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Molecular and Functional Evolution of the Spermatophyte Sesquiterpene Synthases

Sesquiterpenes are important defense and signal molecules for plants to adapt to the environment, cope with stress, and communicate with the outside world, and their evolutionary history is closely related to physiological functions. In this study, the information of plant sesquiterpene synthases (S...

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Autores principales: Liang, Dongmei, Li, Weiguo, Yan, Xiaoguang, Caiyin, Qinggele, Zhao, Guangrong, Qiao, Jianjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8232007/
https://www.ncbi.nlm.nih.gov/pubmed/34198531
http://dx.doi.org/10.3390/ijms22126348
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author Liang, Dongmei
Li, Weiguo
Yan, Xiaoguang
Caiyin, Qinggele
Zhao, Guangrong
Qiao, Jianjun
author_facet Liang, Dongmei
Li, Weiguo
Yan, Xiaoguang
Caiyin, Qinggele
Zhao, Guangrong
Qiao, Jianjun
author_sort Liang, Dongmei
collection PubMed
description Sesquiterpenes are important defense and signal molecules for plants to adapt to the environment, cope with stress, and communicate with the outside world, and their evolutionary history is closely related to physiological functions. In this study, the information of plant sesquiterpene synthases (STSs) with identified functions were collected and sorted to form a dataset containing about 500 members. The phylogeny of spermatophyte functional STSs was constructed based on the structural comparative analysis to reveal the sequence–structure–function relationships. We propose the evolutionary history of plant sesquiterpene skeletons, from chain structure to small rings, followed by large rings for the first time and put forward a more detailed function-driven hypothesis. Then, the evolutionary origins and history of spermatophyte STSs are also discussed. In addition, three newly identified STSs CaSTS2, CaSTS3, and CaSTS4 were analyzed in this functional evolutionary system, and their germacrene D products were consistent with the functional prediction. This demonstrates an application of the structure-based phylogeny in predicting STS function. This work will help us to understand evolutionary patterns and dynamics of plant sesquiterpenes and STSs and screen or design STSs with specific product profiles as functional elements for synthetic biology application.
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spelling pubmed-82320072021-06-26 Molecular and Functional Evolution of the Spermatophyte Sesquiterpene Synthases Liang, Dongmei Li, Weiguo Yan, Xiaoguang Caiyin, Qinggele Zhao, Guangrong Qiao, Jianjun Int J Mol Sci Article Sesquiterpenes are important defense and signal molecules for plants to adapt to the environment, cope with stress, and communicate with the outside world, and their evolutionary history is closely related to physiological functions. In this study, the information of plant sesquiterpene synthases (STSs) with identified functions were collected and sorted to form a dataset containing about 500 members. The phylogeny of spermatophyte functional STSs was constructed based on the structural comparative analysis to reveal the sequence–structure–function relationships. We propose the evolutionary history of plant sesquiterpene skeletons, from chain structure to small rings, followed by large rings for the first time and put forward a more detailed function-driven hypothesis. Then, the evolutionary origins and history of spermatophyte STSs are also discussed. In addition, three newly identified STSs CaSTS2, CaSTS3, and CaSTS4 were analyzed in this functional evolutionary system, and their germacrene D products were consistent with the functional prediction. This demonstrates an application of the structure-based phylogeny in predicting STS function. This work will help us to understand evolutionary patterns and dynamics of plant sesquiterpenes and STSs and screen or design STSs with specific product profiles as functional elements for synthetic biology application. MDPI 2021-06-14 /pmc/articles/PMC8232007/ /pubmed/34198531 http://dx.doi.org/10.3390/ijms22126348 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liang, Dongmei
Li, Weiguo
Yan, Xiaoguang
Caiyin, Qinggele
Zhao, Guangrong
Qiao, Jianjun
Molecular and Functional Evolution of the Spermatophyte Sesquiterpene Synthases
title Molecular and Functional Evolution of the Spermatophyte Sesquiterpene Synthases
title_full Molecular and Functional Evolution of the Spermatophyte Sesquiterpene Synthases
title_fullStr Molecular and Functional Evolution of the Spermatophyte Sesquiterpene Synthases
title_full_unstemmed Molecular and Functional Evolution of the Spermatophyte Sesquiterpene Synthases
title_short Molecular and Functional Evolution of the Spermatophyte Sesquiterpene Synthases
title_sort molecular and functional evolution of the spermatophyte sesquiterpene synthases
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8232007/
https://www.ncbi.nlm.nih.gov/pubmed/34198531
http://dx.doi.org/10.3390/ijms22126348
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