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Transcriptome-based identification and expression characterization of RgABCC transporters in Rehmannia glutinosa
ABCC multidrug resistance-associated proteins (ABCCs/MRPs), a subfamily of ABC transporters, are involved in multiple physiological processes. Although these proteins have been characterized in some plants, limited efforts have been made to address their possible roles in Rehmannia glutinosa, a medi...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8232422/ https://www.ncbi.nlm.nih.gov/pubmed/34170906 http://dx.doi.org/10.1371/journal.pone.0253188 |
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author | Yang, Yan Hui Wang, Chao Jie Li, Rui Fang Yi, Yan Jie Zeng, Lei Yang, Heng Zhang, Chang Fu Song, Kai Yi Guo, Si Jiao |
author_facet | Yang, Yan Hui Wang, Chao Jie Li, Rui Fang Yi, Yan Jie Zeng, Lei Yang, Heng Zhang, Chang Fu Song, Kai Yi Guo, Si Jiao |
author_sort | Yang, Yan Hui |
collection | PubMed |
description | ABCC multidrug resistance-associated proteins (ABCCs/MRPs), a subfamily of ABC transporters, are involved in multiple physiological processes. Although these proteins have been characterized in some plants, limited efforts have been made to address their possible roles in Rehmannia glutinosa, a medicinal plant. Here, we scanned R. glutinosa transcriptome sequences and identified 18 RgABCC genes by in silico analysis. Sequence alignment revealed that the RgABCCs were closely phylogenetically related and highly conserved with other plant ABCCs/MRPs. Subcellular localization revealed that most of the RgABCCs were deposited in vacuoles and a few in plasma membranes. Tissue-specific expression of the RgABCCs indicated significant specific accumulation patterns, implicating their roles in the respective tissues. Differential temporal expression patterns of the RgABCCs exhibited their potential roles during root development. Various abiotic stress and hormone treatment experiments indicated that some RgABCCs could be transcriptionally regulated in roots. Furthermore, the transcription of several RgABCCs in roots was strongly activated by cadmium (Cd), suggesting possible roles under heavy metal stresses. Functional analysis of RgABCC1 heterologous expression revealed that it may increase the tolerance to Cd in yeast, implying its Cd transport activity. Our study provides a detailed inventory and molecular characterization of the RgABCCs and valuable information for exploring their functions in R. glutinosa. |
format | Online Article Text |
id | pubmed-8232422 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-82324222021-07-07 Transcriptome-based identification and expression characterization of RgABCC transporters in Rehmannia glutinosa Yang, Yan Hui Wang, Chao Jie Li, Rui Fang Yi, Yan Jie Zeng, Lei Yang, Heng Zhang, Chang Fu Song, Kai Yi Guo, Si Jiao PLoS One Research Article ABCC multidrug resistance-associated proteins (ABCCs/MRPs), a subfamily of ABC transporters, are involved in multiple physiological processes. Although these proteins have been characterized in some plants, limited efforts have been made to address their possible roles in Rehmannia glutinosa, a medicinal plant. Here, we scanned R. glutinosa transcriptome sequences and identified 18 RgABCC genes by in silico analysis. Sequence alignment revealed that the RgABCCs were closely phylogenetically related and highly conserved with other plant ABCCs/MRPs. Subcellular localization revealed that most of the RgABCCs were deposited in vacuoles and a few in plasma membranes. Tissue-specific expression of the RgABCCs indicated significant specific accumulation patterns, implicating their roles in the respective tissues. Differential temporal expression patterns of the RgABCCs exhibited their potential roles during root development. Various abiotic stress and hormone treatment experiments indicated that some RgABCCs could be transcriptionally regulated in roots. Furthermore, the transcription of several RgABCCs in roots was strongly activated by cadmium (Cd), suggesting possible roles under heavy metal stresses. Functional analysis of RgABCC1 heterologous expression revealed that it may increase the tolerance to Cd in yeast, implying its Cd transport activity. Our study provides a detailed inventory and molecular characterization of the RgABCCs and valuable information for exploring their functions in R. glutinosa. Public Library of Science 2021-06-25 /pmc/articles/PMC8232422/ /pubmed/34170906 http://dx.doi.org/10.1371/journal.pone.0253188 Text en © 2021 Yang et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Yang, Yan Hui Wang, Chao Jie Li, Rui Fang Yi, Yan Jie Zeng, Lei Yang, Heng Zhang, Chang Fu Song, Kai Yi Guo, Si Jiao Transcriptome-based identification and expression characterization of RgABCC transporters in Rehmannia glutinosa |
title | Transcriptome-based identification and expression characterization of RgABCC transporters in Rehmannia glutinosa |
title_full | Transcriptome-based identification and expression characterization of RgABCC transporters in Rehmannia glutinosa |
title_fullStr | Transcriptome-based identification and expression characterization of RgABCC transporters in Rehmannia glutinosa |
title_full_unstemmed | Transcriptome-based identification and expression characterization of RgABCC transporters in Rehmannia glutinosa |
title_short | Transcriptome-based identification and expression characterization of RgABCC transporters in Rehmannia glutinosa |
title_sort | transcriptome-based identification and expression characterization of rgabcc transporters in rehmannia glutinosa |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8232422/ https://www.ncbi.nlm.nih.gov/pubmed/34170906 http://dx.doi.org/10.1371/journal.pone.0253188 |
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