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Dynamics of Diversity and Abundance of Sulfonamide Resistant Bacteria in a Silt Loam Soil Fertilized by Compost

Although composting is effective in deactivating antibiotic substances in manure, the influence of compost fertilization on the occurrence and dissemination of antibiotic resistance in arable soils remains to be controversial. Herein, the abundance and diversity of two sulfonamide resistance genes (...

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Autores principales: Han, Hui, Bai, Mohan, Chen, Yanting, Gong, Yali, Wu, Ming, Yang, Hefa, Chen, Qing, Xu, Ting, Wei, Yuquan, Ding, Guochun, Li, Ji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8230599/
https://www.ncbi.nlm.nih.gov/pubmed/34207996
http://dx.doi.org/10.3390/antibiotics10060699
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author Han, Hui
Bai, Mohan
Chen, Yanting
Gong, Yali
Wu, Ming
Yang, Hefa
Chen, Qing
Xu, Ting
Wei, Yuquan
Ding, Guochun
Li, Ji
author_facet Han, Hui
Bai, Mohan
Chen, Yanting
Gong, Yali
Wu, Ming
Yang, Hefa
Chen, Qing
Xu, Ting
Wei, Yuquan
Ding, Guochun
Li, Ji
author_sort Han, Hui
collection PubMed
description Although composting is effective in deactivating antibiotic substances in manure, the influence of compost fertilization on the occurrence and dissemination of antibiotic resistance in arable soils remains to be controversial. Herein, the abundance and diversity of two sulfonamide resistance genes (sul1 and sul2) in soil fertilized by compost spiked with two concentrations of sulfadiazine (1 and 10 mg kg(−1)) were studied intensively by qPCR and high throughput sequencing based on a two-month microcosm experiment. The concentration of sulfadiazine decreased rapidly after spiking from 25% at Day 1 to less than 2.7% at Day 60. Relative abundance of both sul1 and sul2 were significantly higher in soil amended with compost than the non-amended control at Day 1 and slightly decreased with incubation time except for sul2 in the S10 treatment. Soil bacterial communities were transiently shifted by compost fertilization regardless of the presence of sulfadiazine. Relative abundance of genera in three hubs positively interlinked with sul1 and sul2 were significantly higher in compost treated soil than the control at Day 1, 7 and 21, but not at Day 60. High throughput sequencing analyses revealed that most detected (>67% in relative abundance) sul1 and sul2 genotypes sharing >99% similarity with those found in gammaproteobacterial pathogens frequently were commonly present in compost and soil. These results indicated that compost fertilization might increase the abundance rather than diversity of sulfadiazine-resistant populations in soil, which may be facilitated by the presence of sulfadiazine.
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spelling pubmed-82305992021-06-26 Dynamics of Diversity and Abundance of Sulfonamide Resistant Bacteria in a Silt Loam Soil Fertilized by Compost Han, Hui Bai, Mohan Chen, Yanting Gong, Yali Wu, Ming Yang, Hefa Chen, Qing Xu, Ting Wei, Yuquan Ding, Guochun Li, Ji Antibiotics (Basel) Article Although composting is effective in deactivating antibiotic substances in manure, the influence of compost fertilization on the occurrence and dissemination of antibiotic resistance in arable soils remains to be controversial. Herein, the abundance and diversity of two sulfonamide resistance genes (sul1 and sul2) in soil fertilized by compost spiked with two concentrations of sulfadiazine (1 and 10 mg kg(−1)) were studied intensively by qPCR and high throughput sequencing based on a two-month microcosm experiment. The concentration of sulfadiazine decreased rapidly after spiking from 25% at Day 1 to less than 2.7% at Day 60. Relative abundance of both sul1 and sul2 were significantly higher in soil amended with compost than the non-amended control at Day 1 and slightly decreased with incubation time except for sul2 in the S10 treatment. Soil bacterial communities were transiently shifted by compost fertilization regardless of the presence of sulfadiazine. Relative abundance of genera in three hubs positively interlinked with sul1 and sul2 were significantly higher in compost treated soil than the control at Day 1, 7 and 21, but not at Day 60. High throughput sequencing analyses revealed that most detected (>67% in relative abundance) sul1 and sul2 genotypes sharing >99% similarity with those found in gammaproteobacterial pathogens frequently were commonly present in compost and soil. These results indicated that compost fertilization might increase the abundance rather than diversity of sulfadiazine-resistant populations in soil, which may be facilitated by the presence of sulfadiazine. MDPI 2021-06-11 /pmc/articles/PMC8230599/ /pubmed/34207996 http://dx.doi.org/10.3390/antibiotics10060699 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Han, Hui
Bai, Mohan
Chen, Yanting
Gong, Yali
Wu, Ming
Yang, Hefa
Chen, Qing
Xu, Ting
Wei, Yuquan
Ding, Guochun
Li, Ji
Dynamics of Diversity and Abundance of Sulfonamide Resistant Bacteria in a Silt Loam Soil Fertilized by Compost
title Dynamics of Diversity and Abundance of Sulfonamide Resistant Bacteria in a Silt Loam Soil Fertilized by Compost
title_full Dynamics of Diversity and Abundance of Sulfonamide Resistant Bacteria in a Silt Loam Soil Fertilized by Compost
title_fullStr Dynamics of Diversity and Abundance of Sulfonamide Resistant Bacteria in a Silt Loam Soil Fertilized by Compost
title_full_unstemmed Dynamics of Diversity and Abundance of Sulfonamide Resistant Bacteria in a Silt Loam Soil Fertilized by Compost
title_short Dynamics of Diversity and Abundance of Sulfonamide Resistant Bacteria in a Silt Loam Soil Fertilized by Compost
title_sort dynamics of diversity and abundance of sulfonamide resistant bacteria in a silt loam soil fertilized by compost
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8230599/
https://www.ncbi.nlm.nih.gov/pubmed/34207996
http://dx.doi.org/10.3390/antibiotics10060699
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