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Development of a Diagnostic Biosensor Method of Hypersensitivity Pneumonitis towards a Point-of-Care Biosensor
In spite of a current increasing trend in the development of miniaturized, standalone point-of-care (PoC) biosensing platforms in the literature, the actual implementation of such systems in the field is far from being a reality although deeply needed. In the particular case of the population screen...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8232117/ https://www.ncbi.nlm.nih.gov/pubmed/34203685 http://dx.doi.org/10.3390/bios11060196 |
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author | Fiordelisio, Tatiana Buendia-Roldan, Ivette Hautefeuille, Mathieu Del-Rio, Diana Ríos-López, Diana G. Zamarrón-Hernández, Diego Amat-Shapiro, Samuel Campa-Higareda, Andrea Jiménez-Díaz, Edgar González-Villa, Erika Nelson-Mora, Janikua García-Carreño, Natllely López-Aparicio, Jehú Montes, Eduardo Santiago-Ruiz, Armando Pardo, Annie Selman, Moisés |
author_facet | Fiordelisio, Tatiana Buendia-Roldan, Ivette Hautefeuille, Mathieu Del-Rio, Diana Ríos-López, Diana G. Zamarrón-Hernández, Diego Amat-Shapiro, Samuel Campa-Higareda, Andrea Jiménez-Díaz, Edgar González-Villa, Erika Nelson-Mora, Janikua García-Carreño, Natllely López-Aparicio, Jehú Montes, Eduardo Santiago-Ruiz, Armando Pardo, Annie Selman, Moisés |
author_sort | Fiordelisio, Tatiana |
collection | PubMed |
description | In spite of a current increasing trend in the development of miniaturized, standalone point-of-care (PoC) biosensing platforms in the literature, the actual implementation of such systems in the field is far from being a reality although deeply needed. In the particular case of the population screenings for local or regional diseases related to specific pathogens, the diagnosis of the presence of specific antibodies could drastically modify therapies and even the organization of public policies. The aim of this work was to develop a fast, cost-effective detection method based on the manipulation of functionalized magnetic beads for an efficient diagnosis of hypersensitivity pneumonitis (HP), looking for the presence of anti-pigeon antigen antibodies (APAA) in a patient’s serum. We presented a Diagnostic Biosensor Method (DBM) in detail, with validation by comparison with a traditional high-throughput platform (ELISA assay). We also demonstrated that it was compatible with a microfluidic chip that could be eventually incorporated into a PoC for easy and broad deployment using portable optical detectors. After standardization of the different reaction steps, we constructed and validated a plastic chip that could easily be scaled to high-volume manufacturing in the future. The solution proved comparable to conventional ELISA assays traditionally performed by the clinicians in their laboratory and should be compatible with other antibody detection directly from patient samples. |
format | Online Article Text |
id | pubmed-8232117 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-82321172021-06-26 Development of a Diagnostic Biosensor Method of Hypersensitivity Pneumonitis towards a Point-of-Care Biosensor Fiordelisio, Tatiana Buendia-Roldan, Ivette Hautefeuille, Mathieu Del-Rio, Diana Ríos-López, Diana G. Zamarrón-Hernández, Diego Amat-Shapiro, Samuel Campa-Higareda, Andrea Jiménez-Díaz, Edgar González-Villa, Erika Nelson-Mora, Janikua García-Carreño, Natllely López-Aparicio, Jehú Montes, Eduardo Santiago-Ruiz, Armando Pardo, Annie Selman, Moisés Biosensors (Basel) Article In spite of a current increasing trend in the development of miniaturized, standalone point-of-care (PoC) biosensing platforms in the literature, the actual implementation of such systems in the field is far from being a reality although deeply needed. In the particular case of the population screenings for local or regional diseases related to specific pathogens, the diagnosis of the presence of specific antibodies could drastically modify therapies and even the organization of public policies. The aim of this work was to develop a fast, cost-effective detection method based on the manipulation of functionalized magnetic beads for an efficient diagnosis of hypersensitivity pneumonitis (HP), looking for the presence of anti-pigeon antigen antibodies (APAA) in a patient’s serum. We presented a Diagnostic Biosensor Method (DBM) in detail, with validation by comparison with a traditional high-throughput platform (ELISA assay). We also demonstrated that it was compatible with a microfluidic chip that could be eventually incorporated into a PoC for easy and broad deployment using portable optical detectors. After standardization of the different reaction steps, we constructed and validated a plastic chip that could easily be scaled to high-volume manufacturing in the future. The solution proved comparable to conventional ELISA assays traditionally performed by the clinicians in their laboratory and should be compatible with other antibody detection directly from patient samples. MDPI 2021-06-15 /pmc/articles/PMC8232117/ /pubmed/34203685 http://dx.doi.org/10.3390/bios11060196 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Fiordelisio, Tatiana Buendia-Roldan, Ivette Hautefeuille, Mathieu Del-Rio, Diana Ríos-López, Diana G. Zamarrón-Hernández, Diego Amat-Shapiro, Samuel Campa-Higareda, Andrea Jiménez-Díaz, Edgar González-Villa, Erika Nelson-Mora, Janikua García-Carreño, Natllely López-Aparicio, Jehú Montes, Eduardo Santiago-Ruiz, Armando Pardo, Annie Selman, Moisés Development of a Diagnostic Biosensor Method of Hypersensitivity Pneumonitis towards a Point-of-Care Biosensor |
title | Development of a Diagnostic Biosensor Method of Hypersensitivity Pneumonitis towards a Point-of-Care Biosensor |
title_full | Development of a Diagnostic Biosensor Method of Hypersensitivity Pneumonitis towards a Point-of-Care Biosensor |
title_fullStr | Development of a Diagnostic Biosensor Method of Hypersensitivity Pneumonitis towards a Point-of-Care Biosensor |
title_full_unstemmed | Development of a Diagnostic Biosensor Method of Hypersensitivity Pneumonitis towards a Point-of-Care Biosensor |
title_short | Development of a Diagnostic Biosensor Method of Hypersensitivity Pneumonitis towards a Point-of-Care Biosensor |
title_sort | development of a diagnostic biosensor method of hypersensitivity pneumonitis towards a point-of-care biosensor |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8232117/ https://www.ncbi.nlm.nih.gov/pubmed/34203685 http://dx.doi.org/10.3390/bios11060196 |
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