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Influence of GdVO(4):Eu(3+) Nanocrystals on Growth, Germination, Root Cell Viability and Oxidative Stress of Wheat (Triticum aestivum L.) Seedlings
The increasing application of lanthanide-doped nanocrystals (LDNCs) entails the risk of a harmful impact on the natural environment. Therefore, in the presented study the influence of gadolinium orthovanadates doped with Eu(3+) (GdVO(4):Eu(3)) nanocrystals on wheat (Triticum aestivum L.), chosen as...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8230434/ https://www.ncbi.nlm.nih.gov/pubmed/34200921 http://dx.doi.org/10.3390/plants10061187 |
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author | Ekner-Grzyb, Anna Chmielowska-Bąk, Jagna Szczeszak, Agata |
author_facet | Ekner-Grzyb, Anna Chmielowska-Bąk, Jagna Szczeszak, Agata |
author_sort | Ekner-Grzyb, Anna |
collection | PubMed |
description | The increasing application of lanthanide-doped nanocrystals (LDNCs) entails the risk of a harmful impact on the natural environment. Therefore, in the presented study the influence of gadolinium orthovanadates doped with Eu(3+) (GdVO(4):Eu(3)) nanocrystals on wheat (Triticum aestivum L.), chosen as a model plant species, was investigated. The seeds were grown in Petri dishes filled with colloids of LDNCs at the concentrations of 0, 10, 50 and 100 µg/mL. The plants’ growth endpoints (number of roots, roots length, roots mass, hypocotyl length and hypocotyl mass) and germination rate were not significantly changed after the exposure to GdVO(4):Eu(3+) nanocrystals at all used concentrations. The presence of LDNCs also had no effect on oxidative stress intensity, which was determined on the basis of the amount of lipid peroxidation product (thiobarbituric acid reactive substances; TBARS) in the roots. Similarly, TTC (tetrazolium chloride) assay did not show any differences in cells’ viability. However, root cells of the treated seedlings contained less Evans Blue (EB) when compared to the control. The obtained results, on the one hand, suggest that GdVO(4):Eu(3+) nanocrystals are safe for plants in the tested concentrations, while on the other hand they indicate that LDNCs may interfere with the functioning of the root cell membrane. |
format | Online Article Text |
id | pubmed-8230434 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-82304342021-06-26 Influence of GdVO(4):Eu(3+) Nanocrystals on Growth, Germination, Root Cell Viability and Oxidative Stress of Wheat (Triticum aestivum L.) Seedlings Ekner-Grzyb, Anna Chmielowska-Bąk, Jagna Szczeszak, Agata Plants (Basel) Article The increasing application of lanthanide-doped nanocrystals (LDNCs) entails the risk of a harmful impact on the natural environment. Therefore, in the presented study the influence of gadolinium orthovanadates doped with Eu(3+) (GdVO(4):Eu(3)) nanocrystals on wheat (Triticum aestivum L.), chosen as a model plant species, was investigated. The seeds were grown in Petri dishes filled with colloids of LDNCs at the concentrations of 0, 10, 50 and 100 µg/mL. The plants’ growth endpoints (number of roots, roots length, roots mass, hypocotyl length and hypocotyl mass) and germination rate were not significantly changed after the exposure to GdVO(4):Eu(3+) nanocrystals at all used concentrations. The presence of LDNCs also had no effect on oxidative stress intensity, which was determined on the basis of the amount of lipid peroxidation product (thiobarbituric acid reactive substances; TBARS) in the roots. Similarly, TTC (tetrazolium chloride) assay did not show any differences in cells’ viability. However, root cells of the treated seedlings contained less Evans Blue (EB) when compared to the control. The obtained results, on the one hand, suggest that GdVO(4):Eu(3+) nanocrystals are safe for plants in the tested concentrations, while on the other hand they indicate that LDNCs may interfere with the functioning of the root cell membrane. MDPI 2021-06-10 /pmc/articles/PMC8230434/ /pubmed/34200921 http://dx.doi.org/10.3390/plants10061187 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ekner-Grzyb, Anna Chmielowska-Bąk, Jagna Szczeszak, Agata Influence of GdVO(4):Eu(3+) Nanocrystals on Growth, Germination, Root Cell Viability and Oxidative Stress of Wheat (Triticum aestivum L.) Seedlings |
title | Influence of GdVO(4):Eu(3+) Nanocrystals on Growth, Germination, Root Cell Viability and Oxidative Stress of Wheat (Triticum aestivum L.) Seedlings |
title_full | Influence of GdVO(4):Eu(3+) Nanocrystals on Growth, Germination, Root Cell Viability and Oxidative Stress of Wheat (Triticum aestivum L.) Seedlings |
title_fullStr | Influence of GdVO(4):Eu(3+) Nanocrystals on Growth, Germination, Root Cell Viability and Oxidative Stress of Wheat (Triticum aestivum L.) Seedlings |
title_full_unstemmed | Influence of GdVO(4):Eu(3+) Nanocrystals on Growth, Germination, Root Cell Viability and Oxidative Stress of Wheat (Triticum aestivum L.) Seedlings |
title_short | Influence of GdVO(4):Eu(3+) Nanocrystals on Growth, Germination, Root Cell Viability and Oxidative Stress of Wheat (Triticum aestivum L.) Seedlings |
title_sort | influence of gdvo(4):eu(3+) nanocrystals on growth, germination, root cell viability and oxidative stress of wheat (triticum aestivum l.) seedlings |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8230434/ https://www.ncbi.nlm.nih.gov/pubmed/34200921 http://dx.doi.org/10.3390/plants10061187 |
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