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Strong Antimicrobial and Healing Effects of Beta-Acids from Hops in Methicillin-Resistant Staphylococcus aureus-Infected External Wounds In Vivo

Staphylococcus (S.) aureus is an important causative agent of wound infections with increasing incidence in the past decades. Specifically, the emergence of methicillin-resistant S. aureus (MRSA) causes serious problems, especially in nosocomial infections. Therefore, there is an urgent need to deve...

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Autores principales: Sleha, Radek, Radochova, Vera, Malis, Jiri, Mikyska, Alexander, Houska, Milan, Krofta, Karel, Bogdanova, Katerina, Janovska, Sylva, Pejchal, Jaroslav, Kolar, Milan, Cermak, Pavel, Bostik, Pavel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8231114/
https://www.ncbi.nlm.nih.gov/pubmed/34204644
http://dx.doi.org/10.3390/antibiotics10060708
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author Sleha, Radek
Radochova, Vera
Malis, Jiri
Mikyska, Alexander
Houska, Milan
Krofta, Karel
Bogdanova, Katerina
Janovska, Sylva
Pejchal, Jaroslav
Kolar, Milan
Cermak, Pavel
Bostik, Pavel
author_facet Sleha, Radek
Radochova, Vera
Malis, Jiri
Mikyska, Alexander
Houska, Milan
Krofta, Karel
Bogdanova, Katerina
Janovska, Sylva
Pejchal, Jaroslav
Kolar, Milan
Cermak, Pavel
Bostik, Pavel
author_sort Sleha, Radek
collection PubMed
description Staphylococcus (S.) aureus is an important causative agent of wound infections with increasing incidence in the past decades. Specifically, the emergence of methicillin-resistant S. aureus (MRSA) causes serious problems, especially in nosocomial infections. Therefore, there is an urgent need to develop of alternative or supportive antimicrobial therapeutic modalities to meet these challenges. Purified compounds from hops have previously shown promising antimicrobial effects against MRSA isolates in vitro. In this study, purified beta-acids from hops were tested for their potential antimicrobial and healing properties using a porcine model of wounds infected by MRSA. The results show highly significant antimicrobial effects of the active substance in both the powder and Ambiderman-based application forms compared to both no-treatment control and treatment with Framycoin. Moreover, the macroscopic evaluation of the wounds during the treatment using the standardized Wound Healing Continuum indicated positive effects of the beta-acids on the overall wound healing. This is further supported by the microscopic data, which showed a clear improvement of the inflammatory parameters in the wounds treated by beta-acids. Thus, using the porcine model, we demonstrate significant therapeutic effects of hops compounds in the management of wounds infected by MRSA. Beta-acids from hops, therefore, represent a suitable candidate for the treatment of non-responsive nosocomial tissue infections by MRSA.
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spelling pubmed-82311142021-06-26 Strong Antimicrobial and Healing Effects of Beta-Acids from Hops in Methicillin-Resistant Staphylococcus aureus-Infected External Wounds In Vivo Sleha, Radek Radochova, Vera Malis, Jiri Mikyska, Alexander Houska, Milan Krofta, Karel Bogdanova, Katerina Janovska, Sylva Pejchal, Jaroslav Kolar, Milan Cermak, Pavel Bostik, Pavel Antibiotics (Basel) Article Staphylococcus (S.) aureus is an important causative agent of wound infections with increasing incidence in the past decades. Specifically, the emergence of methicillin-resistant S. aureus (MRSA) causes serious problems, especially in nosocomial infections. Therefore, there is an urgent need to develop of alternative or supportive antimicrobial therapeutic modalities to meet these challenges. Purified compounds from hops have previously shown promising antimicrobial effects against MRSA isolates in vitro. In this study, purified beta-acids from hops were tested for their potential antimicrobial and healing properties using a porcine model of wounds infected by MRSA. The results show highly significant antimicrobial effects of the active substance in both the powder and Ambiderman-based application forms compared to both no-treatment control and treatment with Framycoin. Moreover, the macroscopic evaluation of the wounds during the treatment using the standardized Wound Healing Continuum indicated positive effects of the beta-acids on the overall wound healing. This is further supported by the microscopic data, which showed a clear improvement of the inflammatory parameters in the wounds treated by beta-acids. Thus, using the porcine model, we demonstrate significant therapeutic effects of hops compounds in the management of wounds infected by MRSA. Beta-acids from hops, therefore, represent a suitable candidate for the treatment of non-responsive nosocomial tissue infections by MRSA. MDPI 2021-06-12 /pmc/articles/PMC8231114/ /pubmed/34204644 http://dx.doi.org/10.3390/antibiotics10060708 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sleha, Radek
Radochova, Vera
Malis, Jiri
Mikyska, Alexander
Houska, Milan
Krofta, Karel
Bogdanova, Katerina
Janovska, Sylva
Pejchal, Jaroslav
Kolar, Milan
Cermak, Pavel
Bostik, Pavel
Strong Antimicrobial and Healing Effects of Beta-Acids from Hops in Methicillin-Resistant Staphylococcus aureus-Infected External Wounds In Vivo
title Strong Antimicrobial and Healing Effects of Beta-Acids from Hops in Methicillin-Resistant Staphylococcus aureus-Infected External Wounds In Vivo
title_full Strong Antimicrobial and Healing Effects of Beta-Acids from Hops in Methicillin-Resistant Staphylococcus aureus-Infected External Wounds In Vivo
title_fullStr Strong Antimicrobial and Healing Effects of Beta-Acids from Hops in Methicillin-Resistant Staphylococcus aureus-Infected External Wounds In Vivo
title_full_unstemmed Strong Antimicrobial and Healing Effects of Beta-Acids from Hops in Methicillin-Resistant Staphylococcus aureus-Infected External Wounds In Vivo
title_short Strong Antimicrobial and Healing Effects of Beta-Acids from Hops in Methicillin-Resistant Staphylococcus aureus-Infected External Wounds In Vivo
title_sort strong antimicrobial and healing effects of beta-acids from hops in methicillin-resistant staphylococcus aureus-infected external wounds in vivo
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8231114/
https://www.ncbi.nlm.nih.gov/pubmed/34204644
http://dx.doi.org/10.3390/antibiotics10060708
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