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Using RNA-binding proteins for immunoprecipitation of mRNAs from Xenopus laevis embryos

This protocol is developed for identifying mRNAs that form complexes with mRNA-binding proteins (mRBPs) in Xenopus laevis embryos at different developmental stages. Here, we describe the use of the Ybx1 mRBP for immunoprecipitation-based mRNA isolation. This protocol features the translation of the...

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Detalles Bibliográficos
Autores principales: Martynova, Natalia Y., Parshina, Elena A., Zaraisky, Andrey G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8233257/
https://www.ncbi.nlm.nih.gov/pubmed/34195668
http://dx.doi.org/10.1016/j.xpro.2021.100552