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Knockout of a highly GC‐rich gene in Burkholderia pyrrocinia by recombineering with freeze‐thawing transformation

Genetic transformation is a valuable and essential method that provides powerful insights into the gene function of microorganisms and contributes to the construction of engineered bacteria. Here, we developed a novel genetic transformation system to easily knock out a highly GC‐rich gene (74.71% GC...

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Detalles Bibliográficos
Autores principales:	Chen, Feifei, Ye, Jianren, Liu, Wanhui, Chio, Chonlong, Wang, Wendy, Qin, Wensheng
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	John Wiley and Sons Inc. 2021
Materias:	Technical Advance
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8232026/ https://www.ncbi.nlm.nih.gov/pubmed/33942460 http://dx.doi.org/10.1111/mpp.13058

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