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Proteomics of Homeobox7 Enhanced Salt Tolerance in Mesembryanthemum crystallinum

Mesembryanthemum crystallinum (common ice plant) is a halophyte species that has adapted to extreme conditions. In this study, we cloned a McHB7 transcription factor gene from the ice plant. The expression of McHB7 was significantly induced by 500 mM NaCl and it reached the peak under salt treatment...

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Autores principales: Zhang, Xuemei, Tan, Bowen, Zhu, Dan, Dufresne, Daniel, Jiang, Tingbo, Chen, Sixue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8232686/
https://www.ncbi.nlm.nih.gov/pubmed/34203768
http://dx.doi.org/10.3390/ijms22126390
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author Zhang, Xuemei
Tan, Bowen
Zhu, Dan
Dufresne, Daniel
Jiang, Tingbo
Chen, Sixue
author_facet Zhang, Xuemei
Tan, Bowen
Zhu, Dan
Dufresne, Daniel
Jiang, Tingbo
Chen, Sixue
author_sort Zhang, Xuemei
collection PubMed
description Mesembryanthemum crystallinum (common ice plant) is a halophyte species that has adapted to extreme conditions. In this study, we cloned a McHB7 transcription factor gene from the ice plant. The expression of McHB7 was significantly induced by 500 mM NaCl and it reached the peak under salt treatment for 7 days. The McHB7 protein was targeted to the nucleus. McHB7-overexpressing in ice plant leaves through Agrobacterium-mediated transformation led to 25 times more McHB7 transcripts than the non-transformed wild type (WT). After 500 mM NaCl treatment for 7 days, the activities of superoxide dismutase (SOD) and peroxidase (POD) and water content of the transgenic plants were higher than the WT, while malondialdehyde (MDA) was decreased in the transgenic plants. A total of 1082 and 1072 proteins were profiled by proteomics under control and salt treatment, respectively, with 22 and 11 proteins uniquely identified under control and salt stress, respectively. Among the 11 proteins, 7 were increased and 4 were decreased after salt treatment. Most of the proteins whose expression increased in the McHB7 overexpression (OE) ice plants under high salinity were involved in transport regulation, catalytic activities, biosynthesis of secondary metabolites, and response to stimulus. The results demonstrate that the McHB7 transcription factor plays a positive role in improving plant salt tolerance.
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spelling pubmed-82326862021-06-26 Proteomics of Homeobox7 Enhanced Salt Tolerance in Mesembryanthemum crystallinum Zhang, Xuemei Tan, Bowen Zhu, Dan Dufresne, Daniel Jiang, Tingbo Chen, Sixue Int J Mol Sci Article Mesembryanthemum crystallinum (common ice plant) is a halophyte species that has adapted to extreme conditions. In this study, we cloned a McHB7 transcription factor gene from the ice plant. The expression of McHB7 was significantly induced by 500 mM NaCl and it reached the peak under salt treatment for 7 days. The McHB7 protein was targeted to the nucleus. McHB7-overexpressing in ice plant leaves through Agrobacterium-mediated transformation led to 25 times more McHB7 transcripts than the non-transformed wild type (WT). After 500 mM NaCl treatment for 7 days, the activities of superoxide dismutase (SOD) and peroxidase (POD) and water content of the transgenic plants were higher than the WT, while malondialdehyde (MDA) was decreased in the transgenic plants. A total of 1082 and 1072 proteins were profiled by proteomics under control and salt treatment, respectively, with 22 and 11 proteins uniquely identified under control and salt stress, respectively. Among the 11 proteins, 7 were increased and 4 were decreased after salt treatment. Most of the proteins whose expression increased in the McHB7 overexpression (OE) ice plants under high salinity were involved in transport regulation, catalytic activities, biosynthesis of secondary metabolites, and response to stimulus. The results demonstrate that the McHB7 transcription factor plays a positive role in improving plant salt tolerance. MDPI 2021-06-15 /pmc/articles/PMC8232686/ /pubmed/34203768 http://dx.doi.org/10.3390/ijms22126390 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Xuemei
Tan, Bowen
Zhu, Dan
Dufresne, Daniel
Jiang, Tingbo
Chen, Sixue
Proteomics of Homeobox7 Enhanced Salt Tolerance in Mesembryanthemum crystallinum
title Proteomics of Homeobox7 Enhanced Salt Tolerance in Mesembryanthemum crystallinum
title_full Proteomics of Homeobox7 Enhanced Salt Tolerance in Mesembryanthemum crystallinum
title_fullStr Proteomics of Homeobox7 Enhanced Salt Tolerance in Mesembryanthemum crystallinum
title_full_unstemmed Proteomics of Homeobox7 Enhanced Salt Tolerance in Mesembryanthemum crystallinum
title_short Proteomics of Homeobox7 Enhanced Salt Tolerance in Mesembryanthemum crystallinum
title_sort proteomics of homeobox7 enhanced salt tolerance in mesembryanthemum crystallinum
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8232686/
https://www.ncbi.nlm.nih.gov/pubmed/34203768
http://dx.doi.org/10.3390/ijms22126390
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