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Could serum zonulin be an intestinal permeability marker in diabetes kidney disease?

Zonulin is a protein associated with the tight junction complex opening at the intestinal epithelium, previously linked to obesity, cardiovascular diseases, type 2 diabetes mellitus (T2DM) and chronic kidney disease (CKD). However, its role in CKD has not been totally elucidated. This study aimed to...

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Autores principales: Carpes, Larissa Salomoni, Nicoletto, Bruna Bellincanta, Canani, Luis Henrique, Rheinhemer, Jakeline, Crispim, Daisy, Souza, Gabriela Corrêa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8232531/
https://www.ncbi.nlm.nih.gov/pubmed/34170951
http://dx.doi.org/10.1371/journal.pone.0253501
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author Carpes, Larissa Salomoni
Nicoletto, Bruna Bellincanta
Canani, Luis Henrique
Rheinhemer, Jakeline
Crispim, Daisy
Souza, Gabriela Corrêa
author_facet Carpes, Larissa Salomoni
Nicoletto, Bruna Bellincanta
Canani, Luis Henrique
Rheinhemer, Jakeline
Crispim, Daisy
Souza, Gabriela Corrêa
author_sort Carpes, Larissa Salomoni
collection PubMed
description Zonulin is a protein associated with the tight junction complex opening at the intestinal epithelium, previously linked to obesity, cardiovascular diseases, type 2 diabetes mellitus (T2DM) and chronic kidney disease (CKD). However, its role in CKD has not been totally elucidated. This study aimed to evaluate zonulin levels in subjects with diabetic kidney disease (DKD). This case-control study included two cases groups: 1) Advanced DKD cases: T2DM patients with estimated glomerular filtration rate (eGFR) <60ml/min/1.73m(2); 2) Albuminuric T2DM cases: diabetic patients with urinary albumin excretion (UAE) >30mg/g creatinine, but with eGFR>60ml/min/1.73m(2). Two control groups were also included: 1) T2DM controls: patients with T2DM without impaired kidney function; 2) Non-T2DM controls: subjects without T2DM and normal renal function. Serum levels of zonulin were measured by ELISA. Eighty-six individuals were included. Zonulin levels was different among study groups (P = 0.003). T2DM controls presented higher zonulin levels than non-T2DM controls [(131.35 (83.0–170.5) vs. 87.25 (54.7–111.8), P = 0.018] and advanced DKD cases [63.72 (45.03–106.0); P = 0.007]. Zonulin showed a positive correlation with eGFR (r = 0.222; P = 0.040), total cholesterol (r = 0.299; P = 0.034), LDL (r = 0.258; P = 0.021), and negative with albuminuria (r = -0.243; P = 0.024) and body fat (r = -0.271; P = 0.014). In the multivariate logistic regression analyses, zonulin levels were independently associated to renal outcomes [OR 0.99 (0.98–0.99, P = 0.012)] after 5-year inclusion. In conclusion, increased zonulin levels in patients with TD2M without renal disease suggest an impaired intestinal permeability. Moreover, its association with renal outcomes could indicate its use as a disease monitoring marker. However, the mechanisms behind this association should be better understood.
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spelling pubmed-82325312021-07-07 Could serum zonulin be an intestinal permeability marker in diabetes kidney disease? Carpes, Larissa Salomoni Nicoletto, Bruna Bellincanta Canani, Luis Henrique Rheinhemer, Jakeline Crispim, Daisy Souza, Gabriela Corrêa PLoS One Research Article Zonulin is a protein associated with the tight junction complex opening at the intestinal epithelium, previously linked to obesity, cardiovascular diseases, type 2 diabetes mellitus (T2DM) and chronic kidney disease (CKD). However, its role in CKD has not been totally elucidated. This study aimed to evaluate zonulin levels in subjects with diabetic kidney disease (DKD). This case-control study included two cases groups: 1) Advanced DKD cases: T2DM patients with estimated glomerular filtration rate (eGFR) <60ml/min/1.73m(2); 2) Albuminuric T2DM cases: diabetic patients with urinary albumin excretion (UAE) >30mg/g creatinine, but with eGFR>60ml/min/1.73m(2). Two control groups were also included: 1) T2DM controls: patients with T2DM without impaired kidney function; 2) Non-T2DM controls: subjects without T2DM and normal renal function. Serum levels of zonulin were measured by ELISA. Eighty-six individuals were included. Zonulin levels was different among study groups (P = 0.003). T2DM controls presented higher zonulin levels than non-T2DM controls [(131.35 (83.0–170.5) vs. 87.25 (54.7–111.8), P = 0.018] and advanced DKD cases [63.72 (45.03–106.0); P = 0.007]. Zonulin showed a positive correlation with eGFR (r = 0.222; P = 0.040), total cholesterol (r = 0.299; P = 0.034), LDL (r = 0.258; P = 0.021), and negative with albuminuria (r = -0.243; P = 0.024) and body fat (r = -0.271; P = 0.014). In the multivariate logistic regression analyses, zonulin levels were independently associated to renal outcomes [OR 0.99 (0.98–0.99, P = 0.012)] after 5-year inclusion. In conclusion, increased zonulin levels in patients with TD2M without renal disease suggest an impaired intestinal permeability. Moreover, its association with renal outcomes could indicate its use as a disease monitoring marker. However, the mechanisms behind this association should be better understood. Public Library of Science 2021-06-25 /pmc/articles/PMC8232531/ /pubmed/34170951 http://dx.doi.org/10.1371/journal.pone.0253501 Text en © 2021 Carpes et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Carpes, Larissa Salomoni
Nicoletto, Bruna Bellincanta
Canani, Luis Henrique
Rheinhemer, Jakeline
Crispim, Daisy
Souza, Gabriela Corrêa
Could serum zonulin be an intestinal permeability marker in diabetes kidney disease?
title Could serum zonulin be an intestinal permeability marker in diabetes kidney disease?
title_full Could serum zonulin be an intestinal permeability marker in diabetes kidney disease?
title_fullStr Could serum zonulin be an intestinal permeability marker in diabetes kidney disease?
title_full_unstemmed Could serum zonulin be an intestinal permeability marker in diabetes kidney disease?
title_short Could serum zonulin be an intestinal permeability marker in diabetes kidney disease?
title_sort could serum zonulin be an intestinal permeability marker in diabetes kidney disease?
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8232531/
https://www.ncbi.nlm.nih.gov/pubmed/34170951
http://dx.doi.org/10.1371/journal.pone.0253501
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