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Telomeric DNA sequences in beetle taxa vary with species richness

Telomeres are protective structures at the ends of eukaryotic chromosomes, and disruption of their nucleoprotein composition usually results in genome instability and cell death. Telomeric DNA sequences have generally been found to be exceptionally conserved in evolution, and the most common pattern...

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Autores principales: Prušáková, Daniela, Peska, Vratislav, Pekár, Stano, Bubeník, Michal, Čížek, Lukáš, Bezděk, Aleš, Čapková Frydrychová, Radmila
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8233369/
https://www.ncbi.nlm.nih.gov/pubmed/34172809
http://dx.doi.org/10.1038/s41598-021-92705-y
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author Prušáková, Daniela
Peska, Vratislav
Pekár, Stano
Bubeník, Michal
Čížek, Lukáš
Bezděk, Aleš
Čapková Frydrychová, Radmila
author_facet Prušáková, Daniela
Peska, Vratislav
Pekár, Stano
Bubeník, Michal
Čížek, Lukáš
Bezděk, Aleš
Čapková Frydrychová, Radmila
author_sort Prušáková, Daniela
collection PubMed
description Telomeres are protective structures at the ends of eukaryotic chromosomes, and disruption of their nucleoprotein composition usually results in genome instability and cell death. Telomeric DNA sequences have generally been found to be exceptionally conserved in evolution, and the most common pattern of telomeric sequences across eukaryotes is (T(x)A(y)G(z))(n) maintained by telomerase. However, telomerase-added DNA repeats in some insect taxa frequently vary, show unusual features, and can even be absent. It has been speculated about factors that might allow frequent changes in telomere composition in Insecta. Coleoptera (beetles) is the largest of all insect orders and based on previously available data, it seemed that the telomeric sequence of beetles varies to a great extent. We performed an extensive mapping of the (TTAGG)(n) sequence, the ancestral telomeric sequence in Insects, across the main branches of Coleoptera. Our study indicates that the (TTAGG)(n) sequence has been repeatedly or completely lost in more than half of the tested beetle superfamilies. Although the exact telomeric motif in most of the (TTAGG)(n)-negative beetles is unknown, we found that the (TTAGG)(n) sequence has been replaced by two alternative telomeric motifs, the (TCAGG)(n) and (TTAGGG)(n), in at least three superfamilies of Coleoptera. The diversity of the telomeric motifs was positively related to the species richness of taxa, regardless of the age of the taxa. The presence/absence of the (TTAGG)(n) sequence highly varied within the Curculionoidea, Chrysomeloidea, and Staphylinoidea, which are the three most diverse superfamilies within Metazoa. Our data supports the hypothesis that telomere dysfunctions can initiate rapid genomic changes that lead to reproductive isolation and speciation.
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spelling pubmed-82333692021-07-06 Telomeric DNA sequences in beetle taxa vary with species richness Prušáková, Daniela Peska, Vratislav Pekár, Stano Bubeník, Michal Čížek, Lukáš Bezděk, Aleš Čapková Frydrychová, Radmila Sci Rep Article Telomeres are protective structures at the ends of eukaryotic chromosomes, and disruption of their nucleoprotein composition usually results in genome instability and cell death. Telomeric DNA sequences have generally been found to be exceptionally conserved in evolution, and the most common pattern of telomeric sequences across eukaryotes is (T(x)A(y)G(z))(n) maintained by telomerase. However, telomerase-added DNA repeats in some insect taxa frequently vary, show unusual features, and can even be absent. It has been speculated about factors that might allow frequent changes in telomere composition in Insecta. Coleoptera (beetles) is the largest of all insect orders and based on previously available data, it seemed that the telomeric sequence of beetles varies to a great extent. We performed an extensive mapping of the (TTAGG)(n) sequence, the ancestral telomeric sequence in Insects, across the main branches of Coleoptera. Our study indicates that the (TTAGG)(n) sequence has been repeatedly or completely lost in more than half of the tested beetle superfamilies. Although the exact telomeric motif in most of the (TTAGG)(n)-negative beetles is unknown, we found that the (TTAGG)(n) sequence has been replaced by two alternative telomeric motifs, the (TCAGG)(n) and (TTAGGG)(n), in at least three superfamilies of Coleoptera. The diversity of the telomeric motifs was positively related to the species richness of taxa, regardless of the age of the taxa. The presence/absence of the (TTAGG)(n) sequence highly varied within the Curculionoidea, Chrysomeloidea, and Staphylinoidea, which are the three most diverse superfamilies within Metazoa. Our data supports the hypothesis that telomere dysfunctions can initiate rapid genomic changes that lead to reproductive isolation and speciation. Nature Publishing Group UK 2021-06-25 /pmc/articles/PMC8233369/ /pubmed/34172809 http://dx.doi.org/10.1038/s41598-021-92705-y Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Prušáková, Daniela
Peska, Vratislav
Pekár, Stano
Bubeník, Michal
Čížek, Lukáš
Bezděk, Aleš
Čapková Frydrychová, Radmila
Telomeric DNA sequences in beetle taxa vary with species richness
title Telomeric DNA sequences in beetle taxa vary with species richness
title_full Telomeric DNA sequences in beetle taxa vary with species richness
title_fullStr Telomeric DNA sequences in beetle taxa vary with species richness
title_full_unstemmed Telomeric DNA sequences in beetle taxa vary with species richness
title_short Telomeric DNA sequences in beetle taxa vary with species richness
title_sort telomeric dna sequences in beetle taxa vary with species richness
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8233369/
https://www.ncbi.nlm.nih.gov/pubmed/34172809
http://dx.doi.org/10.1038/s41598-021-92705-y
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