Historical Changes in Honey Bee Wing Venation in Romania

SIMPLE SUMMARY: Honey bees, in addition to producing honey, are important pollinators of wild and cultivated plants. Unfortunately, in some places, the population of honey bees is declining. One of the factors that affect their survival is adaptation to the local environment. Bees native to a particular area survive better than those imported. Despite this fact, some beekeepers import non-native bees and use them in their apiaries. Imported bees produce hybrids with bees from surrounding colonies because beekeepers do not control their mating. In consequence, the whole population can change. In this study, we verified how the population of Romanian bees has changed over the last four decades. We found significant temporal changes in wing venation. Despite these changes, the two major subpopulations of bees separated by mountains remain distinct. We provide a tool for the easy identification of native bees from Romania, which can help to protect them. ABSTRACT: The honey bee (Apis mellifera) is an ecologically and economically important species that provides pollination services to natural and agricultural systems. The biodiversity of the honey bee is being endangered by the mass import of non-native queens. In many locations, it is not clear how the local populations have been affected by hybridisation between native and non-native bees. There is especially little information about temporal changes in hybridisation. In Romania, A. m. carpatica naturally occurs, and earlier studies show that there are two subpopulations separated by the Carpathian Mountains. In this study, we investigated how the arrangement of veins in bees’ wings (venation) has changed in Romanian honey bees in the last four decades. We found that in the contemporary population of Romanian bees, there are still clear differences between the intra- and extra-Carpathian subpopulations, which indicates that natural variation among honey bees is still being preserved. We also found significant differences between bees collected before and after 2000. The observed temporal changes in wing venation are most likely caused by hybridisation between native bees and non-native bees sporadically introduced by beekeepers. In order to facilitate conservation and the monitoring of native Romanian bees, we developed a method facilitating their identification.