Cargando…
Isoflavonoid-Antibiotic Thin Films Fabricated by MAPLE with Improved Resistance to Microbial Colonization
Staphylococcus aureus (Gram-positive) and Pseudomonas aeruginosa (Gram-negative) bacteria represent major infectious threats in the hospital environment due to their wide distribution, opportunistic behavior, and increasing antibiotic resistance. This study reports on the deposition of polyvinylpyrr...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8231875/ https://www.ncbi.nlm.nih.gov/pubmed/34198596 http://dx.doi.org/10.3390/molecules26123634 |
_version_ | 1783713515200053248 |
---|---|
author | Grumezescu, Valentina Negut, Irina Cristescu, Rodica Grumezescu, Alexandru Mihai Holban, Alina Maria Iordache, Florin Chifiriuc, Mariana Carmen Narayan, Roger J. Chrisey, Douglas B. |
author_facet | Grumezescu, Valentina Negut, Irina Cristescu, Rodica Grumezescu, Alexandru Mihai Holban, Alina Maria Iordache, Florin Chifiriuc, Mariana Carmen Narayan, Roger J. Chrisey, Douglas B. |
author_sort | Grumezescu, Valentina |
collection | PubMed |
description | Staphylococcus aureus (Gram-positive) and Pseudomonas aeruginosa (Gram-negative) bacteria represent major infectious threats in the hospital environment due to their wide distribution, opportunistic behavior, and increasing antibiotic resistance. This study reports on the deposition of polyvinylpyrrolidone/antibiotic/isoflavonoid thin films by the matrix-assisted pulsed laser evaporation (MAPLE) method as anti-adhesion barrier coatings, on biomedical surfaces for improved resistance to microbial colonization. The thin films were characterized by Fourier transform infrared spectroscopy, infrared microscopy, and scanning electron microscopy. In vitro biological assay tests were performed to evaluate the influence of the thin films on the development of biofilms formed by Gram-positive and Gram-negative bacterial strains. In vitro biocompatibility tests were assessed on human endothelial cells examined for up to five days of incubation, via qualitative and quantitative methods. The results of this study revealed that the laser-fabricated coatings are biocompatible and resistant to microbial colonization and biofilm formation, making them successful candidates for biomedical devices and contact surfaces that would otherwise be amenable to contact transmission. |
format | Online Article Text |
id | pubmed-8231875 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-82318752021-06-26 Isoflavonoid-Antibiotic Thin Films Fabricated by MAPLE with Improved Resistance to Microbial Colonization Grumezescu, Valentina Negut, Irina Cristescu, Rodica Grumezescu, Alexandru Mihai Holban, Alina Maria Iordache, Florin Chifiriuc, Mariana Carmen Narayan, Roger J. Chrisey, Douglas B. Molecules Article Staphylococcus aureus (Gram-positive) and Pseudomonas aeruginosa (Gram-negative) bacteria represent major infectious threats in the hospital environment due to their wide distribution, opportunistic behavior, and increasing antibiotic resistance. This study reports on the deposition of polyvinylpyrrolidone/antibiotic/isoflavonoid thin films by the matrix-assisted pulsed laser evaporation (MAPLE) method as anti-adhesion barrier coatings, on biomedical surfaces for improved resistance to microbial colonization. The thin films were characterized by Fourier transform infrared spectroscopy, infrared microscopy, and scanning electron microscopy. In vitro biological assay tests were performed to evaluate the influence of the thin films on the development of biofilms formed by Gram-positive and Gram-negative bacterial strains. In vitro biocompatibility tests were assessed on human endothelial cells examined for up to five days of incubation, via qualitative and quantitative methods. The results of this study revealed that the laser-fabricated coatings are biocompatible and resistant to microbial colonization and biofilm formation, making them successful candidates for biomedical devices and contact surfaces that would otherwise be amenable to contact transmission. MDPI 2021-06-14 /pmc/articles/PMC8231875/ /pubmed/34198596 http://dx.doi.org/10.3390/molecules26123634 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Grumezescu, Valentina Negut, Irina Cristescu, Rodica Grumezescu, Alexandru Mihai Holban, Alina Maria Iordache, Florin Chifiriuc, Mariana Carmen Narayan, Roger J. Chrisey, Douglas B. Isoflavonoid-Antibiotic Thin Films Fabricated by MAPLE with Improved Resistance to Microbial Colonization |
title | Isoflavonoid-Antibiotic Thin Films Fabricated by MAPLE with Improved Resistance to Microbial Colonization |
title_full | Isoflavonoid-Antibiotic Thin Films Fabricated by MAPLE with Improved Resistance to Microbial Colonization |
title_fullStr | Isoflavonoid-Antibiotic Thin Films Fabricated by MAPLE with Improved Resistance to Microbial Colonization |
title_full_unstemmed | Isoflavonoid-Antibiotic Thin Films Fabricated by MAPLE with Improved Resistance to Microbial Colonization |
title_short | Isoflavonoid-Antibiotic Thin Films Fabricated by MAPLE with Improved Resistance to Microbial Colonization |
title_sort | isoflavonoid-antibiotic thin films fabricated by maple with improved resistance to microbial colonization |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8231875/ https://www.ncbi.nlm.nih.gov/pubmed/34198596 http://dx.doi.org/10.3390/molecules26123634 |
work_keys_str_mv | AT grumezescuvalentina isoflavonoidantibioticthinfilmsfabricatedbymaplewithimprovedresistancetomicrobialcolonization AT negutirina isoflavonoidantibioticthinfilmsfabricatedbymaplewithimprovedresistancetomicrobialcolonization AT cristescurodica isoflavonoidantibioticthinfilmsfabricatedbymaplewithimprovedresistancetomicrobialcolonization AT grumezescualexandrumihai isoflavonoidantibioticthinfilmsfabricatedbymaplewithimprovedresistancetomicrobialcolonization AT holbanalinamaria isoflavonoidantibioticthinfilmsfabricatedbymaplewithimprovedresistancetomicrobialcolonization AT iordacheflorin isoflavonoidantibioticthinfilmsfabricatedbymaplewithimprovedresistancetomicrobialcolonization AT chifiriucmarianacarmen isoflavonoidantibioticthinfilmsfabricatedbymaplewithimprovedresistancetomicrobialcolonization AT narayanrogerj isoflavonoidantibioticthinfilmsfabricatedbymaplewithimprovedresistancetomicrobialcolonization AT chriseydouglasb isoflavonoidantibioticthinfilmsfabricatedbymaplewithimprovedresistancetomicrobialcolonization |