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Proteomic Analysis of Fasciola hepatica Excretory and Secretory Products Co-Immunoprecipitated Using Time Course Infection Sera

Fasciola hepatica is a widespread pathogen that is known for its harmful effects on the health and productivity of ruminant animals. To identify the proteins present in all periods of infection with F. hepatica but not in those with Fasciola gigantica by shotgun liquid chromatography–tandem mass spe...

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Autores principales: Lan, Zhuo, Liu, Xiao-Lei, Lv, Qing-Bo, Zeng, Min-Hao, Gao, Jun-Feng, Chang, Qiao-Cheng, Chen, Yuan-Yuan, Wang, Chun-Ren
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8231988/
https://www.ncbi.nlm.nih.gov/pubmed/34199190
http://dx.doi.org/10.3390/pathogens10060749
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author Lan, Zhuo
Liu, Xiao-Lei
Lv, Qing-Bo
Zeng, Min-Hao
Gao, Jun-Feng
Chang, Qiao-Cheng
Chen, Yuan-Yuan
Wang, Chun-Ren
author_facet Lan, Zhuo
Liu, Xiao-Lei
Lv, Qing-Bo
Zeng, Min-Hao
Gao, Jun-Feng
Chang, Qiao-Cheng
Chen, Yuan-Yuan
Wang, Chun-Ren
author_sort Lan, Zhuo
collection PubMed
description Fasciola hepatica is a widespread pathogen that is known for its harmful effects on the health and productivity of ruminant animals. To identify the proteins present in all periods of infection with F. hepatica but not in those with Fasciola gigantica by shotgun liquid chromatography–tandem mass spectrometry (LC–MS/MS), we collected the ESPs and sera of F. hepatica and F. gigantica. In this study, the sheep were artificially infected with F. hepatica and the sera were collected at five different periods: 3 days post-infection (dpi), 7 dpi, 21 dpi, 63 dpi, and 112 dpi. The interacting proteins were pulled down from the sheep sera of all five periods and the sera with F. gigantica by co-immunoprecipitation (Co-IP) assay, before being identified by LC–MS/MS analysis. Thirty, twenty-two, twenty-three, twenty-seven, and twenty-two proteins were pulled down by the infected sera at 3 dpi, 7 dpi, 21 dpi, 63 dpi, and 112 dpi, respectively. Among them, 12 proteins existed in all periods, while six proteins could be detected in all periods in F. hepatica but not in F. gigantica. Protein relative pathway analysis revealed that these proteins mainly refer to the metabolism, regulation of genetic activity, and signal transduction of F. hepatica. In conclusion, this study provides meaningful data for the diagnosis of fasciolosis and to understand the interactions between F. hepatica and the host.
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spelling pubmed-82319882021-06-26 Proteomic Analysis of Fasciola hepatica Excretory and Secretory Products Co-Immunoprecipitated Using Time Course Infection Sera Lan, Zhuo Liu, Xiao-Lei Lv, Qing-Bo Zeng, Min-Hao Gao, Jun-Feng Chang, Qiao-Cheng Chen, Yuan-Yuan Wang, Chun-Ren Pathogens Article Fasciola hepatica is a widespread pathogen that is known for its harmful effects on the health and productivity of ruminant animals. To identify the proteins present in all periods of infection with F. hepatica but not in those with Fasciola gigantica by shotgun liquid chromatography–tandem mass spectrometry (LC–MS/MS), we collected the ESPs and sera of F. hepatica and F. gigantica. In this study, the sheep were artificially infected with F. hepatica and the sera were collected at five different periods: 3 days post-infection (dpi), 7 dpi, 21 dpi, 63 dpi, and 112 dpi. The interacting proteins were pulled down from the sheep sera of all five periods and the sera with F. gigantica by co-immunoprecipitation (Co-IP) assay, before being identified by LC–MS/MS analysis. Thirty, twenty-two, twenty-three, twenty-seven, and twenty-two proteins were pulled down by the infected sera at 3 dpi, 7 dpi, 21 dpi, 63 dpi, and 112 dpi, respectively. Among them, 12 proteins existed in all periods, while six proteins could be detected in all periods in F. hepatica but not in F. gigantica. Protein relative pathway analysis revealed that these proteins mainly refer to the metabolism, regulation of genetic activity, and signal transduction of F. hepatica. In conclusion, this study provides meaningful data for the diagnosis of fasciolosis and to understand the interactions between F. hepatica and the host. MDPI 2021-06-13 /pmc/articles/PMC8231988/ /pubmed/34199190 http://dx.doi.org/10.3390/pathogens10060749 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lan, Zhuo
Liu, Xiao-Lei
Lv, Qing-Bo
Zeng, Min-Hao
Gao, Jun-Feng
Chang, Qiao-Cheng
Chen, Yuan-Yuan
Wang, Chun-Ren
Proteomic Analysis of Fasciola hepatica Excretory and Secretory Products Co-Immunoprecipitated Using Time Course Infection Sera
title Proteomic Analysis of Fasciola hepatica Excretory and Secretory Products Co-Immunoprecipitated Using Time Course Infection Sera
title_full Proteomic Analysis of Fasciola hepatica Excretory and Secretory Products Co-Immunoprecipitated Using Time Course Infection Sera
title_fullStr Proteomic Analysis of Fasciola hepatica Excretory and Secretory Products Co-Immunoprecipitated Using Time Course Infection Sera
title_full_unstemmed Proteomic Analysis of Fasciola hepatica Excretory and Secretory Products Co-Immunoprecipitated Using Time Course Infection Sera
title_short Proteomic Analysis of Fasciola hepatica Excretory and Secretory Products Co-Immunoprecipitated Using Time Course Infection Sera
title_sort proteomic analysis of fasciola hepatica excretory and secretory products co-immunoprecipitated using time course infection sera
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8231988/
https://www.ncbi.nlm.nih.gov/pubmed/34199190
http://dx.doi.org/10.3390/pathogens10060749
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