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Potential of Antifungal Proteins (AFPs) to Control Penicillium Postharvest Fruit Decay

Penicillium phytopathogenic species provoke severe postharvest disease and economic losses. Penicillium expansum is the main pome fruit phytopathogen while Penicillium digitatum and Penicillium italicum cause citrus green and blue mold, respectively. Control strategies rely on the use of synthetic f...

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Autores principales: Gandía, Mónica, Kakar, Anant, Giner-Llorca, Moisés, Holzknecht, Jeanett, Martínez-Culebras, Pedro, Galgóczy, László, Marx, Florentine, Marcos, Jose F., Manzanares, Paloma
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8229795/
https://www.ncbi.nlm.nih.gov/pubmed/34199956
http://dx.doi.org/10.3390/jof7060449
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author Gandía, Mónica
Kakar, Anant
Giner-Llorca, Moisés
Holzknecht, Jeanett
Martínez-Culebras, Pedro
Galgóczy, László
Marx, Florentine
Marcos, Jose F.
Manzanares, Paloma
author_facet Gandía, Mónica
Kakar, Anant
Giner-Llorca, Moisés
Holzknecht, Jeanett
Martínez-Culebras, Pedro
Galgóczy, László
Marx, Florentine
Marcos, Jose F.
Manzanares, Paloma
author_sort Gandía, Mónica
collection PubMed
description Penicillium phytopathogenic species provoke severe postharvest disease and economic losses. Penicillium expansum is the main pome fruit phytopathogen while Penicillium digitatum and Penicillium italicum cause citrus green and blue mold, respectively. Control strategies rely on the use of synthetic fungicides, but the appearance of resistant strains and safety concerns have led to the search for new antifungals. Here, the potential application of different antifungal proteins (AFPs) including the three Penicillium chrysogenum proteins (PAF, PAFB and PAFC), as well as the Neosartorya fischeri NFAP2 protein to control Penicillium decay, has been evaluated. PAFB was the most potent AFP against P. digitatum, P. italicum and P. expansum, PAFC and NFAP2 showed moderate antifungal activity, whereas PAF was the least active protein. In fruit protection assays, PAFB provoked a reduction of the incidence of infections caused by P. digitatum and P. italicum in oranges and by P. expansum in apples. A combination of AFPs did not result in an increase in the efficacy of disease control. In conclusion, this study expands the antifungal inhibition spectrum of the AFPs evaluated, and demonstrates that AFPs act in a species-specific manner. PAFB is a promising alternative compound to control Penicillium postharvest fruit decay.
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spelling pubmed-82297952021-06-26 Potential of Antifungal Proteins (AFPs) to Control Penicillium Postharvest Fruit Decay Gandía, Mónica Kakar, Anant Giner-Llorca, Moisés Holzknecht, Jeanett Martínez-Culebras, Pedro Galgóczy, László Marx, Florentine Marcos, Jose F. Manzanares, Paloma J Fungi (Basel) Article Penicillium phytopathogenic species provoke severe postharvest disease and economic losses. Penicillium expansum is the main pome fruit phytopathogen while Penicillium digitatum and Penicillium italicum cause citrus green and blue mold, respectively. Control strategies rely on the use of synthetic fungicides, but the appearance of resistant strains and safety concerns have led to the search for new antifungals. Here, the potential application of different antifungal proteins (AFPs) including the three Penicillium chrysogenum proteins (PAF, PAFB and PAFC), as well as the Neosartorya fischeri NFAP2 protein to control Penicillium decay, has been evaluated. PAFB was the most potent AFP against P. digitatum, P. italicum and P. expansum, PAFC and NFAP2 showed moderate antifungal activity, whereas PAF was the least active protein. In fruit protection assays, PAFB provoked a reduction of the incidence of infections caused by P. digitatum and P. italicum in oranges and by P. expansum in apples. A combination of AFPs did not result in an increase in the efficacy of disease control. In conclusion, this study expands the antifungal inhibition spectrum of the AFPs evaluated, and demonstrates that AFPs act in a species-specific manner. PAFB is a promising alternative compound to control Penicillium postharvest fruit decay. MDPI 2021-06-04 /pmc/articles/PMC8229795/ /pubmed/34199956 http://dx.doi.org/10.3390/jof7060449 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gandía, Mónica
Kakar, Anant
Giner-Llorca, Moisés
Holzknecht, Jeanett
Martínez-Culebras, Pedro
Galgóczy, László
Marx, Florentine
Marcos, Jose F.
Manzanares, Paloma
Potential of Antifungal Proteins (AFPs) to Control Penicillium Postharvest Fruit Decay
title Potential of Antifungal Proteins (AFPs) to Control Penicillium Postharvest Fruit Decay
title_full Potential of Antifungal Proteins (AFPs) to Control Penicillium Postharvest Fruit Decay
title_fullStr Potential of Antifungal Proteins (AFPs) to Control Penicillium Postharvest Fruit Decay
title_full_unstemmed Potential of Antifungal Proteins (AFPs) to Control Penicillium Postharvest Fruit Decay
title_short Potential of Antifungal Proteins (AFPs) to Control Penicillium Postharvest Fruit Decay
title_sort potential of antifungal proteins (afps) to control penicillium postharvest fruit decay
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8229795/
https://www.ncbi.nlm.nih.gov/pubmed/34199956
http://dx.doi.org/10.3390/jof7060449
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