AOID do not present specific symptoms, diagnosis and treatment of the condition are not practical. A simplified diagnostic method for differentiating AOID from other immunodeficiencies, such as HIV infection, was created. Anti-IFN-γ is generally identified using enzyme-linked immunosorbent assay (ELISA), which involves an instrument and a cumbersome process. Recombinant IFN-γ indirectly conjugated to colloidal gold was used in the modified immunochromatographic (IC) strips. The biotinylated-IFN-γ was incorporated with colloidal-gold-labeled 6HIS-maltose binding protein-monomeric streptavidin ((6HIS)MBP-mSA) and absorbed at the conjugate pad. The efficacy of the IC strip upon applying an anti-IFN-γ autoAb cut-off ELISA titer of 2500, the sensitivity and specificity were 84% and 90.24%, respectively. When a cut-off ELISA titer of 500 was applied, the sensitivity and specificity were 73.52% and 100%, respectively. MDPI 2021-05-29 /pmc/articles/PMC8229537/ /pubmed/34072539 http://dx.doi.org/10.3390/diagnostics11060987 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Thongkum, Weeraya
Yasamut, Umpa
Chupradit, Koollawat
Sakkhachornphop, Supachai
Wipasa, Jiraprapa
Sornsuwan, Kanokporn
Juntit, On-anong
Pornprasit, Rawiwan
Thongkamwitoon, Wanwisa
Chaichanan, Jirapan
Khaoplab, Jaruwan
Chanpradab, Chonnikarn
Kasinrerk, Watchara
Tayapiwatana, Chatchai
Latticed Gold Nanoparticle Conjugation via Monomeric Streptavidin in Lateral Flow Assay for Detection of Autoantibody to Interferon-Gamma
title Latticed Gold Nanoparticle Conjugation via Monomeric Streptavidin in Lateral Flow Assay for Detection of Autoantibody to Interferon-Gamma
title_full Latticed Gold Nanoparticle Conjugation via Monomeric Streptavidin in Lateral Flow Assay for Detection of Autoantibody to Interferon-Gamma
title_fullStr Latticed Gold Nanoparticle Conjugation via Monomeric Streptavidin in Lateral Flow Assay for Detection of Autoantibody to Interferon-Gamma
title_full_unstemmed Latticed Gold Nanoparticle Conjugation via Monomeric Streptavidin in Lateral Flow Assay for Detection of Autoantibody to Interferon-Gamma
title_short Latticed Gold Nanoparticle Conjugation via Monomeric Streptavidin in Lateral Flow Assay for Detection of Autoantibody to Interferon-Gamma
title_sort latticed gold nanoparticle conjugation via monomeric streptavidin in lateral flow assay for detection of autoantibody to interferon-gamma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8229537/
https://www.ncbi.nlm.nih.gov/pubmed/34072539
http://dx.doi.org/10.3390/diagnostics11060987
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