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In vitro and in vivo functions of SARS-CoV-2 infection-enhancing and neutralizing antibodies
SARS-CoV-2-neutralizing antibodies (NAbs) protect against COVID-19. A concern regarding SARS-CoV-2 antibodies is whether they mediate disease enhancement. Here, we isolated NAbs against the receptor-binding domain (RBD) or the N-terminal domain (NTD) of SARS-CoV-2 spike from individuals with acute o...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier Inc.
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8232969/ https://www.ncbi.nlm.nih.gov/pubmed/34242577 http://dx.doi.org/10.1016/j.cell.2021.06.021 |
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| author | Li, Dapeng Edwards, Robert J. Manne, Kartik Martinez, David R. Schäfer, Alexandra Alam, S. Munir Wiehe, Kevin Lu, Xiaozhi Parks, Robert Sutherland, Laura L. Oguin, Thomas H. McDanal, Charlene Perez, Lautaro G. Mansouri, Katayoun Gobeil, Sophie M.C. Janowska, Katarzyna Stalls, Victoria Kopp, Megan Cai, Fangping Lee, Esther Foulger, Andrew Hernandez, Giovanna E. Sanzone, Aja Tilahun, Kedamawit Jiang, Chuancang Tse, Longping V. Bock, Kevin W. Minai, Mahnaz Nagata, Bianca M. Cronin, Kenneth Gee-Lai, Victoria Deyton, Margaret Barr, Maggie Von Holle, Tarra Macintyre, Andrew N. Stover, Erica Feldman, Jared Hauser, Blake M. Caradonna, Timothy M. Scobey, Trevor D. Rountree, Wes Wang, Yunfei Moody, M. Anthony Cain, Derek W. DeMarco, C. Todd Denny, Thomas N. Woods, Christopher W. Petzold, Elizabeth W. Schmidt, Aaron G. Teng, I-Ting Zhou, Tongqing Kwong, Peter D. Mascola, John R. Graham, Barney S. Moore, Ian N. Seder, Robert Andersen, Hanne Lewis, Mark G. Montefiori, David C. Sempowski, Gregory D. Baric, Ralph S. Acharya, Priyamvada Haynes, Barton F. Saunders, Kevin O. |
| author_facet | Li, Dapeng Edwards, Robert J. Manne, Kartik Martinez, David R. Schäfer, Alexandra Alam, S. Munir Wiehe, Kevin Lu, Xiaozhi Parks, Robert Sutherland, Laura L. Oguin, Thomas H. McDanal, Charlene Perez, Lautaro G. Mansouri, Katayoun Gobeil, Sophie M.C. Janowska, Katarzyna Stalls, Victoria Kopp, Megan Cai, Fangping Lee, Esther Foulger, Andrew Hernandez, Giovanna E. Sanzone, Aja Tilahun, Kedamawit Jiang, Chuancang Tse, Longping V. Bock, Kevin W. Minai, Mahnaz Nagata, Bianca M. Cronin, Kenneth Gee-Lai, Victoria Deyton, Margaret Barr, Maggie Von Holle, Tarra Macintyre, Andrew N. Stover, Erica Feldman, Jared Hauser, Blake M. Caradonna, Timothy M. Scobey, Trevor D. Rountree, Wes Wang, Yunfei Moody, M. Anthony Cain, Derek W. DeMarco, C. Todd Denny, Thomas N. Woods, Christopher W. Petzold, Elizabeth W. Schmidt, Aaron G. Teng, I-Ting Zhou, Tongqing Kwong, Peter D. Mascola, John R. Graham, Barney S. Moore, Ian N. Seder, Robert Andersen, Hanne Lewis, Mark G. Montefiori, David C. Sempowski, Gregory D. Baric, Ralph S. Acharya, Priyamvada Haynes, Barton F. Saunders, Kevin O. |
| author_sort | Li, Dapeng |
| collection | PubMed |
| description | SARS-CoV-2-neutralizing antibodies (NAbs) protect against COVID-19. A concern regarding SARS-CoV-2 antibodies is whether they mediate disease enhancement. Here, we isolated NAbs against the receptor-binding domain (RBD) or the N-terminal domain (NTD) of SARS-CoV-2 spike from individuals with acute or convalescent SARS-CoV-2 or a history of SARS-CoV infection. Cryo-electron microscopy of RBD and NTD antibodies demonstrated function-specific modes of binding. Select RBD NAbs also demonstrated Fc receptor-γ (FcγR)-mediated enhancement of virus infection in vitro, while five non-neutralizing NTD antibodies mediated FcγR-independent in vitro infection enhancement. However, both types of infection-enhancing antibodies protected from SARS-CoV-2 replication in monkeys and mice. Three of 46 monkeys infused with enhancing antibodies had higher lung inflammation scores compared to controls. One monkey had alveolar edema and elevated bronchoalveolar lavage inflammatory cytokines. Thus, while in vitro antibody-enhanced infection does not necessarily herald enhanced infection in vivo, increased lung inflammation can rarely occur in SARS-CoV-2 antibody-infused macaques. |
| format | Online Article Text |
| id | pubmed-8232969 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2021 |
