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The Biotin–Avidin Interaction in Biotinylated Gold Nanoparticles and the Modulation of Their Aggregation

The biotin–avidin interaction is used as a binding tool for the conjugation of biomolecules for more diverse applications; these include nanoparticle conjugation. Despite this, a thorough investigation on the different aggregates that may result from the interaction of biotinylated nanoparticles (go...

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Autores principales: Lyu, Yanchao, Martínez, Álvaro, D’Incà, Federica, Mancin, Fabrizio, Scrimin, Paolo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8231960/
https://www.ncbi.nlm.nih.gov/pubmed/34199307
http://dx.doi.org/10.3390/nano11061559
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author Lyu, Yanchao
Martínez, Álvaro
D’Incà, Federica
Mancin, Fabrizio
Scrimin, Paolo
author_facet Lyu, Yanchao
Martínez, Álvaro
D’Incà, Federica
Mancin, Fabrizio
Scrimin, Paolo
author_sort Lyu, Yanchao
collection PubMed
description The biotin–avidin interaction is used as a binding tool for the conjugation of biomolecules for more diverse applications; these include nanoparticle conjugation. Despite this, a thorough investigation on the different aggregates that may result from the interaction of biotinylated nanoparticles (gold nanoparticles, AuNPs, in this work) with avidin has not been carried out so far. In this paper, we address this problem and show the type of aggregates formed under thermodynamic and kinetic control by varying the biotinylated AuNP/avidin ratio and the order of addition of the two partners. The analysis was performed by also addressing the amount of protein able to interact with the AuNPs surface and is fully supported by the TEM images collected for the different samples and the shift of the surface plasmon resonance band. We show that the percentage of saturation depends on the size of the nanoparticles, and larger nanoparticles (19 nm in diameter) manage to accommodate a relatively larger amount of avidins than smaller ones (11 nm). The AuNPs are isolated or form small clusters (mostly dimers or trimers) when a large excess or a very low amount of avidin is present, respectively, or form large clusters at stoichiometric concentration of the protein. Daisy-like systems are formed under kinetic control conditions when nanoparticles first covered with the protein are treated with a second batch of biotinylated ones but devoid of avidin.
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spelling pubmed-82319602021-06-26 The Biotin–Avidin Interaction in Biotinylated Gold Nanoparticles and the Modulation of Their Aggregation Lyu, Yanchao Martínez, Álvaro D’Incà, Federica Mancin, Fabrizio Scrimin, Paolo Nanomaterials (Basel) Article The biotin–avidin interaction is used as a binding tool for the conjugation of biomolecules for more diverse applications; these include nanoparticle conjugation. Despite this, a thorough investigation on the different aggregates that may result from the interaction of biotinylated nanoparticles (gold nanoparticles, AuNPs, in this work) with avidin has not been carried out so far. In this paper, we address this problem and show the type of aggregates formed under thermodynamic and kinetic control by varying the biotinylated AuNP/avidin ratio and the order of addition of the two partners. The analysis was performed by also addressing the amount of protein able to interact with the AuNPs surface and is fully supported by the TEM images collected for the different samples and the shift of the surface plasmon resonance band. We show that the percentage of saturation depends on the size of the nanoparticles, and larger nanoparticles (19 nm in diameter) manage to accommodate a relatively larger amount of avidins than smaller ones (11 nm). The AuNPs are isolated or form small clusters (mostly dimers or trimers) when a large excess or a very low amount of avidin is present, respectively, or form large clusters at stoichiometric concentration of the protein. Daisy-like systems are formed under kinetic control conditions when nanoparticles first covered with the protein are treated with a second batch of biotinylated ones but devoid of avidin. MDPI 2021-06-13 /pmc/articles/PMC8231960/ /pubmed/34199307 http://dx.doi.org/10.3390/nano11061559 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lyu, Yanchao
Martínez, Álvaro
D’Incà, Federica
Mancin, Fabrizio
Scrimin, Paolo
The Biotin–Avidin Interaction in Biotinylated Gold Nanoparticles and the Modulation of Their Aggregation
title The Biotin–Avidin Interaction in Biotinylated Gold Nanoparticles and the Modulation of Their Aggregation
title_full The Biotin–Avidin Interaction in Biotinylated Gold Nanoparticles and the Modulation of Their Aggregation
title_fullStr The Biotin–Avidin Interaction in Biotinylated Gold Nanoparticles and the Modulation of Their Aggregation
title_full_unstemmed The Biotin–Avidin Interaction in Biotinylated Gold Nanoparticles and the Modulation of Their Aggregation
title_short The Biotin–Avidin Interaction in Biotinylated Gold Nanoparticles and the Modulation of Their Aggregation
title_sort biotin–avidin interaction in biotinylated gold nanoparticles and the modulation of their aggregation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8231960/
https://www.ncbi.nlm.nih.gov/pubmed/34199307
http://dx.doi.org/10.3390/nano11061559
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