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Optimized immunofluorescence staining protocol for imaging germinal centers in secondary lymphoid tissues of vaccinated mice

Location of immune cells that form the germinal center reaction within secondary lymphoid tissues can be characterized using confocal microscopy. Here, we present an optimized immunofluorescence staining protocol to image germinal center structures in fixed/frozen spleen sections from ChAdOx1 nCoV-1...

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Detalles Bibliográficos
Autores principales:	Fra-Bido, Sigrid, Walker, Simon A., Innocentin, Silvia, Linterman, Michelle A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8233161/ https://www.ncbi.nlm.nih.gov/pubmed/34195671 http://dx.doi.org/10.1016/j.xpro.2021.100499

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