| publisher | Elsevier Inc. |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-82329692021-06-28 In vitro and in vivo functions of SARS-CoV-2 infection-enhancing and neutralizing antibodies Li, Dapeng Edwards, Robert J. Manne, Kartik Martinez, David R. Schäfer, Alexandra Alam, S. Munir Wiehe, Kevin Lu, Xiaozhi Parks, Robert Sutherland, Laura L. Oguin, Thomas H. McDanal, Charlene Perez, Lautaro G. Mansouri, Katayoun Gobeil, Sophie M.C. Janowska, Katarzyna Stalls, Victoria Kopp, Megan Cai, Fangping Lee, Esther Foulger, Andrew Hernandez, Giovanna E. Sanzone, Aja Tilahun, Kedamawit Jiang, Chuancang Tse, Longping V. Bock, Kevin W. Minai, Mahnaz Nagata, Bianca M. Cronin, Kenneth Gee-Lai, Victoria Deyton, Margaret Barr, Maggie Von Holle, Tarra Macintyre, Andrew N. Stover, Erica Feldman, Jared Hauser, Blake M. Caradonna, Timothy M. Scobey, Trevor D. Rountree, Wes Wang, Yunfei Moody, M. Anthony Cain, Derek W. DeMarco, C. Todd Denny, Thomas N. Woods, Christopher W. Petzold, Elizabeth W. Schmidt, Aaron G. Teng, I-Ting Zhou, Tongqing Kwong, Peter D. Mascola, John R. Graham, Barney S. Moore, Ian N. Seder, Robert Andersen, Hanne Lewis, Mark G. Montefiori, David C. Sempowski, Gregory D. Baric, Ralph S. Acharya, Priyamvada Haynes, Barton F. Saunders, Kevin O. Cell Article SARS-CoV-2-neutralizing antibodies (NAbs) protect against COVID-19. A concern regarding SARS-CoV-2 antibodies is whether they mediate disease enhancement. Here, we isolated NAbs against the receptor-binding domain (RBD) or the N-terminal domain (NTD) of SARS-CoV-2 spike from individuals with acute or convalescent SARS-CoV-2 or a history of SARS-CoV infection. Cryo-electron microscopy of RBD and NTD antibodies demonstrated function-specific modes of binding. Select RBD NAbs also demonstrated Fc receptor-γ (FcγR)-mediated enhancement of virus infection in vitro, while five non-neutralizing NTD antibodies mediated FcγR-independent in vitro infection enhancement. However, both types of infection-enhancing antibodies protected from SARS-CoV-2 replication in monkeys and mice. Three of 46 monkeys infused with enhancing antibodies had higher lung inflammation scores compared to controls. One monkey had alveolar edema and elevated bronchoalveolar lavage inflammatory cytokines. Thus, while in vitro antibody-enhanced infection does not necessarily herald enhanced infection in vivo, increased lung inflammation can rarely occur in SARS-CoV-2 antibody-infused macaques. Elsevier Inc. 2021-08-05 2021-06-18 /pmc/articles/PMC8232969/ /pubmed/34242577 http://dx.doi.org/10.1016/j.cell.2021.06.021 Text en © 2021 Elsevier Inc. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
| spellingShingle | Article Li, Dapeng Edwards, Robert J. Manne, Kartik Martinez, David R. Schäfer, Alexandra Alam, S. Munir Wiehe, Kevin Lu, Xiaozhi Parks, Robert Sutherland, Laura L. Oguin, Thomas H. McDanal, Charlene Perez, Lautaro G. Mansouri, Katayoun Gobeil, Sophie M.C. Janowska, Katarzyna Stalls, Victoria Kopp, Megan Cai, Fangping Lee, Esther Foulger, Andrew Hernandez, Giovanna E. Sanzone, Aja Tilahun, Kedamawit Jiang, Chuancang Tse, Longping V. Bock, Kevin W. Minai, Mahnaz Nagata, Bianca M. Cronin, Kenneth Gee-Lai, Victoria Deyton, Margaret Barr, Maggie Von Holle, Tarra Macintyre, Andrew N. Stover, Erica Feldman, Jared Hauser, Blake M. Caradonna, Timothy M. Scobey, Trevor D. Rountree, Wes Wang, Yunfei Moody, M. Anthony Cain, Derek W. DeMarco, C. Todd Denny, Thomas N. Woods, Christopher W. Petzold, Elizabeth W. Schmidt, Aaron G. Teng, I-Ting Zhou, Tongqing Kwong, Peter D. Mascola, John R. Graham, Barney S. Moore, Ian N. Seder, Robert Andersen, Hanne Lewis, Mark G. Montefiori, David C. Sempowski, Gregory D. Baric, Ralph S. Acharya, Priyamvada Haynes, Barton F. Saunders, Kevin O. In vitro and in vivo functions of SARS-CoV-2 infection-enhancing and neutralizing antibodies |
| title | In vitro and in vivo functions of SARS-CoV-2 infection-enhancing and neutralizing antibodies |
| title_full | In vitro and in vivo functions of SARS-CoV-2 infection-enhancing and neutralizing antibodies |
| title_fullStr | In vitro and in vivo functions of SARS-CoV-2 infection-enhancing and neutralizing antibodies |
| title_full_unstemmed | In vitro and in vivo functions of SARS-CoV-2 infection-enhancing and neutralizing antibodies |
| title_short | In vitro and in vivo functions of SARS-CoV-2 infection-enhancing and neutralizing antibodies |
| title_sort | in vitro and in vivo functions of sars-cov-2 infection-enhancing and neutralizing antibodies |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8232969/ https://www.ncbi.nlm.nih.gov/pubmed/34242577 http://dx.doi.org/10.1016/j.cell.2021.06.021 |
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