Cargando…
Characterization of Growth and Cell Cycle Events Affected by Light Intensity in the Green Alga Parachlorella kessleri: A New Model for Cell Cycle Research
Multiple fission is a cell cycle variation leading to the production of more than two daughter cells. Here, we used synchronized cultures of the chlorococcal green alga Parachlorella kessleri to study its growth and pattern of cell division under varying light intensities. The time courses of DNA re...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8232753/ https://www.ncbi.nlm.nih.gov/pubmed/34203860 http://dx.doi.org/10.3390/biom11060891 |
_version_ | 1783713705055223808 |
---|---|
author | Zachleder, Vilém Ivanov, Ivan N. Kselíková, Veronika Bialevich, Vitali Vítová, Milada Ota, Shuhei Takeshita, Tsuyoshi Kawano, Shigeyuki Bišová, Kateřina |
author_facet | Zachleder, Vilém Ivanov, Ivan N. Kselíková, Veronika Bialevich, Vitali Vítová, Milada Ota, Shuhei Takeshita, Tsuyoshi Kawano, Shigeyuki Bišová, Kateřina |
author_sort | Zachleder, Vilém |
collection | PubMed |
description | Multiple fission is a cell cycle variation leading to the production of more than two daughter cells. Here, we used synchronized cultures of the chlorococcal green alga Parachlorella kessleri to study its growth and pattern of cell division under varying light intensities. The time courses of DNA replication, nuclear and cellular division, cell size, total RNA, protein content, dry matter and accumulation of starch were observed at incident light intensities of 110, 250 and 500 µmol photons m(−2)s(−1). Furthermore, we studied the effect of deuterated water on Parachlorella kessleri growth and division, to mimic the effect of stress. We describe a novel multiple fission cell cycle pattern characterized by multiple rounds of DNA replication leading to cell polyploidization. Once completed, multiple nuclear divisions were performed with each of them, immediately followed by protoplast fission, terminated by the formation of daughter cells. The multiple fission cell cycle was represented by several consecutive doublings of growth parameters, each leading to the start of a reproductive sequence. The number of growth doublings increased with increasing light intensity and led to division into more daughter cells. This study establishes the baseline for cell cycle research at the molecular level as well as for potential biotechnological applications, particularly directed synthesis of (deuterated) starch and/or neutral lipids as carbon and energy reserves. |
format | Online Article Text |
id | pubmed-8232753 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-82327532021-06-26 Characterization of Growth and Cell Cycle Events Affected by Light Intensity in the Green Alga Parachlorella kessleri: A New Model for Cell Cycle Research Zachleder, Vilém Ivanov, Ivan N. Kselíková, Veronika Bialevich, Vitali Vítová, Milada Ota, Shuhei Takeshita, Tsuyoshi Kawano, Shigeyuki Bišová, Kateřina Biomolecules Article Multiple fission is a cell cycle variation leading to the production of more than two daughter cells. Here, we used synchronized cultures of the chlorococcal green alga Parachlorella kessleri to study its growth and pattern of cell division under varying light intensities. The time courses of DNA replication, nuclear and cellular division, cell size, total RNA, protein content, dry matter and accumulation of starch were observed at incident light intensities of 110, 250 and 500 µmol photons m(−2)s(−1). Furthermore, we studied the effect of deuterated water on Parachlorella kessleri growth and division, to mimic the effect of stress. We describe a novel multiple fission cell cycle pattern characterized by multiple rounds of DNA replication leading to cell polyploidization. Once completed, multiple nuclear divisions were performed with each of them, immediately followed by protoplast fission, terminated by the formation of daughter cells. The multiple fission cell cycle was represented by several consecutive doublings of growth parameters, each leading to the start of a reproductive sequence. The number of growth doublings increased with increasing light intensity and led to division into more daughter cells. This study establishes the baseline for cell cycle research at the molecular level as well as for potential biotechnological applications, particularly directed synthesis of (deuterated) starch and/or neutral lipids as carbon and energy reserves. MDPI 2021-06-15 /pmc/articles/PMC8232753/ /pubmed/34203860 http://dx.doi.org/10.3390/biom11060891 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zachleder, Vilém Ivanov, Ivan N. Kselíková, Veronika Bialevich, Vitali Vítová, Milada Ota, Shuhei Takeshita, Tsuyoshi Kawano, Shigeyuki Bišová, Kateřina Characterization of Growth and Cell Cycle Events Affected by Light Intensity in the Green Alga Parachlorella kessleri: A New Model for Cell Cycle Research |
title | Characterization of Growth and Cell Cycle Events Affected by Light Intensity in the Green Alga Parachlorella kessleri: A New Model for Cell Cycle Research |
title_full | Characterization of Growth and Cell Cycle Events Affected by Light Intensity in the Green Alga Parachlorella kessleri: A New Model for Cell Cycle Research |
title_fullStr | Characterization of Growth and Cell Cycle Events Affected by Light Intensity in the Green Alga Parachlorella kessleri: A New Model for Cell Cycle Research |
title_full_unstemmed | Characterization of Growth and Cell Cycle Events Affected by Light Intensity in the Green Alga Parachlorella kessleri: A New Model for Cell Cycle Research |
title_short | Characterization of Growth and Cell Cycle Events Affected by Light Intensity in the Green Alga Parachlorella kessleri: A New Model for Cell Cycle Research |
title_sort | characterization of growth and cell cycle events affected by light intensity in the green alga parachlorella kessleri: a new model for cell cycle research |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8232753/ https://www.ncbi.nlm.nih.gov/pubmed/34203860 http://dx.doi.org/10.3390/biom11060891 |
work_keys_str_mv | AT zachledervilem characterizationofgrowthandcellcycleeventsaffectedbylightintensityinthegreenalgaparachlorellakesslerianewmodelforcellcycleresearch AT ivanovivann characterizationofgrowthandcellcycleeventsaffectedbylightintensityinthegreenalgaparachlorellakesslerianewmodelforcellcycleresearch AT kselikovaveronika characterizationofgrowthandcellcycleeventsaffectedbylightintensityinthegreenalgaparachlorellakesslerianewmodelforcellcycleresearch AT bialevichvitali characterizationofgrowthandcellcycleeventsaffectedbylightintensityinthegreenalgaparachlorellakesslerianewmodelforcellcycleresearch AT vitovamilada characterizationofgrowthandcellcycleeventsaffectedbylightintensityinthegreenalgaparachlorellakesslerianewmodelforcellcycleresearch AT otashuhei characterizationofgrowthandcellcycleeventsaffectedbylightintensityinthegreenalgaparachlorellakesslerianewmodelforcellcycleresearch AT takeshitatsuyoshi characterizationofgrowthandcellcycleeventsaffectedbylightintensityinthegreenalgaparachlorellakesslerianewmodelforcellcycleresearch AT kawanoshigeyuki characterizationofgrowthandcellcycleeventsaffectedbylightintensityinthegreenalgaparachlorellakesslerianewmodelforcellcycleresearch AT bisovakaterina characterizationofgrowthandcellcycleeventsaffectedbylightintensityinthegreenalgaparachlorellakesslerianewmodelforcellcycleresearch |