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Diffusible Compounds Produced by Hanseniaspora osmophila and Gluconobacter cerinus Help to Control the Causal Agents of Gray Rot and Summer Bunch Rot of Table Grapes

Gray and summer bunch rot are important diseases of table grapes due to the high economic and environmental cost of their control with synthetic fungicides. The ability to produce antifungal compounds against the causal agents Botrytis, Aspergillus, Penicillium, and Rhizopus of two microorganisms is...

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Autores principales: Olivera, Matías, Delgado, Ninoska, Cádiz, Fabiola, Riquelme, Natalia, Montenegro, Iván, Seeger, Michael, Bravo, Guillermo, Barros-Parada, Wilson, Pedreschi, Romina, Besoain, Ximena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8230015/
https://www.ncbi.nlm.nih.gov/pubmed/34199335
http://dx.doi.org/10.3390/antibiotics10060664
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author Olivera, Matías
Delgado, Ninoska
Cádiz, Fabiola
Riquelme, Natalia
Montenegro, Iván
Seeger, Michael
Bravo, Guillermo
Barros-Parada, Wilson
Pedreschi, Romina
Besoain, Ximena
author_facet Olivera, Matías
Delgado, Ninoska
Cádiz, Fabiola
Riquelme, Natalia
Montenegro, Iván
Seeger, Michael
Bravo, Guillermo
Barros-Parada, Wilson
Pedreschi, Romina
Besoain, Ximena
author_sort Olivera, Matías
collection PubMed
description Gray and summer bunch rot are important diseases of table grapes due to the high economic and environmental cost of their control with synthetic fungicides. The ability to produce antifungal compounds against the causal agents Botrytis, Aspergillus, Penicillium, and Rhizopus of two microorganisms isolated from table grapes and identified as Hanseniaspora osmophila and Gluconobacter cerinus was evaluated. In dual cultures, both biocontrol agents (together and separately) inhibited in vitro mycelial growth of these pathogens. To identify the compounds responsible for the inhibitory effect, extractions were carried out with organic solvents from biocontrol agents separately. Through dual cultures with pathogens and pure extracts, only the hexane extract from H. osmophila showed an inhibitory effect against Botrytis cinerea. To further identify these compounds, the direct bioautography technique was used. This technique made it possible to determine the band displaying antifungal activity at Rf = 0.05–0.2. The compounds present in this band were identified by GC-MS and compared to the NIST library. The most abundant compounds, not previously reported, corresponded to alkanes, ketones, alcohols, and terpenoids. H. osmophila and G. cerinus have the potential to control the causal agents of gray and summer bunch rot of table grapes.
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spelling pubmed-82300152021-06-26 Diffusible Compounds Produced by Hanseniaspora osmophila and Gluconobacter cerinus Help to Control the Causal Agents of Gray Rot and Summer Bunch Rot of Table Grapes Olivera, Matías Delgado, Ninoska Cádiz, Fabiola Riquelme, Natalia Montenegro, Iván Seeger, Michael Bravo, Guillermo Barros-Parada, Wilson Pedreschi, Romina Besoain, Ximena Antibiotics (Basel) Article Gray and summer bunch rot are important diseases of table grapes due to the high economic and environmental cost of their control with synthetic fungicides. The ability to produce antifungal compounds against the causal agents Botrytis, Aspergillus, Penicillium, and Rhizopus of two microorganisms isolated from table grapes and identified as Hanseniaspora osmophila and Gluconobacter cerinus was evaluated. In dual cultures, both biocontrol agents (together and separately) inhibited in vitro mycelial growth of these pathogens. To identify the compounds responsible for the inhibitory effect, extractions were carried out with organic solvents from biocontrol agents separately. Through dual cultures with pathogens and pure extracts, only the hexane extract from H. osmophila showed an inhibitory effect against Botrytis cinerea. To further identify these compounds, the direct bioautography technique was used. This technique made it possible to determine the band displaying antifungal activity at Rf = 0.05–0.2. The compounds present in this band were identified by GC-MS and compared to the NIST library. The most abundant compounds, not previously reported, corresponded to alkanes, ketones, alcohols, and terpenoids. H. osmophila and G. cerinus have the potential to control the causal agents of gray and summer bunch rot of table grapes. MDPI 2021-06-02 /pmc/articles/PMC8230015/ /pubmed/34199335 http://dx.doi.org/10.3390/antibiotics10060664 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Olivera, Matías
Delgado, Ninoska
Cádiz, Fabiola
Riquelme, Natalia
Montenegro, Iván
Seeger, Michael
Bravo, Guillermo
Barros-Parada, Wilson
Pedreschi, Romina
Besoain, Ximena
Diffusible Compounds Produced by Hanseniaspora osmophila and Gluconobacter cerinus Help to Control the Causal Agents of Gray Rot and Summer Bunch Rot of Table Grapes
title Diffusible Compounds Produced by Hanseniaspora osmophila and Gluconobacter cerinus Help to Control the Causal Agents of Gray Rot and Summer Bunch Rot of Table Grapes
title_full Diffusible Compounds Produced by Hanseniaspora osmophila and Gluconobacter cerinus Help to Control the Causal Agents of Gray Rot and Summer Bunch Rot of Table Grapes
title_fullStr Diffusible Compounds Produced by Hanseniaspora osmophila and Gluconobacter cerinus Help to Control the Causal Agents of Gray Rot and Summer Bunch Rot of Table Grapes
title_full_unstemmed Diffusible Compounds Produced by Hanseniaspora osmophila and Gluconobacter cerinus Help to Control the Causal Agents of Gray Rot and Summer Bunch Rot of Table Grapes
title_short Diffusible Compounds Produced by Hanseniaspora osmophila and Gluconobacter cerinus Help to Control the Causal Agents of Gray Rot and Summer Bunch Rot of Table Grapes
title_sort diffusible compounds produced by hanseniaspora osmophila and gluconobacter cerinus help to control the causal agents of gray rot and summer bunch rot of table grapes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8230015/
https://www.ncbi.nlm.nih.gov/pubmed/34199335
http://dx.doi.org/10.3390/antibiotics10060664
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