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Transcriptome Profiling of m(6)A mRNA Modification in Bovine Mammary Epithelial Cells Treated with Escherichia coli
Mastitis is a common disease in dairy cows that is mostly caused by E. coli, and it brings massive losses to the dairy industry. N(6)-Methyladenosine (m(6)A), a methylation at the N(6) position of RNA adenine, is a type of modification strongly associated with many diseases. However, the role of m(6...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8230414/ https://www.ncbi.nlm.nih.gov/pubmed/34200743 http://dx.doi.org/10.3390/ijms22126254 |
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author | Li, Ting Lin, Changjie Zhu, Yifan Xu, Haojun Yin, Yiya Wang, Chaohao Tang, Xin Song, Tongxing Guo, Aizhen Chen, Yingyu Hu, Changmin |
author_facet | Li, Ting Lin, Changjie Zhu, Yifan Xu, Haojun Yin, Yiya Wang, Chaohao Tang, Xin Song, Tongxing Guo, Aizhen Chen, Yingyu Hu, Changmin |
author_sort | Li, Ting |
collection | PubMed |
description | Mastitis is a common disease in dairy cows that is mostly caused by E. coli, and it brings massive losses to the dairy industry. N(6)-Methyladenosine (m(6)A), a methylation at the N(6) position of RNA adenine, is a type of modification strongly associated with many diseases. However, the role of m(6)A in mastitis has not been investigated. In this study, we used MeRIP-seq to sequence the RNA of bovine mammary epithelial cells treated with inactivated E. coli for 24 h. In this in vitro infection model, there were 16,691 m(6)A peaks within 7066 mRNA transcripts in the Con group and 10,029 peaks within 4891 transcripts in the E. coli group. Compared with the Con group, 474 mRNAs were hypermethylated and 2101 mRNAs were hypomethylated in the E. coli group. Biological function analyses revealed differential m(6)A-modified genes mainly enriched in the MAPK, NF-κB, and TGF-β signaling pathways. In order to explore the relationship between m(6)A and mRNA expression, combined MeRIP-seq and mRNA-seq analyses revealed 212 genes with concomitant changes in the mRNA expression and m(6)A modification. This study is the first to present a map of RNA m(6)A modification in mastitis treated with E. coli, providing a basis for future research. |
format | Online Article Text |
id | pubmed-8230414 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-82304142021-06-26 Transcriptome Profiling of m(6)A mRNA Modification in Bovine Mammary Epithelial Cells Treated with Escherichia coli Li, Ting Lin, Changjie Zhu, Yifan Xu, Haojun Yin, Yiya Wang, Chaohao Tang, Xin Song, Tongxing Guo, Aizhen Chen, Yingyu Hu, Changmin Int J Mol Sci Article Mastitis is a common disease in dairy cows that is mostly caused by E. coli, and it brings massive losses to the dairy industry. N(6)-Methyladenosine (m(6)A), a methylation at the N(6) position of RNA adenine, is a type of modification strongly associated with many diseases. However, the role of m(6)A in mastitis has not been investigated. In this study, we used MeRIP-seq to sequence the RNA of bovine mammary epithelial cells treated with inactivated E. coli for 24 h. In this in vitro infection model, there were 16,691 m(6)A peaks within 7066 mRNA transcripts in the Con group and 10,029 peaks within 4891 transcripts in the E. coli group. Compared with the Con group, 474 mRNAs were hypermethylated and 2101 mRNAs were hypomethylated in the E. coli group. Biological function analyses revealed differential m(6)A-modified genes mainly enriched in the MAPK, NF-κB, and TGF-β signaling pathways. In order to explore the relationship between m(6)A and mRNA expression, combined MeRIP-seq and mRNA-seq analyses revealed 212 genes with concomitant changes in the mRNA expression and m(6)A modification. This study is the first to present a map of RNA m(6)A modification in mastitis treated with E. coli, providing a basis for future research. MDPI 2021-06-10 /pmc/articles/PMC8230414/ /pubmed/34200743 http://dx.doi.org/10.3390/ijms22126254 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Li, Ting Lin, Changjie Zhu, Yifan Xu, Haojun Yin, Yiya Wang, Chaohao Tang, Xin Song, Tongxing Guo, Aizhen Chen, Yingyu Hu, Changmin Transcriptome Profiling of m(6)A mRNA Modification in Bovine Mammary Epithelial Cells Treated with Escherichia coli |
title | Transcriptome Profiling of m(6)A mRNA Modification in Bovine Mammary Epithelial Cells Treated with Escherichia coli |
title_full | Transcriptome Profiling of m(6)A mRNA Modification in Bovine Mammary Epithelial Cells Treated with Escherichia coli |
title_fullStr | Transcriptome Profiling of m(6)A mRNA Modification in Bovine Mammary Epithelial Cells Treated with Escherichia coli |
title_full_unstemmed | Transcriptome Profiling of m(6)A mRNA Modification in Bovine Mammary Epithelial Cells Treated with Escherichia coli |
title_short | Transcriptome Profiling of m(6)A mRNA Modification in Bovine Mammary Epithelial Cells Treated with Escherichia coli |
title_sort | transcriptome profiling of m(6)a mrna modification in bovine mammary epithelial cells treated with escherichia coli |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8230414/ https://www.ncbi.nlm.nih.gov/pubmed/34200743 http://dx.doi.org/10.3390/ijms22126254 |
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