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A Study on the Antimicrobial and Antibiofilm Peptide 1018-K6 as Potential Alternative to Antibiotics against Food-Pathogen Salmonella enterica

Antimicrobial resistance has become one of the major global public health concerns, and it is indispensable to search for alternatives to conventional antibiotics. Recently, antimicrobial peptides have received great attention because of their broad-spectrum antimicrobial activity at relatively low...

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Autores principales: Festa, Rossella, Ambrosio, Rosa Luisa, Lamas, Alexandre, Gratino, Lorena, Palmieri, Gianna, Franco, Carlos Manuel, Cepeda, Alberto, Anastasio, Aniello
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8232012/
https://www.ncbi.nlm.nih.gov/pubmed/34198540
http://dx.doi.org/10.3390/foods10061372
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author Festa, Rossella
Ambrosio, Rosa Luisa
Lamas, Alexandre
Gratino, Lorena
Palmieri, Gianna
Franco, Carlos Manuel
Cepeda, Alberto
Anastasio, Aniello
author_facet Festa, Rossella
Ambrosio, Rosa Luisa
Lamas, Alexandre
Gratino, Lorena
Palmieri, Gianna
Franco, Carlos Manuel
Cepeda, Alberto
Anastasio, Aniello
author_sort Festa, Rossella
collection PubMed
description Antimicrobial resistance has become one of the major global public health concerns, and it is indispensable to search for alternatives to conventional antibiotics. Recently, antimicrobial peptides have received great attention because of their broad-spectrum antimicrobial activity at relatively low concentrations, even against pathogens such as Salmonella enterica, which is responsible for most food-borne illnesses. This work aimed at evaluating the antimicrobial and antibiofilm activity of the innate defense peptide, named 1018-K6, against S. enterica. A total of 42 strains, belonging to three different subspecies and 32 serotypes, were included in this study. The antibiotic resistance profile of all the strains and the cytotoxic effects of 1018-K6 on mammalian fibroblast cells were also investigated. Results revealed that MIC (minimum inhibitory concentrations) and MBC (minimum bactericidal concentrations) values were in the ranges of 8–64 μg/mL and 16–128 μg/mL, respectively, although most strains (97%) showed MICs between 16 and 32 μg/mL. Moreover, sub-inhibitory concentrations of 1018-K6 strongly reduced the biofilm formation in several S. enterica strains, whatever the initial inoculum size. Our results demonstrated that 1018-K6 is able to control and manage S. enterica growth with a large potential for applications in the fields of active packaging and water disinfectants.
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spelling pubmed-82320122021-06-26 A Study on the Antimicrobial and Antibiofilm Peptide 1018-K6 as Potential Alternative to Antibiotics against Food-Pathogen Salmonella enterica Festa, Rossella Ambrosio, Rosa Luisa Lamas, Alexandre Gratino, Lorena Palmieri, Gianna Franco, Carlos Manuel Cepeda, Alberto Anastasio, Aniello Foods Article Antimicrobial resistance has become one of the major global public health concerns, and it is indispensable to search for alternatives to conventional antibiotics. Recently, antimicrobial peptides have received great attention because of their broad-spectrum antimicrobial activity at relatively low concentrations, even against pathogens such as Salmonella enterica, which is responsible for most food-borne illnesses. This work aimed at evaluating the antimicrobial and antibiofilm activity of the innate defense peptide, named 1018-K6, against S. enterica. A total of 42 strains, belonging to three different subspecies and 32 serotypes, were included in this study. The antibiotic resistance profile of all the strains and the cytotoxic effects of 1018-K6 on mammalian fibroblast cells were also investigated. Results revealed that MIC (minimum inhibitory concentrations) and MBC (minimum bactericidal concentrations) values were in the ranges of 8–64 μg/mL and 16–128 μg/mL, respectively, although most strains (97%) showed MICs between 16 and 32 μg/mL. Moreover, sub-inhibitory concentrations of 1018-K6 strongly reduced the biofilm formation in several S. enterica strains, whatever the initial inoculum size. Our results demonstrated that 1018-K6 is able to control and manage S. enterica growth with a large potential for applications in the fields of active packaging and water disinfectants. MDPI 2021-06-14 /pmc/articles/PMC8232012/ /pubmed/34198540 http://dx.doi.org/10.3390/foods10061372 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Festa, Rossella
Ambrosio, Rosa Luisa
Lamas, Alexandre
Gratino, Lorena
Palmieri, Gianna
Franco, Carlos Manuel
Cepeda, Alberto
Anastasio, Aniello
A Study on the Antimicrobial and Antibiofilm Peptide 1018-K6 as Potential Alternative to Antibiotics against Food-Pathogen Salmonella enterica
title A Study on the Antimicrobial and Antibiofilm Peptide 1018-K6 as Potential Alternative to Antibiotics against Food-Pathogen Salmonella enterica
title_full A Study on the Antimicrobial and Antibiofilm Peptide 1018-K6 as Potential Alternative to Antibiotics against Food-Pathogen Salmonella enterica
title_fullStr A Study on the Antimicrobial and Antibiofilm Peptide 1018-K6 as Potential Alternative to Antibiotics against Food-Pathogen Salmonella enterica
title_full_unstemmed A Study on the Antimicrobial and Antibiofilm Peptide 1018-K6 as Potential Alternative to Antibiotics against Food-Pathogen Salmonella enterica
title_short A Study on the Antimicrobial and Antibiofilm Peptide 1018-K6 as Potential Alternative to Antibiotics against Food-Pathogen Salmonella enterica
title_sort study on the antimicrobial and antibiofilm peptide 1018-k6 as potential alternative to antibiotics against food-pathogen salmonella enterica
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8232012/
https://www.ncbi.nlm.nih.gov/pubmed/34198540
http://dx.doi.org/10.3390/foods10061372
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