Cargando…
Could serum zonulin be an intestinal permeability marker in diabetes kidney disease?
Zonulin is a protein associated with the tight junction complex opening at the intestinal epithelium, previously linked to obesity, cardiovascular diseases, type 2 diabetes mellitus (T2DM) and chronic kidney disease (CKD). However, its role in CKD has not been totally elucidated. This study aimed to...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8232531/ https://www.ncbi.nlm.nih.gov/pubmed/34170951 http://dx.doi.org/10.1371/journal.pone.0253501 |
_version_ | 1783713657295732736 |
---|---|
author | Carpes, Larissa Salomoni Nicoletto, Bruna Bellincanta Canani, Luis Henrique Rheinhemer, Jakeline Crispim, Daisy Souza, Gabriela Corrêa |
author_facet | Carpes, Larissa Salomoni Nicoletto, Bruna Bellincanta Canani, Luis Henrique Rheinhemer, Jakeline Crispim, Daisy Souza, Gabriela Corrêa |
author_sort | Carpes, Larissa Salomoni |
collection | PubMed |
description | Zonulin is a protein associated with the tight junction complex opening at the intestinal epithelium, previously linked to obesity, cardiovascular diseases, type 2 diabetes mellitus (T2DM) and chronic kidney disease (CKD). However, its role in CKD has not been totally elucidated. This study aimed to evaluate zonulin levels in subjects with diabetic kidney disease (DKD). This case-control study included two cases groups: 1) Advanced DKD cases: T2DM patients with estimated glomerular filtration rate (eGFR) <60ml/min/1.73m(2); 2) Albuminuric T2DM cases: diabetic patients with urinary albumin excretion (UAE) >30mg/g creatinine, but with eGFR>60ml/min/1.73m(2). Two control groups were also included: 1) T2DM controls: patients with T2DM without impaired kidney function; 2) Non-T2DM controls: subjects without T2DM and normal renal function. Serum levels of zonulin were measured by ELISA. Eighty-six individuals were included. Zonulin levels was different among study groups (P = 0.003). T2DM controls presented higher zonulin levels than non-T2DM controls [(131.35 (83.0–170.5) vs. 87.25 (54.7–111.8), P = 0.018] and advanced DKD cases [63.72 (45.03–106.0); P = 0.007]. Zonulin showed a positive correlation with eGFR (r = 0.222; P = 0.040), total cholesterol (r = 0.299; P = 0.034), LDL (r = 0.258; P = 0.021), and negative with albuminuria (r = -0.243; P = 0.024) and body fat (r = -0.271; P = 0.014). In the multivariate logistic regression analyses, zonulin levels were independently associated to renal outcomes [OR 0.99 (0.98–0.99, P = 0.012)] after 5-year inclusion. In conclusion, increased zonulin levels in patients with TD2M without renal disease suggest an impaired intestinal permeability. Moreover, its association with renal outcomes could indicate its use as a disease monitoring marker. However, the mechanisms behind this association should be better understood. |
format | Online Article Text |
id | pubmed-8232531 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-82325312021-07-07 Could serum zonulin be an intestinal permeability marker in diabetes kidney disease? Carpes, Larissa Salomoni Nicoletto, Bruna Bellincanta Canani, Luis Henrique Rheinhemer, Jakeline Crispim, Daisy Souza, Gabriela Corrêa PLoS One Research Article Zonulin is a protein associated with the tight junction complex opening at the intestinal epithelium, previously linked to obesity, cardiovascular diseases, type 2 diabetes mellitus (T2DM) and chronic kidney disease (CKD). However, its role in CKD has not been totally elucidated. This study aimed to evaluate zonulin levels in subjects with diabetic kidney disease (DKD). This case-control study included two cases groups: 1) Advanced DKD cases: T2DM patients with estimated glomerular filtration rate (eGFR) <60ml/min/1.73m(2); 2) Albuminuric T2DM cases: diabetic patients with urinary albumin excretion (UAE) >30mg/g creatinine, but with eGFR>60ml/min/1.73m(2). Two control groups were also included: 1) T2DM controls: patients with T2DM without impaired kidney function; 2) Non-T2DM controls: subjects without T2DM and normal renal function. Serum levels of zonulin were measured by ELISA. Eighty-six individuals were included. Zonulin levels was different among study groups (P = 0.003). T2DM controls presented higher zonulin levels than non-T2DM controls [(131.35 (83.0–170.5) vs. 87.25 (54.7–111.8), P = 0.018] and advanced DKD cases [63.72 (45.03–106.0); P = 0.007]. Zonulin showed a positive correlation with eGFR (r = 0.222; P = 0.040), total cholesterol (r = 0.299; P = 0.034), LDL (r = 0.258; P = 0.021), and negative with albuminuria (r = -0.243; P = 0.024) and body fat (r = -0.271; P = 0.014). In the multivariate logistic regression analyses, zonulin levels were independently associated to renal outcomes [OR 0.99 (0.98–0.99, P = 0.012)] after 5-year inclusion. In conclusion, increased zonulin levels in patients with TD2M without renal disease suggest an impaired intestinal permeability. Moreover, its association with renal outcomes could indicate its use as a disease monitoring marker. However, the mechanisms behind this association should be better understood. Public Library of Science 2021-06-25 /pmc/articles/PMC8232531/ /pubmed/34170951 http://dx.doi.org/10.1371/journal.pone.0253501 Text en © 2021 Carpes et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Carpes, Larissa Salomoni Nicoletto, Bruna Bellincanta Canani, Luis Henrique Rheinhemer, Jakeline Crispim, Daisy Souza, Gabriela Corrêa Could serum zonulin be an intestinal permeability marker in diabetes kidney disease? |
title | Could serum zonulin be an intestinal permeability marker in diabetes kidney disease? |
title_full | Could serum zonulin be an intestinal permeability marker in diabetes kidney disease? |
title_fullStr | Could serum zonulin be an intestinal permeability marker in diabetes kidney disease? |
title_full_unstemmed | Could serum zonulin be an intestinal permeability marker in diabetes kidney disease? |
title_short | Could serum zonulin be an intestinal permeability marker in diabetes kidney disease? |
title_sort | could serum zonulin be an intestinal permeability marker in diabetes kidney disease? |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8232531/ https://www.ncbi.nlm.nih.gov/pubmed/34170951 http://dx.doi.org/10.1371/journal.pone.0253501 |
work_keys_str_mv | AT carpeslarissasalomoni couldserumzonulinbeanintestinalpermeabilitymarkerindiabeteskidneydisease AT nicolettobrunabellincanta couldserumzonulinbeanintestinalpermeabilitymarkerindiabeteskidneydisease AT cananiluishenrique couldserumzonulinbeanintestinalpermeabilitymarkerindiabeteskidneydisease AT rheinhemerjakeline couldserumzonulinbeanintestinalpermeabilitymarkerindiabeteskidneydisease AT crispimdaisy couldserumzonulinbeanintestinalpermeabilitymarkerindiabeteskidneydisease AT souzagabrielacorrea couldserumzonulinbeanintestinalpermeabilitymarkerindiabeteskidneydisease |