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Optimized immunofluorescence staining protocol for imaging germinal centers in secondary lymphoid tissues of vaccinated mice
Location of immune cells that form the germinal center reaction within secondary lymphoid tissues can be characterized using confocal microscopy. Here, we present an optimized immunofluorescence staining protocol to image germinal center structures in fixed/frozen spleen sections from ChAdOx1 nCoV-1...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8233161/ https://www.ncbi.nlm.nih.gov/pubmed/34195671 http://dx.doi.org/10.1016/j.xpro.2021.100499 |
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author | Fra-Bido, Sigrid Walker, Simon A. Innocentin, Silvia Linterman, Michelle A. |
author_facet | Fra-Bido, Sigrid Walker, Simon A. Innocentin, Silvia Linterman, Michelle A. |
author_sort | Fra-Bido, Sigrid |
collection | PubMed |
description | Location of immune cells that form the germinal center reaction within secondary lymphoid tissues can be characterized using confocal microscopy. Here, we present an optimized immunofluorescence staining protocol to image germinal center structures in fixed/frozen spleen sections from ChAdOx1 nCoV-19 immunized mice. This protocol can be adapted to identify other cell types within secondary lymphoid tissues. For complete information on the generation and use of this protocol to examine immune responses to the COVID vaccine ChAdOx1 nCoV-19, please refer to Silva-Cayetano et al. (2020). |
format | Online Article Text |
id | pubmed-8233161 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-82331612021-06-29 Optimized immunofluorescence staining protocol for imaging germinal centers in secondary lymphoid tissues of vaccinated mice Fra-Bido, Sigrid Walker, Simon A. Innocentin, Silvia Linterman, Michelle A. STAR Protoc Protocol Location of immune cells that form the germinal center reaction within secondary lymphoid tissues can be characterized using confocal microscopy. Here, we present an optimized immunofluorescence staining protocol to image germinal center structures in fixed/frozen spleen sections from ChAdOx1 nCoV-19 immunized mice. This protocol can be adapted to identify other cell types within secondary lymphoid tissues. For complete information on the generation and use of this protocol to examine immune responses to the COVID vaccine ChAdOx1 nCoV-19, please refer to Silva-Cayetano et al. (2020). Elsevier 2021-06-18 /pmc/articles/PMC8233161/ /pubmed/34195671 http://dx.doi.org/10.1016/j.xpro.2021.100499 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Protocol Fra-Bido, Sigrid Walker, Simon A. Innocentin, Silvia Linterman, Michelle A. Optimized immunofluorescence staining protocol for imaging germinal centers in secondary lymphoid tissues of vaccinated mice |
title | Optimized immunofluorescence staining protocol for imaging germinal centers in secondary lymphoid tissues of vaccinated mice |
title_full | Optimized immunofluorescence staining protocol for imaging germinal centers in secondary lymphoid tissues of vaccinated mice |
title_fullStr | Optimized immunofluorescence staining protocol for imaging germinal centers in secondary lymphoid tissues of vaccinated mice |
title_full_unstemmed | Optimized immunofluorescence staining protocol for imaging germinal centers in secondary lymphoid tissues of vaccinated mice |
title_short | Optimized immunofluorescence staining protocol for imaging germinal centers in secondary lymphoid tissues of vaccinated mice |
title_sort | optimized immunofluorescence staining protocol for imaging germinal centers in secondary lymphoid tissues of vaccinated mice |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8233161/ https://www.ncbi.nlm.nih.gov/pubmed/34195671 http://dx.doi.org/10.1016/j.xpro.2021.100499 |
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