Cargando…
Interplay of Rice Stripe Virus and Rice Black Streaked Dwarf Virus during Their Acquisition and Accumulation in Insect Vector
Plant viruses transmitted by hemipteran vectors commonly cause losses to crop production. Rice stripe virus (RSV) and rice black streaked dwarf virus (RBSDV) are transmitted to rice plants by the same vector, the small brown planthopper (SBPH), Laodelphax striatellus Fallén, in a persistent propagat...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8230606/ https://www.ncbi.nlm.nih.gov/pubmed/34200968 http://dx.doi.org/10.3390/v13061121 |
_version_ | 1783713250609725440 |
---|---|
author | Moya Fernández, Marcia Beatriz Liu, Wenwen Zhang, Lu Hajano, Jamal-U-Ddin Wang, Xifeng |
author_facet | Moya Fernández, Marcia Beatriz Liu, Wenwen Zhang, Lu Hajano, Jamal-U-Ddin Wang, Xifeng |
author_sort | Moya Fernández, Marcia Beatriz |
collection | PubMed |
description | Plant viruses transmitted by hemipteran vectors commonly cause losses to crop production. Rice stripe virus (RSV) and rice black streaked dwarf virus (RBSDV) are transmitted to rice plants by the same vector, the small brown planthopper (SBPH), Laodelphax striatellus Fallén, in a persistent propagative manner. However, rarely do the respective diseases they cause occur simultaneously in a field. Here, we determined the acquisition efficiency of RSV and RBSDV when acquired in succession or simultaneously by SBPH. When RBSDV was acquired first, RSV acquisition efficiency was significantly lower than when only acquiring RSV. However, RBSDV acquisition efficiency from insects that acquired RSV first was not significantly different between the insects only acquiring RBSDV. Immunofluorescence assays showed that the acquisition of RBSDV first might inhibit RSV entry into midgut epithelial cells, but RSV did not affect RBSDV entry. SBPHs were more likely to acquire RBSDV when they were feeding on plants coinfected with the two viruses. When RBSDV was acquired before RSV, RBSDV titer was significantly higher and RSV titer first declined, then increased compared to when only acquiring RBSDV or RSV. Only 5% of the SBPHs acquired both viruses when feeding on plants coinfected with RSV and RBSDV. These results provide a better understanding of the interaction between two persistent viruses when present in the same vector insect and explain why RSV and RBSDV occur in intermittent epidemics. |
format | Online Article Text |
id | pubmed-8230606 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-82306062021-06-26 Interplay of Rice Stripe Virus and Rice Black Streaked Dwarf Virus during Their Acquisition and Accumulation in Insect Vector Moya Fernández, Marcia Beatriz Liu, Wenwen Zhang, Lu Hajano, Jamal-U-Ddin Wang, Xifeng Viruses Article Plant viruses transmitted by hemipteran vectors commonly cause losses to crop production. Rice stripe virus (RSV) and rice black streaked dwarf virus (RBSDV) are transmitted to rice plants by the same vector, the small brown planthopper (SBPH), Laodelphax striatellus Fallén, in a persistent propagative manner. However, rarely do the respective diseases they cause occur simultaneously in a field. Here, we determined the acquisition efficiency of RSV and RBSDV when acquired in succession or simultaneously by SBPH. When RBSDV was acquired first, RSV acquisition efficiency was significantly lower than when only acquiring RSV. However, RBSDV acquisition efficiency from insects that acquired RSV first was not significantly different between the insects only acquiring RBSDV. Immunofluorescence assays showed that the acquisition of RBSDV first might inhibit RSV entry into midgut epithelial cells, but RSV did not affect RBSDV entry. SBPHs were more likely to acquire RBSDV when they were feeding on plants coinfected with the two viruses. When RBSDV was acquired before RSV, RBSDV titer was significantly higher and RSV titer first declined, then increased compared to when only acquiring RBSDV or RSV. Only 5% of the SBPHs acquired both viruses when feeding on plants coinfected with RSV and RBSDV. These results provide a better understanding of the interaction between two persistent viruses when present in the same vector insect and explain why RSV and RBSDV occur in intermittent epidemics. MDPI 2021-06-10 /pmc/articles/PMC8230606/ /pubmed/34200968 http://dx.doi.org/10.3390/v13061121 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Moya Fernández, Marcia Beatriz Liu, Wenwen Zhang, Lu Hajano, Jamal-U-Ddin Wang, Xifeng Interplay of Rice Stripe Virus and Rice Black Streaked Dwarf Virus during Their Acquisition and Accumulation in Insect Vector |
title | Interplay of Rice Stripe Virus and Rice Black Streaked Dwarf Virus during Their Acquisition and Accumulation in Insect Vector |
title_full | Interplay of Rice Stripe Virus and Rice Black Streaked Dwarf Virus during Their Acquisition and Accumulation in Insect Vector |
title_fullStr | Interplay of Rice Stripe Virus and Rice Black Streaked Dwarf Virus during Their Acquisition and Accumulation in Insect Vector |
title_full_unstemmed | Interplay of Rice Stripe Virus and Rice Black Streaked Dwarf Virus during Their Acquisition and Accumulation in Insect Vector |
title_short | Interplay of Rice Stripe Virus and Rice Black Streaked Dwarf Virus during Their Acquisition and Accumulation in Insect Vector |
title_sort | interplay of rice stripe virus and rice black streaked dwarf virus during their acquisition and accumulation in insect vector |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8230606/ https://www.ncbi.nlm.nih.gov/pubmed/34200968 http://dx.doi.org/10.3390/v13061121 |
work_keys_str_mv | AT moyafernandezmarciabeatriz interplayofricestripevirusandriceblackstreakeddwarfvirusduringtheiracquisitionandaccumulationininsectvector AT liuwenwen interplayofricestripevirusandriceblackstreakeddwarfvirusduringtheiracquisitionandaccumulationininsectvector AT zhanglu interplayofricestripevirusandriceblackstreakeddwarfvirusduringtheiracquisitionandaccumulationininsectvector AT hajanojamaluddin interplayofricestripevirusandriceblackstreakeddwarfvirusduringtheiracquisitionandaccumulationininsectvector AT wangxifeng interplayofricestripevirusandriceblackstreakeddwarfvirusduringtheiracquisitionandaccumulationininsectvector |