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Anti-Inflammatory, Thrombolytic and Hair-Growth Promoting Activity of the n-Hexane Fraction of the Methanol Extract of Leea indica Leaves
The anti-inflammatory, thrombolytic, and hair growth-promoting activity of the n-hexane fraction from the methanol extract of Leea indica (NFLI) leaves was investigated. NFLI showed significant inhibition of hemolysis and protein denaturation, and exhibited a concentration-dependent thrombolytic act...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8229947/ https://www.ncbi.nlm.nih.gov/pubmed/34072236 http://dx.doi.org/10.3390/plants10061081 |
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author | Sakib, Shahenur Alam Tareq, Abu Montakim Islam, Ameerul Rakib, Ahmed Islam, Mohammad Nazmul Uddin, Mohammad Arafat Rahman, Md. Masudur Seidel, Veronique Emran, Talha Bin |
author_facet | Sakib, Shahenur Alam Tareq, Abu Montakim Islam, Ameerul Rakib, Ahmed Islam, Mohammad Nazmul Uddin, Mohammad Arafat Rahman, Md. Masudur Seidel, Veronique Emran, Talha Bin |
author_sort | Sakib, Shahenur Alam |
collection | PubMed |
description | The anti-inflammatory, thrombolytic, and hair growth-promoting activity of the n-hexane fraction from the methanol extract of Leea indica (NFLI) leaves was investigated. NFLI showed significant inhibition of hemolysis and protein denaturation, and exhibited a concentration-dependent thrombolytic activity. When applied topically to mice at concentrations of 10, 1, 0.1%, NFLI demonstrated a significant increase in average hair length (p < 0.001) compared with untreated animals. NFLI (1% concentration) exhibited the highest percentage of hair regrowth on day 7, 14 and 21 (81.24, 65.60, and 62.5%, respectively). An in silico study was further conducted to predict the binding affinity of phytochemicals previously reported in L. indica towards PGD(2) synthase (PDB ID: 2VD1), an enzyme that catalyses the isomerisation of prostaglandin H(2) to PGD(2) which is involved in hair loss. Phthalic acid, farnesol, n-tricosane, n-tetracosane, and n-heptacosane showed the best ligand efficiencies towards PGD(2) synthase and their intermolecular interactions were visualised using BIOVIA Discovery Studio Visualizer. Our results indicate that L. indica could represent a promising natural alternative to tackle alopecia. |
format | Online Article Text |
id | pubmed-8229947 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-82299472021-06-26 Anti-Inflammatory, Thrombolytic and Hair-Growth Promoting Activity of the n-Hexane Fraction of the Methanol Extract of Leea indica Leaves Sakib, Shahenur Alam Tareq, Abu Montakim Islam, Ameerul Rakib, Ahmed Islam, Mohammad Nazmul Uddin, Mohammad Arafat Rahman, Md. Masudur Seidel, Veronique Emran, Talha Bin Plants (Basel) Article The anti-inflammatory, thrombolytic, and hair growth-promoting activity of the n-hexane fraction from the methanol extract of Leea indica (NFLI) leaves was investigated. NFLI showed significant inhibition of hemolysis and protein denaturation, and exhibited a concentration-dependent thrombolytic activity. When applied topically to mice at concentrations of 10, 1, 0.1%, NFLI demonstrated a significant increase in average hair length (p < 0.001) compared with untreated animals. NFLI (1% concentration) exhibited the highest percentage of hair regrowth on day 7, 14 and 21 (81.24, 65.60, and 62.5%, respectively). An in silico study was further conducted to predict the binding affinity of phytochemicals previously reported in L. indica towards PGD(2) synthase (PDB ID: 2VD1), an enzyme that catalyses the isomerisation of prostaglandin H(2) to PGD(2) which is involved in hair loss. Phthalic acid, farnesol, n-tricosane, n-tetracosane, and n-heptacosane showed the best ligand efficiencies towards PGD(2) synthase and their intermolecular interactions were visualised using BIOVIA Discovery Studio Visualizer. Our results indicate that L. indica could represent a promising natural alternative to tackle alopecia. MDPI 2021-05-27 /pmc/articles/PMC8229947/ /pubmed/34072236 http://dx.doi.org/10.3390/plants10061081 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sakib, Shahenur Alam Tareq, Abu Montakim Islam, Ameerul Rakib, Ahmed Islam, Mohammad Nazmul Uddin, Mohammad Arafat Rahman, Md. Masudur Seidel, Veronique Emran, Talha Bin Anti-Inflammatory, Thrombolytic and Hair-Growth Promoting Activity of the n-Hexane Fraction of the Methanol Extract of Leea indica Leaves |
title | Anti-Inflammatory, Thrombolytic and Hair-Growth Promoting Activity of the n-Hexane Fraction of the Methanol Extract of Leea indica Leaves |
title_full | Anti-Inflammatory, Thrombolytic and Hair-Growth Promoting Activity of the n-Hexane Fraction of the Methanol Extract of Leea indica Leaves |
title_fullStr | Anti-Inflammatory, Thrombolytic and Hair-Growth Promoting Activity of the n-Hexane Fraction of the Methanol Extract of Leea indica Leaves |
title_full_unstemmed | Anti-Inflammatory, Thrombolytic and Hair-Growth Promoting Activity of the n-Hexane Fraction of the Methanol Extract of Leea indica Leaves |
title_short | Anti-Inflammatory, Thrombolytic and Hair-Growth Promoting Activity of the n-Hexane Fraction of the Methanol Extract of Leea indica Leaves |
title_sort | anti-inflammatory, thrombolytic and hair-growth promoting activity of the n-hexane fraction of the methanol extract of leea indica leaves |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8229947/ https://www.ncbi.nlm.nih.gov/pubmed/34072236 http://dx.doi.org/10.3390/plants10061081 |
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